Prevalence of CagA and antimicrobial sensitivity of H. pylori isolates of patients with gastric cancer in Egypt

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorAl-Eraky D.M.
dc.contributor.authorHelmy O.M.
dc.contributor.authorRagab Y.M.
dc.contributor.authorAbdul-Khalek Z.
dc.contributor.authorEl-Seidi E.A.
dc.contributor.authorRamadan M.A.
dc.contributor.otherDepartment of Microbiology and Immunology
dc.contributor.otherOctober University for Modern Sciences and Arts
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Medical Microbiology and Immunology
dc.contributor.otherFaculty of Medicine
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:54Z
dc.date.available2020-01-09T20:40:54Z
dc.date.issued2018
dc.descriptionScopus
dc.description.abstractBackground: Helicobacter pylori (H. pylori) infection has been recognized as a significant threat for gastric cancer. However, studies that investigated the oncogenic factors and antimicrobial resistance of H. pylori in Egyptian isolates with gastric cancer are rare. The current study aimed to examine: (1) The pattern of antimicrobial resistance of H. pylori isolates of Egyptian gastric cancer patients, and (2) the prevalence of Cytotoxin-associated gene A (CagA). Methods: Samples were collected from patients with gastric cancer. Isolation of H. pylori was performed using Columbia blood agar supplemented with 10% horse blood, and selective supplement of H. pylori for 3 to 5 days at 37 �C under microaerophilic condition. Isolates were identified by biochemical traits of H. pylori: oxidase, urease and catalase tests. Antimicrobial susceptibility of H. pylori isolates was examined against five antimicrobial agents using disc diffusion method. After that, extraction of DNA and Polymerase Chain Reaction (PCR) were performed to amplify the target genes. Results: Twelve samples were collected from six males and six females Egyptian patients with cancer with an age range from 22 to 65 years. These cases are scarce and samples were collected over a period of almost eleven months. All isolates were confirmed as positive H. pylori through colony morphology and biochemical tests. The most effective antibiotic found was ciprofloxacin whereas all isolates showed resistance to metronidazole and erythromycin. The target CagA oncogene gene with expected product size was reported and seven (out of twelve) isolates (58%) were identified as CagA positive. Conclusion: The current study is unique in two main aspects. First, it reported the pattern of antimicrobial susceptibility and prevalence of CagA gene in H. pylori from Egyptian patients. Second, it exclusively recruited isolates from gastric cancer patients which were confirmed by clinical and laparoscopic examination. The moderately high prevalence of CagA gene in Egyptian cancer patients calls for more vigilance against that oncogene. � 2018 The Author(s).en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=5700191215&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1186/s13027-018-0198-1
dc.identifier.doiPubMed ID :
dc.identifier.issn17509378
dc.identifier.otherhttps://doi.org/10.1186/s13027-018-0198-1
dc.identifier.otherPubMed ID :
dc.identifier.urihttps://t.ly/epML3
dc.language.isoEnglishen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.ispartofseriesInfectious Agents and Cancer
dc.relation.ispartofseries13
dc.subjectAntimicrobial sensitivityen_US
dc.subjectCagAen_US
dc.subjectEgypten_US
dc.subjectGastric canceren_US
dc.subjectH. pylorien_US
dc.subjectamoxicillinen_US
dc.subjectciprofloxacinen_US
dc.subjectcytotoxin associated gene Aen_US
dc.subjecterythromycinen_US
dc.subjectmetronidazoleen_US
dc.subjectoncoproteinen_US
dc.subjecttetracyclineen_US
dc.subjectunclassified drugen_US
dc.subjectadulten_US
dc.subjectageden_US
dc.subjectalertnessen_US
dc.subjectantibiotic resistanceen_US
dc.subjectantibiotic sensitivityen_US
dc.subjectArticleen_US
dc.subjectbacterial strainen_US
dc.subjectbacterium colonyen_US
dc.subjectbacterium isolateen_US
dc.subjectbiochemical analysisen_US
dc.subjectCagA geneen_US
dc.subjectclinical articleen_US
dc.subjectcontrolled studyen_US
dc.subjectdisease eradicationen_US
dc.subjectdisk diffusionen_US
dc.subjectDNA extractionen_US
dc.subjectEgypten_US
dc.subjectfemaleen_US
dc.subjectgene amplificationen_US
dc.subjectgene identificationen_US
dc.subjectgene sequenceen_US
dc.subjectHelicobacter pylorien_US
dc.subjecthumanen_US
dc.subjectmaleen_US
dc.subjectoncogeneen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectprevalenceen_US
dc.subjectpriority journalen_US
dc.subjectstomach canceren_US
dc.titlePrevalence of CagA and antimicrobial sensitivity of H. pylori isolates of patients with gastric cancer in Egypten_US
dc.typeArticleen_US
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