Impact of certain Solanum species’s natural products as potent cytotoxic and anti-Inflammatory agents
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | Ezzat, Shahira M | |
| dc.contributor.author | A. Alsherbiny, Muhammad | |
| dc.contributor.author | S. Elsakhawy, Fatma | |
| dc.contributor.author | M. Kamel, Gehan | |
| dc.contributor.author | A. Abdel-Kawy, Mostafa | |
| dc.date.accessioned | 2019-10-24T08:43:39Z | |
| dc.date.available | 2019-10-24T08:43:39Z | |
| dc.date.issued | 2015 | |
| dc.description | MSA Google Scholar | |
| dc.description.abstract | The present study was conducted to evaluate both the cytotoxic and anti-inflammatory activities of ethanol extracts (T), and both n-butanol (B) and total glyco-alkaloid fractions (TGA) of Solanum seaforthianum Andr. (SS) and Solanum macrocarpon L. (SM) growing in Egypt. Cytotoxic activity was measured using sulforhodamine B (SRB) assay on prostate cancer cell line (PC-3), breast cancer cell line (MCF7), liver cancer cell line (HepG2) and human fibroblast cell line (HFB4) while anti-inflammatory activity was measured using formalin induced paw edema method. The highest cytotoxic potentiality was indicated for those of TGA fraction of S. seaforthianum Andr. on PC-3 cell line (IC50 = 0.28μg/ml ± 0.01) followed by its activity on MCF-7 cell line (IC50 = 2.84 μg/ml±0.20). On the other hand, the potency of TGA fractions of both species showed higher potency followed by n-butanol fractions where ethanol extracts showed lowest potency which is emphasizing the cytotoxic potentiality of the glyco-alkaloids. Based on the IC50s indicated for the different extracts and fractions on normal fibroblast cell line, considerable safety was indicated against prostate carcinoma rather than breast or hepatic carcinoma. TGA fraction of S. macrocarpon L. and of S. seaforthianum Andr. showed the highest anti-inflammatory activity with efficacy of 159 and 156%, respectively as compared to standard indomethacin. That’s why the TGA fraction of S. seaforthianum Andr. was subjected for isolation of individual alkaloids using different chromatographic techniques and identified using 1H and 13-CNMR spectroscopy beside Cochromatography with authentic samples as solamargine (A1), solasonine (A2) and solasodine (A3) which are firstly isolated from S. seaforthianum Andr. growing in Egypt. | en_US |
| dc.description.sponsorship | Journal of Medicinal Plants Research | en_US |
| dc.identifier.citation | Adedapo AA (2008). Anti-inflammatory and analgesic activities of the aqueous extract of Acacia karroo stem bark in experimental animals. Basic Clin. Pharmacol. Toxicol. 103(5):397-400. Baade PD, Youlden DR, Krnjacki LJ (2009). International epidemiology of prostate cancer: geographical distribution and secular trends. Mol. Nutr. Food Res. 53(2):171-184. Bushway RJ, Barden ES, Bushway AW, and Bushway AA (1980). The mass extraction of potato glycoalkaloids from blossoms. Am. Potato J. 57(4):175-180. Dey S, Soliman AS, Hablas A, Seifeldein IA, Ismail K, Ramadan M, Merajver SD (2010). Urban-rural differences in breast cancer incidence in Egypt (1999-2006). Breast 19(5):417-423. Dharmasiri MG (2003). Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo. J. Ethnopharmacol. 87(2- 3):199-206. Ferlay J, Bray F, Pisani P, Parkin DM (2004). GLOBOCAN 2002: Cancer Incidence. Mortality and Prevalence Worldwide. IARC Cancer Base No. 5, version 2.0., Lyon: IARC Press. Freedman LS, Edwards BK, Ries LAG, Young JL (2006). Cancer incidence in four member countries (Cyprus, Egypt, Israel, and Jordan) of the Middle East Cancer Consortium (MECC) compared with US SEER. Ikeda T, Tsumagari H, Honbu T, Nohara T (2003). Cytotoxic activity of steroidal glycosides from solanum plants. Biol. Pharm. Bull. 26(8):1198-201. Jemal A, Center MM, De Santis C, Ward EM (2010). Global patterns of cancer incidence and mortality rates and trends. Cancer Epidemiol. Biomark. Prev. 19(8):1893-1907. Kärber G (1931). Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. Exp. Pathol. Pharmakol. 162:480-482. Lin CC, Lin WC, Yang SR, Shieh DE (1995). Anti-inflammatory and hepatoprotective effects of Solanum alatum. Am. J. Chin. Med. 23(1):65-9. Milner SE, Brunton NP, Jones PW, O’Brien NM, Collins SG, Maguire AR (2011). Bioactivities of glycoalkaloids and their aglycones from Solanum species. J. Agric. Food Chem. 59(8):3454-3484. Nakamura T, Komori C, Lee Y, Hashimoto F, Yahara S, Nohara T, Ejima A (1996). Cytotoxic activities of Solanum steroidal glycosides. Biol. Pharm. Bull. 19(4):564-566. Ndebia EJ, Kamgang R, Nkeh-Chungaganye BN (2007). Analgesic and anti-inflammatory properties of aqueous extract from leaves of Solanum torvum (Solanaceae). Afr. J. Tradit. Complement. Altern. Med. 4(2):240-244. Shabana MM, Salama MM, Ezzat SM, Ismail LR (2013). In Vitro and In Vivo Anticancer Activity of the Fruit Peels of Solanum melongena L. against Hepatocellular Carcinoma. J. Carcinog. Mutagen. 4(3):149- 54. Skehan P, Storeng R, Scudiero D, Monks A, Mcmahon J, Vistica D (1990). New colorimetric cytotoxicity assay for anticancer drug screening. J. Natl. Cancer Inst. 82:1107-12. Vieira G Jr, Ferreira PM, Matos LG, Ferreira EC, Rodovalho W, Ferri PH, Ferreira HD, Costa EA (2003). Anti-inflammatory effect of Solanum lycocarpum fruits. Phytother. Res. 17(8):892-6. Young HW, Luo YL, Cheng HY, Hsieh WC, Liao JC, Peng WH (2005). Analgesic and anti-inflammatory activities of [6]-gingerol. J. Ethnopharmacol. 96(1):207-210. Zaidi JH, Qureshi IH, Arif M, Fatima I (1992). Trace Element Analysis of Food Spices by Inaa: II. Solanaceae, Liliaceae, Zingiberaceae and Apiaceae Families. Int. J. Environ. Anal. Chem. 48(1):33-40. | en_US |
| dc.identifier.doi | https://doi.org/10.5897/JMPR2015.5872 | |
| dc.identifier.issn | 1996-0875 | |
| dc.identifier.other | https://doi.org/10.5897/JMPR2015.5872 | |
| dc.identifier.uri | https://cutt.ly/gteawDq | |
| dc.language.iso | en | en_US |
| dc.publisher | Journal of Medicinal Plants Research | en_US |
| dc.relation.ispartofseries | Journal of Medicinal Plants Research;29 | |
| dc.subject | University of Solanum | en_US |
| dc.subject | Solanum Seaforthianum | en_US |
| dc.subject | Solanum Macrocarpon | en_US |
| dc.title | Impact of certain Solanum species’s natural products as potent cytotoxic and anti-Inflammatory agents | en_US |
| dc.type | Article | en_US |