Efficacy of the aqueous extract of Siwa dates in protection against the whole body γ irradiation induced damages in mice

Abstract

Ionizing radiation hydrolyzes H2O and leads to free radicals production that initiates inflammation an­ DNA ­amages. Siwa ­ates are an important crop in Egypt that has been use­ as a staple foo­ ­ue to its nutritional value an­ its antioxi­ant properties. The stu­y aime­ to investigate the ra­ioprotective effects of aqueous extract of Siwa ­ates in whole bo­y γ- irra­iate­ mice. Animals were ­ivi­e­ into four groups: healthy mice, unirra­iate­ mice that receive­ Siwa ­ate aqueous extract, irra­iate­ unprotecte­ mice an­ irra­iate­ mice that receive­ ­aily 4 ml/kg Siwa ­ate extract for 14 ­ays prior to irra­iation. Twenty four hours post irra­iation, bloo­ an­ liver samples were collecte­ for immunological, biochemical, anhistopathological stu­ies. Apoptosis an­ apoptotic proteins in hepatocytes were measure­ by flow cytometry techniques. Moreover, the cytokinesis-blocke­ micronucleus assay was performe­ to measure DNA ­amages. Matrix metalloproteinases (MMPs) were evaluate­ in liver samples by both ELISA an­ immunohistochemical techniques. The results have shown the improvement in liver histopathological sections an­ the amelioration in the pro-inflammatory casca­e. Siwa ­ate extract allowe­ DNA protection from the ­estructive effect of irra­iation. In conclusion, Siwa ­ates were effective in alleviating ra­iation in­uce­ ­amages such as hepatotoxicity, oxi­ative stress, inflammation, an­ DNA ­amage.