Phytochemical Identification Components of Coffee Arabica Extracts and Its Role in Alleviation Alterations of Tetracycline Induced Non-alcoholic Steatohepatitis (NASH) in rats.

Abstract

High doses of tetracycline (TET) caused serious health effects, including non-alcoholic steatohepatitis (NASH). We identified the bioactive content of Coffee Arabica extract and its possible role in the alleviation of NASH induced by TET in rats. Sixty male albino rats (150 ± 10 g) were allocated into six groups: Control, TET: it received TET orally (1 g/kg BW) for 8 days; group III (CAME): it received Coffea Arabica methanolic extract (CAME) orally (0.1 g/kg) for 4 weeks; group IV (treated): it received TET then treated with CAME for 4 weeks; group V (preventive): it received CAME for 4 weeks then received TET orally for 8 days, VI (Protected): it received TET and CAME orally for 8 days. The total phenolic in the aqueous, methanolic, and ethanolic extracts were 751.36, 1259.09, and 1146.82 mg GAE/g, respectively. The GC/MS analysis showed the highest content of chlorogenic acid. The CAME ameliorated the liver alterations in TET-intoxicated rats by reducing the indexes of liver function and lipid profile (p<0.001), enhanced antioxidant capacity. CAME preventive is the most effective in liver alteration as portal vein congestion, scattered apoptosis and inflammatory infiltration. The autophagy signals as mTOR and LC3 will be investigated for more explanation its mechanism of action.