Role of Trehalose during Recovery from Drought Stress in Micropropagated Banana (Musa spp.) Transplants.
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Date
2017
Journal Title
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Article
Publisher
RJPBCS RESEARCH JOURNAL PHARMACEUTICAL, BIOLOGICAL & CHEMICAL SCIENCES
Series Info
RESEARCH JOURNAL OF PHARMACEUTICAL BIOLOGICAL AND CHEMICAL SCIENCES;Volume: 8 Issue: 2 Pages: 1335-1345
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Abstract
Micropropagated banana (Musa spp.) plants are widely accepted by growers as valuable, pathogen-free stock materials. Water stress is believed to adversely affect growth and yield of banana plants, as banana plants requires water in sufficient quantity for their normal metabolic activities. Trehalose is one of the organic substances currently known to be involved in osmotic adjustment, a significant strategy for plant drought tolerance. The current study was aimed to assess the role of trehalose as a presoaking treatment at 0, 20, 60 or 100 mM in improving tolerance of banana plantlets under in vitro drought stress conditions and following transitional period required in preparation for planting in the field. The pretreatment of 20 mM trehalose enhanced growth of banana plantlets under in vitro drought stress conditions in terms of root system and fresh weight. Non of in vitro stressed un-pretreated plantlets succeeded to continue grow during recovery stage in the green house. Results showed markedly higher indicators of growth recovery in banana transplants previously supplied with 20 mM trehalose. This growth improvement was accompanied with enhancement of chemical composition in terms of increasing pigments and reducing total free amino acids contents to reach almost normal levels in addition to higher total phenols content and overall antioxidant activity even more than control plants. Sodium Dodicyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis showed over expressed protein band of 32 kDa molecular weight in recovered transplants pretreated with the highest trehalose concentration (100 mM). Recovered transplants pretreated with trehalose at the lowest concentration (20 mM) showed balanced up regulation of both trehalose-6-phosphate synthase (TPS) and trehalase genes over control, while recovered transplants pretreated with trehalose at the highest concentration (100 mM) showed also over expression of TPS gene but down regulation of trehalase gene under control.
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Keywords
University of Banana, Trehalose, Drought stress, Recovery, Antioxidant activity, SDS-PAGE, TPS, Trehalase
Citation
Jan 200975-80 Ad BitarFh Mohamed [1] Bitar AD, Mohamed FH. Agricultural Research Journal Suez Canal University 2009; 9 (1): 75-80. [2] Mapping drought patterns and impacts: a global perspective [3] Egyptian Ministry of Water Resources and Irrigation. Water Scarcity in Egypt: The Urgent Need for Regional Cooperation among the Nile Basin Countries Jan 200931-36 N EriyagamaV SmakhtinN Gamage Eriyagama N, Smakhtin V, Gamage N. Mapping drought patterns and impacts: a global perspective. IWMI Research Report 133, Colombo, Sri Lanka, 2009, 31p. [3] Egyptian Ministry of Water Resources and Irrigation. Water Scarcity in Egypt: The Urgent Need for Regional Cooperation among the Nile Basin Countries, 2014, 5p. [4] Fang Y, Xiong L. Cell. Mol. Life Sci. 2015; 72: 673-689. [5] Combating abiotic stress using trehalose: Cross-protection in tissue culture of Arabidopsis thaliana Jan 200742-185 R StolkerM Ramon Stolker R. Combating abiotic stress using trehalose: Cross-protection in tissue culture of Arabidopsis thaliana. Thesis Report, Wageningen UR Plant Breeding, 2010, 42p. [6] Ramon M, Rolland F. TRENDS in Plant Science 2007; 12 (5): 185-188. Jan 1962PHYSIOL PLANTARUM473-497 T MurashigeFa Skoog Murashige T, Skoog FA. Physiologia Plantarum 1962;15: 473-497. [8] Jan 20151040-1046 Em SaidRa MahmoudR Al-AksharG Safwat Said EM, Mahmoud RA, Al-Akshar R, Safwat G. Austin J. Biotechnol. Bioeng. 2015; 2(2): 1040-1046. [9] In vitro propagation of Hansen 2168 rootstock. The 3 rd conference of recent technologies in agriculture Jan 2005556-574 Wt Saeed Saeed WT. In vitro propagation of Hansen 2168 rootstock. The 3 rd conference of recent technologies in agriculture, 2005, 556-574. [10] Jan 1954457-471 G HolmScand Acta AgrT SwainWe Hillis Holm G. Acta Agr. Scand. 1954; 4: 457-471. [12] Wettstein D. Cell Res. 1957; 12: 427-506. [13] Kawamura S. Tech. Bull. Fac. Agr. Kagawa Univ. 1967; 18(2): 117-131. [14] Miller GL. Anal. Chem. 1959; 31: 426-428. [15] Yemm EW, Cocking EC. Analyst 1955; 80: 209-214. [16] Swain T, Hillis WE. J. Sci. Food Agric. 1959; 10: 63-68. [17] Blois MS. Nature 2002; 26: 1199-1200. [18] Laemmli UK. Nature 1970; 227: 680-685. [19] Jan 1983DNA329-335 G CathalaJ SavouretB MendezBl WestM KarinJa MartialJd Baxter Cathala G, Savouret J, Mendez B, West BL, Karin M, Martial JA, Baxter JD. DNA 1983; 2: 329-335. [20] Jan 1997PLANT PHYSIOL181-190 Ojm GoddijnTc VerwoerdE VoogdRwhh KrutwagenPthm De GraafJ PoelsK Van DunAs PonsteinB Damm Goddijn OJM, Verwoerd TC, Voogd E, Krutwagen RWHH, de Graaf PTHM, Poels J, van Dun K, Ponstein AS, Damm B, Pen J. Plant physiology 1997; 113: 181-190. [26] Statistical Methods, Iowa State University Press Article Jan 1989 G. W. and SnedecorW. G. (Eds.). Cochran