The hidden mechanism beyond ginger (Zingiber officinale Rosc.) potent in vivo and in vitro anti-inflammatory activity

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorEzzat, Shahira M
dc.contributor.authorEzzat M.I.
dc.contributor.authorOkba M.M.
dc.contributor.authorMenze E.T.
dc.contributor.authorAbdel-Naim A.B.
dc.contributor.otherPharmacognosy Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherKasr El-Ainy Street
dc.contributor.otherCairo
dc.contributor.other11562
dc.contributor.otherEgypt; Department of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Science and Arts (MSA)
dc.contributor.other6th October
dc.contributor.other12566
dc.contributor.otherEgypt; Department of Pharmacology and Toxicology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAin Shams University
dc.contributor.otherCairo
dc.contributor.other11566
dc.contributor.otherEgypt; Department of Pharmacology and Toxicology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherKing Abdulaziz University
dc.contributor.otherJeddah
dc.contributor.otherSaudi Arabia
dc.date.accessioned2020-01-09T20:40:57Z
dc.date.available2020-01-09T20:40:57Z
dc.date.issued2018
dc.descriptionScopus
dc.descriptionMSA Google Scholar
dc.description.abstractEthnopharmacological relevance Ginger (Zingiber officinale Roscoe) is a well known anti-inflammatory drug in the Egyptian, Indian and Chinese folk medicines, yet its mechanism of action is unclear. Aim of the study To explore its mechanism of action and to correlate it to its biophytochemicals. Materials and methods Various extracts viz. water, 50%, 70%, 80%, and 90% ethanol were prepared from ginger rhizomes. Fractionation of the aqueous extract (AE) was accomplished using Diaion HP-20. In vitro anti-inflammatory activity of the different extracts and isolated compounds was evaluated using protein denaturation inhibition, membrane stabilization, protease inhibition, and anti-lipoxygenase assays. In vivo anti-inflammatory activity of AE was estimated using carrageenan-induced rat paw edema in rats at doses 25, 50, 100 and 200 mg/kg b.wt. Results All the tested extracts showed significant (p< 0.1) in vitro anti-inflammatory activities. The strongest anti-lipoxygenase activity was observed for AE that was more significant than that of diclofenac (58% and 52%, respectively) at the same concentration (125 ?g/ml). Purification of AE led to the isolation of 6-poradol (G1), 6-shogaol (G2); methyl 6- gingerol (G3), 5-gingerol (G4), 6-gingerol (G5), 8-gingerol (G6), 10-gingerol (G7), and 1-dehydro-6-gingerol (G8). G1, G2 and G8 exhibited potent activity in all the studied assays, while G4 and G5 exhibited moderate activity. In vivo administration of AE ameliorated rat paw edema in a dose-dependent manner. AE (at 200 mg/kg) showed significant reduction in production of PGE2, TNF-?, IL-6, monocyte chemoattractant protein-1 (MCP-1), regulated upon activation, normal T-cell expressed and secreted (RANTES), myeloperoxidase (MPO) activity by 60%, 57%, 60%, 41%, 32% and 67%, respectively. AE at 100 and 200 mg/kg was equipotent to indomethacin in reduction of NOx level and in increasing the total antioxidant capacity (TAC). Histopathological examination revealed very few inflammatory cells infiltration and edema after administration of AE (200 mg/kg) prior to carrageenan. Conclusions Ginger anti-inflammatory activity is mediated by inhibiting macrophage and neutrophils activation as well as negatively affecting monocyte and leukocyte migration. This was evidenced by the dose-dependent decrease in pro-inflammatory cytokines and chemokines and replenishment the total antioxidant capacity. � 2017 Elsevier B.V.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=23015&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1016/j.jep.2017.12.019
dc.identifier.doiPubMed ID 29253614
dc.identifier.issn3788741
dc.identifier.otherhttps://doi.org/10.1016/j.jep.2017.12.019
dc.identifier.otherPubMed ID 29253614
dc.identifier.urihttps://t.ly/MXmyV
dc.language.isoEnglishen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofseriesJournal of Ethnopharmacology
dc.relation.ispartofseries214
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectMSA University
dc.subjectUniversity for Modern Sciences and Arts
dc.subjectOctober University for Modern Sciences and Arts
dc.subject1-Dehydro-6-Gingerolen_US
dc.subject6-Shogaolen_US
dc.subjectAnti-lipoxygenase activityen_US
dc.subjectInflammatory markersen_US
dc.subject(10) gingerolen_US
dc.subject(6) gingerolen_US
dc.subject1 dehydro 6 gingerolen_US
dc.subject5 gingerolen_US
dc.subject6 poradolen_US
dc.subject6 shogaolen_US
dc.subject8 gingerolen_US
dc.subjectantiinflammatory agenten_US
dc.subjectdiclofenacen_US
dc.subjectginger extracten_US
dc.subjectindometacinen_US
dc.subjectinterferon receptoren_US
dc.subjectinterleukin 1alphaen_US
dc.subjectinterleukin 1betaen_US
dc.subjectinterleukin 6en_US
dc.subjectlipoxygenaseen_US
dc.subjectmethyl 6 gingerolen_US
dc.subjectmonocyte chemotactic protein 1en_US
dc.subjectmyeloperoxidaseen_US
dc.subjectprostaglandin E2en_US
dc.subjectproteinaseen_US
dc.subjectRANTESen_US
dc.subjecttumor necrosis factoren_US
dc.subjectunclassified drugen_US
dc.subjectantiinflammatory agenten_US
dc.subjectantioxidanten_US
dc.subjectautacoiden_US
dc.subjectcarrageenanen_US
dc.subjectcytokineen_US
dc.subjectlipoxygenase inhibitoren_US
dc.subjectplant extracten_US
dc.subjectprostaglandin E2en_US
dc.subjectproteinase inhibitoren_US
dc.subjectadulten_US
dc.subjectanimal cellen_US
dc.subjectanimal experimenten_US
dc.subjectanimal modelen_US
dc.subjectanimal tissueen_US
dc.subjectantiinflammatory activityen_US
dc.subjectantioxidant assayen_US
dc.subjectArticleen_US
dc.subjectcarrageenan-induced paw edemaen_US
dc.subjectcontrolled studyen_US
dc.subjectdose responseen_US
dc.subjectdrug isolationen_US
dc.subjectdrug mechanismen_US
dc.subjectdrug potencyen_US
dc.subjectenzyme inhibitionen_US
dc.subjectenzyme inhibition assayen_US
dc.subjectgingeren_US
dc.subjecthistopathologyen_US
dc.subjectIC50en_US
dc.subjectin vitro studyen_US
dc.subjectin vivo studyen_US
dc.subjectinflammatory infiltrateen_US
dc.subjectleukocyte migrationen_US
dc.subjectmacrophage activationen_US
dc.subjectmaleen_US
dc.subjectmembrane stabilizationen_US
dc.subjectmonocyteen_US
dc.subjectneutrophilen_US
dc.subjectnonhumanen_US
dc.subjectprotein denaturationen_US
dc.subjectraten_US
dc.subjectanimalen_US
dc.subjectchemically induceden_US
dc.subjectchemistryen_US
dc.subjectcomparative studyen_US
dc.subjectdisease modelen_US
dc.subjectdrug effecten_US
dc.subjectedemaen_US
dc.subjecterythrocyte membraneen_US
dc.subjecthumanen_US
dc.subjectinflammationen_US
dc.subjectisolation and purificationen_US
dc.subjectmacrophageen_US
dc.subjectmedicinal planten_US
dc.subjectmetabolismen_US
dc.subjectneutrophil chemotaxisen_US
dc.subjectphytotherapyen_US
dc.subjectplant rooten_US
dc.subjectSprague Dawley raten_US
dc.subjectAnimalsen_US
dc.subjectAnti-Inflammatory Agentsen_US
dc.subjectAntioxidantsen_US
dc.subjectCarrageenanen_US
dc.subjectChemotaxis, Leukocyteen_US
dc.subjectCytokinesen_US
dc.subjectDinoprostoneen_US
dc.subjectDisease Models, Animalen_US
dc.subjectDose-Response Relationship, Drugen_US
dc.subjectEdemaen_US
dc.subjectErythrocyte Membraneen_US
dc.subjectGingeren_US
dc.subjectHumansen_US
dc.subjectInflammationen_US
dc.subjectInflammation Mediatorsen_US
dc.subjectLipoxygenase Inhibitorsen_US
dc.subjectMacrophage Activationen_US
dc.subjectMacrophagesen_US
dc.subjectMaleen_US
dc.subjectMonocytesen_US
dc.subjectNeutrophilsen_US
dc.subjectPhytotherapyen_US
dc.subjectPlant Extractsen_US
dc.subjectPlant Rootsen_US
dc.subjectPlants, Medicinalen_US
dc.subjectProtease Inhibitorsen_US
dc.subjectRats, Sprague-Dawleyen_US
dc.titleThe hidden mechanism beyond ginger (Zingiber officinale Rosc.) potent in vivo and in vitro anti-inflammatory activityen_US
dc.typeArticleen_US
dcterms.isReferencedByAgarwal, M., Walia, S., Dhingra, S., Khambay, B.P.S., Insect growth inhibition, antifeedant and antifungal activity of compounds isolated/derived from Zingiber officinale Roscoe (ginger) rhizomes (2001) Pest Manag. Sci., 57, pp. 289-300; Alebouyeh, M., Pourpak, Z., Ahmadiani, A., Increase in serum level of interleukin-1 alpha mediates morphine anti-inflammatory effect in carrageenan-induced paw oedema in mice (2002) Cytokine, 19, pp. 102-105; Appay, V., Rowland-Jones, S.L., RANTES: a versatile and controversial chemokine (2001) Trends Immunol., 22, pp. 83-87; Banchroft, J.D., Stevens, A., Turner, D.R., Theory and Practice of Histological Techniques (1996), Churchil Livingstone New York, London, San Francisco, Tokyo; Bradely, P.P., Priebat, D.A., Christensen, R.D., Rothstein, G., Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker (1982) J. Investig. Dermatol., 78, pp. 206-209; Brown, J.H., Mackey, H.K., Inhibition of heat-induced denaturation of serum proteins by mixtures of non-steroidal anti-inflammatory agents and amino acids (1968) Proc. Soc. Exp. Biol. Med., 128, pp. 225-228; Charles, R., Garg, S.N., Kumar, S., New gingerdione from the rhizomes of Zingiber officinale (2000) Fitoterapia, 71, pp. 716-718; Connell, D., Sutherland, M., A re-examination of gingerol, shogaol and zingerone, the pungent principles of ginger (Zingiber officinale Roscoe) (1969) Aust. J. Chem., 22, pp. 1033-1043; Connell, D.W., McLachlan, R., Natural pungent compounds: examination of gingerols, shogaols, paradols and related compounds by thin-layer and gas chromatography (1972) J. Chromatogr., 67, pp. 29-35; Denniff, P., Macleod, I., Whiting, D.A., Studies in the biosynthesis of [6]-gingerol, pungent principle of ginger (Zingiber officinale) (1980) J. Chem. Soc. Perkin Trans., 1, pp. 2637-2641; Dugasani, S., Pichika, M.R., Nadarajah, V.D., Balijepalli, M.K., Tandra, S., Korlakunta, J.N., Comparative antioxidant and anti-inflammatory effects of [6]-gingerol, [8]-gingerol,[10]-gingerol and [6]-shogaol (2010) J. Ethnopharmacol., 127, pp. 515-520; El-Abhar, H.S., Hammad, L.N., Gawad, H.S., Modulating effect of ginger extract on rats with ulcerative colitis (2008) J. Ethnopharmacol., 118, pp. 367-372; Elsbach, P., Weiss, J., Phagocytic ceils: oxygen-independent antimicrobial systems (1988) Inflammation: Basic Principles and Clinical Correlates, pp. 445-470. , J.I. Gallin I.M. Goldstein R. Snyderman Raven Press New York; Gallin, J.I., Snyderman, R., (1999) Inflamation: Basic Principles and Clinical Correlates, pp. 1-3. , third ed Lippincott Williams & Wilkins Philadelphia; Ghayur, M.N., Gilani, A.H., Pharmacological basis for the medicinal use of ginger in gastrointestinal disorders (2005) Dig. Dis. Sci., 50, pp. 1889-1897; Goulet, J.L., Snouweart, J.N., Latour, A.M., Coffman, T.M., Altered inflammatory responses in leukotriene-deficient mice (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 12852-12856; He, X.G., Bernart, M.W., Lian, L.S., Lin, L.Z., High performance liquid chromatography-electrospray mass spectrometric analysis of pungent constituents of ginger (1998) J. Chromatogr., 796, pp. 327-334; Kiuchi, F., Iwakami, S., Shibuya, M., Hanaoka, F., Sankawa, U., Inhibition of prostaglandin and leukotriene biosynthesis by gingerols and diarylheptanoids (1992) Chem. Pharm. Bull., 40, pp. 387-391; Kiuchi, F., Shibuya, M., Sankawa, U., Inhibitors of prostaglandin biosynthesis from ginger (1982) Chem. Pharm. Bull., 30, pp. 754-757; Ko, J.K.S., Sham, N.F., Guo, X., Cho, C.H., Beneficial intervention of experimental colitis by passive cigarette smoking through the modulation of cytokines in rats (2001) J. Investig. Med., 49, pp. 21-29; Koracevic, D., Koracevic, G., Djordjevic, V., Andrejevic, S., Cosic, V., Method for the measurement of antioxidant activity in human fluids (2001) J. Clin. Pathol., 54, pp. 356-361; K�hn, H., Chaitidis, P., Roffeis, J., Walther, M., Arachidonic Acid metabolites in the cardiovascular system: the role of lipoxygenase isoforms in atherogenesis with particular emphasis on vascular remodeling (2007) J. Cardiovasc. Pharmacol., 50, pp. 609-620; Langner, E., Greifenberg, S., Gruenwald, J., Ginger: history and use (1998) Adv. Ther., 15, pp. 25-44; Lowry, O.H., Rosebrough, N.J., Farr, A.L., RJ, R., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275; Mabberley, D.J., (1997) The Plant-book: A Portable Dictionary of the Vascular Plants, p. 767. , 2nd ed. Cambridge University Press Cambridge, U.K; Miranda, K.M., Espey, M.G., Wink, D.A., A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite (2001) Nitric Oxide, 5, pp. 62-71; Mizushima, Y., Kobayashi, M., Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins (1968) J. Pharm. Pharmacol., 20, pp. 169-173; Mnich, S.J., Veenhuizen, A.W., Monahan, J.B., Sheehan, K.C., Lynch, K.R., Isakson, P.C., Portanova, J.P., Characterization of a monoclonal antibody that neutralizes the activity of prostaglandin E2 (1995) J. Immunol., 155, pp. 4437-4444; Mustafa, T., Sirivastava, K.C., Ginger (Zingiber officinale) in migraine headache (1990) J. Ethanopharmacol., 29, pp. 267-273; Nurtjahja-Tjendraputra, E., Ammit, A.J., Roufogalis, B.D., Tran, V.H., Duke, C.C., Effective anti-platelet and COX-1 enzyme inhibitors from pungent constituents of ginger (2003) Thromb. Res., 111, pp. 259-265; Opie, E.L., On the relation of necrosis and inflammation to denaturation of proteins (1962) J. Exp. Med., 115, pp. 597-608; Oyedepo, O.O., Femurewas, A.J., Anti-protease and membrane stabilizing activities of extracts of Fagra santhoxiloides, Olax subscorpioides and Tetrapluera tetraptera (1995) Int. J. Pharmacog., 33, pp. 65-69; Plengsuriyakarn, T., Viyanant, V., Eursitthichai, V., Picha, P., Kupradinun, P., Itharat, A., Na-Bangchang, K., Anticancer activities against cholangiocarcinoma, toxicity and pharmacological activities of Thai medicinal plants in animal models (2012) BMC Complement. Altern. Med., 27. , (12:23); Ravindran, P.N., Sasikumar, B., Johnson, K.G., Ratnambal, M.J., Babu, K.N., Zachariah, J.T., Nair, R.R., Genetic resources of ginger (Zingiber officinale Rosc.) and its conservation in India (1994) Plant Genet. Resour. Newsl., pp. 1-4; Rollins, B.J., Monocyte chemoattractant protein 1: a potential regulator of monocyte recruitment in inflammatory disease (1996) Mol. Med. Today, 2, pp. 198-204; Sadique, J., Al-Rqobahs, W.A., Bughaith, EI-Gindi, A.R., The bioactivity of certain medicinal plants on the stabilisation of RBS membrane system (1989) Fitoterapia, 60, pp. 525-532; Sakat, S., Juvekar, A.R., Gambhire, M.N., In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn (2010) Int. J. Pharm. Pharm. Sci., 2, pp. 146-156; Scanlon, V.C., (2010), Sanders, T. Essentials of Anatomy and physiology. Davis F.A., Filadelphia 6th edition, 287; Shinde, U.A., Kulkarni, K.R., Phadke, A.S., Nair, A.M., Mungantiwar, A.A., Dikshit, V.J., Saraf, M.N., Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara (Roxb.) Loud. Wood Oil. (1999) Indian J. Exp. Biol., 37, pp. 258-261; Shinde, U.A., Phadke, A.S., Nari, A.M., Mungantiwar, A.A., Dikshit, V.J., Saraf, M.N., Membrane stabilization activity- a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil (1999) Fitoterapia, 70, pp. 251-257; Shirpoor, A., Zerehpoosh, M., Ansari, M.H.K., Kheradmand, F., Rasmi, Y., Ginger extract mitigates ethanol-induced changes of alpha and beta � myosin heavy chain isoforms gene expression and oxidative stress in the heart of male wistar rats (2017) DNA Repair, pp. 45-49; Shoji, N., Iwasa, A., Takemoto, T., Ishida, Y., Cardiotonic principles of ginger (Zingiber officinale Roscoe) (1982) J. Pharm. Sci., 71, pp. 1174-1175; Surh, Y.J., Park, K.K., Chun, K.S., Lee, L., Lee, E., Lee, S.S., Anti-tumor-promoting activities of selected pungent phenolic substances present in ginger (1999) J. Environ. Pathol., Toxicol. Oncol., 18, pp. 131-139; Srivas, K.C., Effects of aqueous extracts of onion, garlic and ginger on platelet aggregation and metabolism of arachidonic acid in the blood vascular system: in vitro study (1984) Prostaglandin Leukotriene Med., 13, pp. 227-235; Srivastava, K.C., Mustafa, T., Ginger (Zingiber officinale) in rheumatism and musculoskeletal disorders (1992) Med. Hypotheses, 39, pp. 342-348; Tachie, A.N., Dwuma-Badu, D., Ayim, J.S.K., Dabra, T.T., Hydroxyphenylalkanones from Amomun melegueta (1975) Phytochemistry, 14, pp. 853-854; Tjendraputra, E., Tran, V.H., Liu-Brennan, D., Roufogalis, B.D., Duke, C.C., Effect of ginger constituents and synthetic analogues on cyclooxygenase-2 enzyme in intact cells (2001) Bioorg. Chem., 29, pp. 156-163; Tripathi, S., Bruch, D., Kittur, D.S., Ginger extract inhibits LPS induced macrophage activation and function (2008) BMC Complement. Altern. Med., 8, p. 1; Tyler, V.E., (1993) The Honest Herbal, pp. 147-148. , 3rd ed Pharmaceutical Product Press New York, London, Norwood; Varma, K.R., Jain, T.C., Bhattacharyya, S.C., Structure and stereochemistry of zingiberol and Juniper camphor (1962) Tetrahedron, 18, p. 979; Virella, G., Immunoserology (1998) Medical Immunology, pp. 259-281. , G. Virella 4th ed Marcel Dekker Inc New York; Wallace, J.L., Keenan, C.M., Granger, D.N., Gastric ulceration induced by nonsteroidal antiinflammatory drugs is a neutrophil-dependent process (1990) Am. J. Physiol. 259(Gastrointestinal Liver Physiol. 22), pp. G462-G467; Winter, C.A., Risley, E.A., Nuss, G.W., Carrageenin-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs (1962) Proc. Soc. Exp. Biol. Med., 111, pp. 544-547; Wohlmuth, H., Leach, D.N., Smith, M.K., Myers, S.P., Gingerol content of diploid and tetraploid clones of ginger (Zingiber officinale Roscoe) (2005) J. Agric. Food Chem., 53, pp. 5772-5778; Zakaria, Z.A., Mohamad, A.S., Chear, C.T., Wong, Y.Y., Israf, D.A., Sulaiman, M.R., Antiinflammatory and antinociceptive activities of Zingiber zerumbet methanol extract in experimental model systems (2010) Med. Princ. Pract., 19, pp. 287-294
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