Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | Al Bratty, Mohammed | |
| dc.contributor.author | Alhazmi, Hassan A | |
| dc.contributor.author | Javed, Sadique A | |
| dc.contributor.author | Rehman, Zia Ur | |
| dc.contributor.author | Najmi, Asim | |
| dc.contributor.author | El-Sharkawy, Karam A | |
| dc.date.accessioned | 2021-11-24T06:52:02Z | |
| dc.date.available | 2021-11-24T06:52:02Z | |
| dc.date.issued | 19/11/2021 | |
| dc.description.abstract | e interaction behavior of Fe 3+ with transferrin and apotransferrin (iron-free form) was investigated in this study using affinity capillary electrophoresis. Change in the mass and charge of protein upon binding to the metal ion in the capillary tube led to variation in its migration time and was used to measure the noncovalent binding interactions by fast screening method. Acetanilide was used as the electroosmotic | en_US |
| dc.identifier.doi | https://doi.org/10.1155/2021/6987454 | |
| dc.identifier.other | https://doi.org/10.1155/2021/6987454 | |
| dc.identifier.uri | http://repository.msa.edu.eg/xmlui/handle/123456789/4778 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Hindawi | en_US |
| dc.relation.ispartofseries | Journal of Spectroscopy;Volume 2021 (2021), Article ID 6987454, 10 pages | |
| dc.subject | Metalloproteins | en_US |
| dc.subject | Apotransferrin | en_US |
| dc.subject | Transferrin | en_US |
| dc.subject | Capillary Electrophoresis | en_US |
| dc.title | Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis | en_US |
| dc.type | Article | en_US |