Sustainable recycling of poultry eggshell waste for the synthesis of calcium oxide nanoparticles and evaluating its antibacterial potency against food-borne pathogens
Date
2024-01
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Assiut University, Faculty of Veterinary Medicine, Department of Animal Medicine
Series Info
Journal of Advanced Veterinary Research;Volume 14, Issue 1, Pages 130 - 134January 2024
Doi
Scientific Journal Rankings
Abstract
Nanoparticles are considered new antibacterial agents with a potential broad range of applications. Recently,
the synthesis of bio-nanoparticles (NPs) from natural sources such as coral, Ostrea shell, and eggshell, has attracted considerable attention. Eggshells are organic waste, rich in calcium carbonate (CaCO3
), and it is an easy
method to reduce it into powder of nano size. Utilization of waste materials as a precursor for NPs synthesis
makes the entire process cheaper, greener, and more sustainable. Waste chicken eggshells were collected from
the Specific Pathogen Free farm in Egypt. Eggshells were rinsed, dried, and finely ground to powder. The sol-gel
chemical method was used for the synthesis of CaO-NPs from eggshell powder. The characteristics of eggshell
NPs were visualized using a scanning electron microscope, transmission electron microscope, Fourier transform
infrared spectroscopy, and ultraviolet–visible spectroscopy. Additionally, the minimum inhibitory concentration
was applied to test the antibacterial efficacy of CaO-NPs at 1.00, 0.50, 0.25, 0.12, and 0.06% concentrations on
Methicillin-resistant Staphylococcus aureus (MRSA), Bacillus cereus, Escherichia coli, and Salmonella enteritidis.
The results of the characterization confirmed the conversion of CaCO3
to CaO-NPs with an average diameter
of 27.7 nm. Zones of inhibition started to appear from 0.25% concentration for B. cereus, 0.50% for MRSA and
E. coli, and 1.0% for S. enteritidis. The concentration of CaO-NPs solution strongly correlated with the resulting
zone of inhibition (r= 0.86 to 0.90). CaO-NPs showed a potent efficacy against gram-positive bacteria. Hence,
eggshell wastes from poultry production could be a feasible organic source for the biosynthesis of CaO-NPs
with promising efficient antibacterial properties.
Description
Keywords
Calcium oxide; E. coli; Eggshell waste; FTIR; MRSA; Nano-antibacterial; Sol-gel