Phenolic profile of centaurea aegyptiaca L. Growing in Egypt and its cytotoxic and antiviral activities

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorBakr R.O.
dc.contributor.authorMohamed S.A.E.H.
dc.contributor.authorAyoub N.
dc.contributor.otherDepartment of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA)
dc.contributor.otherEgypt; Department of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAl-Azhar University (Girls)
dc.contributor.otherEgypt; Department of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherBritish University in Egypt (BUE)
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:42Z
dc.date.available2020-01-09T20:41:42Z
dc.date.issued2016
dc.descriptionScopus
dc.description.abstractBackground: Centaurea aegyptiaca L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the aerial parts of C. aegyptiaca methanolic extract utilizing liquid chromatography (LC) combined with electrospray ionization (ESI) tandem mass spectrometry (MS/MS). Material and Methods: Phenolic profile was researched utilizing LC-HRESI-MS-MS. Assessment of cytotoxic activity against four human cancer cell lines (Hep-G2; hepatocellular carcinoma cells, MCF-7; breast adenocarcinoma cells, and HCT-116; colon carcinoma and HELA; cervical carcinoma cells) was performed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Antiviral activity was surveyed utilizing cytopathic effect inhibition assay. Results: A total of sixty-one compounds were tentatively distinguished (twenty-one phenolic acids and their derivatives, thirty-one flavonols and nine flavones) in the negative and positive modes. Centaurea aegyptiaca demonstrated outstanding results against Hep-G2, MCF-7, HCT-116 and HELA cell lines with IC50of 12.1, 30.9, 11.7 and 19.5 ?g/mL respectively compared and doxorubicin as a reference drug. Weak antiviral activity was seen against hepatitis A virus (HAV) and no impact against herpes simplex virus type 1 (HSV 1). Conclusion: This study provides a better understanding of the chemistry of C. aegyptiaca that announces itself as a promising cytotoxic agent. 2016, African Ethnomedicines Network. All rights reserved.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=4700152608&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.21010/ajtcam.v13i6.19
dc.identifier.doiPubMed ID 28480370
dc.identifier.issn1896016
dc.identifier.otherhttps://doi.org/10.21010/ajtcam.v13i6.19
dc.identifier.otherPubMed ID 28480370
dc.identifier.urihttps://t.ly/7OJ7X
dc.language.isoEnglishen_US
dc.publisherAfrican Ethnomedicines Networken_US
dc.relation.ispartofseriesAfrican Journal of Traditional, Complementary and Alternative Medicines
dc.relation.ispartofseries13
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectUniversity of Modern Sciences and Arts
dc.subjectMSA University
dc.subjectCentaurea aegyptiacaen_US
dc.subjectCytotoxicityen_US
dc.subjectFlavonoiden_US
dc.subjectMTT assayen_US
dc.subject3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromideen_US
dc.subjectantineoplastic agenten_US
dc.subjectantivirus agenten_US
dc.subjectCentaurea aegyptiaca extracten_US
dc.subjectCentaurea aegyptiaca plant extracten_US
dc.subjectdoxorubicinen_US
dc.subjectflavone derivativeen_US
dc.subjectflavonolen_US
dc.subjectmethanolen_US
dc.subjectphenolen_US
dc.subjectplant extracten_US
dc.subjectunclassified drugen_US
dc.subjectantineoplastic agenten_US
dc.subjectantivirus agenten_US
dc.subjectphenol derivativeen_US
dc.subjectplant extracten_US
dc.subjectantineoplastic activityen_US
dc.subjectantiviral activityen_US
dc.subjectArticleen_US
dc.subjectcancer cell lineen_US
dc.subjectCentaureaen_US
dc.subjectCentaurea aegyptiacaen_US
dc.subjectchemical compositionen_US
dc.subjectcollisionally activated dissociationen_US
dc.subjectcontrolled studyen_US
dc.subjectdrug mechanismen_US
dc.subjectEgypten_US
dc.subjectelectrospray mass spectrometryen_US
dc.subjectHCT 116 cell lineen_US
dc.subjectHeLa cell lineen_US
dc.subjectHep-G2 cell lineen_US
dc.subjectHepatitis A virusen_US
dc.subjectHerpes simplex virus 1en_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectIC50en_US
dc.subjectliquid chromatographyen_US
dc.subjectMCF-7 cell lineen_US
dc.subjectMTT assayen_US
dc.subjectnonhumanen_US
dc.subjectphytochemistryen_US
dc.subjectaerial plant parten_US
dc.subjectchemistryen_US
dc.subjecttandem mass spectrometryen_US
dc.subjectAntineoplastic Agents, Phytogenicen_US
dc.subjectAntiviral Agentsen_US
dc.subjectCentaureaen_US
dc.subjectEgypten_US
dc.subjectHCT116 Cellsen_US
dc.subjectHeLa Cellsen_US
dc.subjectHep G2 Cellsen_US
dc.subjectHumansen_US
dc.subjectMCF-7 Cellsen_US
dc.subjectPhenolsen_US
dc.subjectPlant Components, Aerialen_US
dc.subjectPlant Extractsen_US
dc.subjectTandem Mass Spectrometryen_US
dc.titlePhenolic profile of centaurea aegyptiaca L. Growing in Egypt and its cytotoxic and antiviral activitiesen_US
dc.typeArticleen_US
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