Carriage frequency, phenotypic, and genotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from dental health-care personnel, patients, and environment

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorKhairalla A.S.
dc.contributor.authorWasfi R.
dc.contributor.authorAshour H.M.
dc.contributor.otherDepartment of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherBeni-Suef University Egypt
dc.contributor.otherBeni-Suef
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA)
dc.contributor.otherGiza
dc.contributor.otherEgypt; Department of Biological Sciences
dc.contributor.otherCollege of Arts and Sciences
dc.contributor.otherUniversity of South Florida St.Petersburg
dc.contributor.otherSt.Petersburg
dc.contributor.otherUnited States; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University Egypt
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:12Z
dc.date.available2020-01-09T20:41:12Z
dc.date.issued2017
dc.descriptionScopus
dc.description.abstractThere is limited data on methicillin-resistant Staphylococcus aureus (MRSA) carriage in dental clinics.1300 specimens from patients, health personnel, and environmental surfaces of a dental clinic in Egypt were tested for MRSA.Antibiotic susceptibility, biofilm formation, Staphylococcal protein A (spa) typing, SCCmec typing, and PCR-based assays were used to detect mecA, mecC, vanA, Panton-Valentine Leukocidin toxin (PVL), and toxic shock syndrome toxin-1 (tst) genes.Among 34 mecA-positive MRSA isolates, five (14.7%) were PVL-positive, seventeen (50%) were tst-positive, ten (29.4%) were vanA-positive, while none harboured mecC.MRSA hand carriage rates in patients, nurses, and dentists were 9.8%, 6.6%, and 5%.The respective nasal colonization rates were 11.1%, 6.7%, and 9.7%.1.3% of the environmental isolates were MRSA-positive.Strong and moderate biofilm-forming isolates represented 23.5% and 29.4% of MRSA isolates.24 MRSA isolates (70.6%) were multi-resistant and 18 (52.9%) harboured SCCmec IV.Among eight spa types, t223 (26.5%), t267 (23.5%), and t14339 (23.5%) were predominant.We noted an alarming genetic relatedness between 7 (20.6%) MRSA isolates and the epidemic EMRSA-15 clone, as well as a combined occurrence of tst and PVL in 3 (8.8%) isolates.Results suggest high MRSA pathogenicity in dental wards highlighting the need for more efficient surveillance/infection control strategies. � 2017 The Author(s).en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=21100200805&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1038/s41598-017-07713-8
dc.identifier.doiPubMed ID 28784993
dc.identifier.issn20452322
dc.identifier.otherhttps://doi.org/10.1038/s41598-017-07713-8
dc.identifier.otherPubMed ID 28784993
dc.identifier.urihttps://t.ly/Gggrd
dc.language.isoEnglishen_US
dc.publisherLife Science Journal
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofseriesScientific Reports
dc.relation.ispartofseries7
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectUniversity of Modern Sciences and Arts
dc.subjectMSA University
dc.subjectbacterial proteinen_US
dc.subjectbiofilmen_US
dc.subjectclassificationen_US
dc.subjectcross-sectional studyen_US
dc.subjectdental clinicen_US
dc.subjectEgypten_US
dc.subjectenvironmental microbiologyen_US
dc.subjectgeneticsen_US
dc.subjectgrowth, development and agingen_US
dc.subjecthanden_US
dc.subjecthealth care personnelen_US
dc.subjectheterozygoteen_US
dc.subjecthumanen_US
dc.subjectisolation and purificationen_US
dc.subjectmethicillin resistant Staphylococcus aureusen_US
dc.subjectmicrobial sensitivity testen_US
dc.subjectmicrobiologyen_US
dc.subjectmolecular evolutionen_US
dc.subjectmolecular typingen_US
dc.subjectnoseen_US
dc.subjectphylogenyen_US
dc.subjectproceduresen_US
dc.subjectStaphylococcus infectionen_US
dc.subjecttooth diseaseen_US
dc.subjecttransmissionen_US
dc.subjectBacterial Proteinsen_US
dc.subjectBiofilmsen_US
dc.subjectCarrier Stateen_US
dc.subjectCross-Sectional Studiesen_US
dc.subjectDental Clinicsen_US
dc.subjectEgypten_US
dc.subjectEnvironmental Microbiologyen_US
dc.subjectEvolution, Molecularen_US
dc.subjectHanden_US
dc.subjectHealth Personnelen_US
dc.subjectHumansen_US
dc.subjectMethicillin-Resistant Staphylococcus aureusen_US
dc.subjectMicrobial Sensitivity Testsen_US
dc.subjectMolecular Typingen_US
dc.subjectNoseen_US
dc.subjectPhylogenyen_US
dc.subjectStaphylococcal Infectionsen_US
dc.subjectTooth Diseasesen_US
dc.titleCarriage frequency, phenotypic, and genotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from dental health-care personnel, patients, and environmenten_US
dc.typeArticleen_US
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