Characterization of inflammatory breast cancer: a vibrational microspectroscopy and imaging approach at the cellular and tissue level
Date
2018
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Royal Society of Chemistry
Series Info
Analyst;Volume: 143 Issue: 24 Pages: 6103-6112
Scientific Journal Rankings
Abstract
Inflammatory breast cancer (IBC) has a poor prognosis because of the lack of specific biomarkers and its late diagnosis. An accurate and rapid diagnosis implemented early enough can significantly improve the disease outcome. Vibrational spectroscopy has proven to be useful for cell and tissue characterization based on the intrinsic molecular information. Here, we have applied infrared and Raman microspectroscopy and imaging to differentiate between non-IBC and IBC at both cell and tissue levels. Two human breast cancer cell lines (MDA-MB-231 and SUM-149), 20 breast cancer patients (10 non-IBC and 10 IBC), and 4 healthy volunteer biopsies were investigated. Fixed cells and tissues were analyzed by FTIR microspectroscopy and imaging, while live cells were studied by Raman microspectroscopy. Spectra were analyzed by hierarchical cluster analysis (HCA) and images by common k-means clustering algorithms. For both cell suspensions and single cells, FTIR spectroscopy showed sufficient high inter-group variability to delineate MDA-MB-231 and SUM-149 cell lines. Most significant differences were observed in the spectral regions of 1096–1108 and 1672–1692 cm−1. Analysis of live cells by Raman microspectroscopy gave also a good discrimination of these cell types. The most discriminant regions were 688–992, 1019–1114, 1217–1375 and 1516–1625 cm−1. Finally, k-means cluster analysis of FTIR images allowed delineating non-IBC from IBC tissues. This study demonstrates the potential of vibrational spectroscopy and imaging to discriminate between non-IBC and IBC at both cell and tissue levels.
Description
MSA Google Scholar
Keywords
University of Inflammatory Breast Cancer
Citation
H. T. Mohamed, M. El-Shinawi, M. A. Nouh, A. R. Bashtar, E. T. Elsayed, R. J. Schneider and M. M. Mohamed, Front. Oncol., 2014, 4, 246 CrossRef PubMed . D. A.-R. Mona Mostafa Mohamed, S. F. Sabet and M. El-Shinawi, J. Adv. Res., 2013, 5, 12 Search PubMed . J. M. Walshe and S. M. Swain, Breast Dis., 2005, 22, 35–44 Search PubMed . F. M. Robertson, M. Bondy, W. Yang, H. Yamauchi, S. Wiggins, S. Kamrudin, S. Krishnamurthy, H. Le-Petross, L. Bidaut, A. N. Player, S. H. Barsky, W. A. Woodward, T. Buchholz, A. Lucci, N. T. Ueno and M. Cristofanilli, CA-Cancer J. Clin., 2010, 60, 351–375 CrossRef PubMed . F. Peters, A. Kießlich and V. Pahnke, Eur. J. Obstet. Gynecol. Reprod. Biol., 2002, 105, 59–63 CrossRef . S. B. Edge and C. C. Compton, Ann. Surg. Oncol., 2010, 17, 1471–1474 CrossRef PubMed . M. I. Nounou, F. ElAmrawy, N. Ahmed, K. Abdelraouf, S. Goda and H. Syed-Sha-Qhattal, Breast Cancer, 2015, 9s2, 17–34 Search PubMed . A. N. Tosteson, D. G. Fryback, C. S. Hammond, L. G. Hanna, M. R. Grove, M. Brown, Q. Wang, K. Lindfors and E. D. Pisano, JAMA Intern. Med., 2014, 174, 954–961 CrossRef PubMed . N. Ragu, P. Roger and P. Taourel, J. Radiol., 2004, 85, 2069–2070 CrossRef CAS PubMed . C. G. Kleer, K. L. van Golen, T. Braun and S. D. Merajver, Mod. Pathol., 2001, 14, 458–464 CrossRef CAS PubMed . Y. Gong, Semin. Oncol., 2008, 35, 33–40 CrossRef CAS PubMed . P. J. Kowalski, M. A. Rubin and C. G. Kleer, Breast Cancer Res., 2003, 5, R217–R222 CrossRef CAS PubMed . H. Yamauchi, W. A. Woodward, V. Valero, R. H. Alvarez, A. Lucci, T. A. Buchholz, T. Iwamoto, S. Krishnamurthy, W. Yang, J. M. Reuben, G. N. Hortobagyi and N. T. Ueno, Oncologist, 2012, 17, 891–899 CrossRef PubMed . F. Lerebours, G. Cizeron-Clairac, A. Susini, S. Vacher, E. Mouret-Fourme, C. Belichard, E. Brain, J. L. Alberini, F. Spyratos, R. Lidereau and I. Bieche, Int. J. Cancer, 2013, 133, 1614–1623 CrossRef CAS PubMed . C. Kendall, M. Isabelle, F. Bazant-Hegemark, J. Hutchings, L. Orr, J. Babrah, R. Baker and N. Stone, Analyst, 2009, 134, 1029–1045 RSC . L. Quaroni and T. Zlateva, Analyst, 2011, 136, 3219–3232 RSC . A. D. Meade, O. Howe, V. Unterreiner, G. D. Sockalingum, H. J. Byrne and F. M. Lyng, Faraday Discuss., 2016, 187, 213–234 RSC . J. Nallala, M. D. Diebold, C. Gobinet, O. Bouche, G. D. Sockalingum, O. Piot and M. Manfait, Analyst, 2014, 139, 4005–4015 RSC . H. T. Mohamed, V. Untereiner, G. D. Sockalingum and S. Brezillon, Glycoconjugate J., 2017, 34, 309–323 CrossRef CAS PubMed . D. Sebiskveradze, V. Vrabie, P. Jeannesson, O. Piot and M. Manfait, Diagn. Pathol., 2013, 8, S33 CrossRef . P. Lasch, W. Haensch, D. Naumann and M. Diem, Biochim. Biophys. Acta, 2004, 1688, 176–186 CrossRef CAS PubMed . G. Bellisola and C. Sorio, Am. J. Cancer Res., 2012, 2, 1–21 CAS . C. Pezzei, J. D. Pallua, G. Schaefer, C. Seifarth, V. Huck-Pezzei, L. K. Bittner, H. Klocker, G. Bartsch, G. K. Bonn and C. W. Huck, Mol. BioSyst., 2010, 6, 2287–2295 RSC . D. Sebiskveradze, V. Vrabie, C. Gobinet, A. Durlach, P. Bernard, E. Ly, M. Manfait, P. Jeannesson and O. Piot, Lab. Invest., 2011, 91, 799–811 CrossRef CAS PubMed . U. Zelig, E. Barlev, O. Bar, I. Gross, F. Flomen, S. Mordechai, J. Kapelushnik, I. Nathan, H. Kashtan, N. Wasserberg and O. Madhala-Givon, BMC Cancer, 2015, 15, 408 CrossRef PubMed . S. Kumar, T. S. Shabi and E. Goormaghtigh, PLoS One, 2014, 9, e111137 CrossRef PubMed . M. H. Ali, F. Rakib, K. Al-Saad, R. Al-Saady, F. M. Lyng and E. Goormaghtigh, J. Mol. Struct., 2018, 1163, 472–479 CrossRef CAS . A. Mignolet, B. R. Wood and E. Goormaghtigh, Analyst, 2018, 143, 258–269 RSC . S. Kumar, C. Desmedt, D. Larsimont, C. Sotiriou and E. Goormaghtigh, Analyst, 2013, 138, 4058–4065 RSC . A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, R. Shenk, N. Wang, R. R. Dasari, M. Fitzmaurice and M. S. Feld, J. Biomed. Opt., 2009, 14, 054023 CrossRef PubMed . Y. Yang, F. Li, L. Gao, Z. Wang, M. J. Thrall, S. S. Shen, K. K. Wong and S. T. Wong, Biomed. Opt. Express, 2011, 2, 2160–2174 CrossRef PubMed . K. Kong, F. Zaabar, E. Rakha, I. Ellis, A. Koloydenko and I. Notingher, Phys. Med. Biol., 2014, 59, 6141–6152 CrossRef PubMed . T. Bhattacharjee, L. C. Fontana, L. Raniero and J. Ferreira-Strixino, J. Raman Spectrosc., 2018, 49, 786–791 CrossRef CAS . C. Zhang, P. T. Winnard Jr., S. Dasari, S. L. Kominsky, M. Doucet, S. Jayaraman, V. Raman and I. Barman, Chem. Sci., 2018, 9, 743–753 RSC . M. A. Nouh, M. M. Mohamed, M. El-Shinawi, M. A. Shaalan, D. Cavallo-Medved, H. M. Khaled and B. F. Sloane, J. Transl. Med., 2011, 9, 1 CrossRef CAS PubMed . F. Draux, C. Gobinet, J. Sule-Suso, A. Trussardi, M. Manfait, P. Jeannesson and G. D. Sockalingum, Anal. Bioanal. Chem., 2010, 397, 2727–2737 CrossRef CAS PubMed . E. Ly, O. Piot, R. Wolthuis, A. Durlach, P. Bernard and M. Manfait, Analyst, 2008, 133, 197–205 RSC . T. N. Nguyen, P. Jeannesson, A. Groh, O. Piot, D. Guenot and C. Gobinet, J. Biophotonics, 2016, 9, 521–532 CrossRef PubMed . Z. Movasaghi, S. Rehman and D. I. ur Rehman, Appl. Spectrosc. Rev., 2008, 43, 134–179 CrossRef CAS . Z. Movasaghi, S. Rehman and I. U. Rehman, Appl. Spectrosc. Rev., 2007, 42, 493–541 CrossRef CAS . M. J. Baker, J. l. Trevisan, P. Bassan, R. Bhargava, H. J. Butler, K. M. Dorling, P. R. Fielden, S. W. Fogarty, N. J. Fullwood and K. A. Heys, Nat. Protoc., 2014, 9, 1771 CrossRef CAS PubMed . H. J. Butler, L. Ashton, B. Bird, G. Cinque, K. Curtis, J. Dorney, K. Esmonde-White, N. J. Fullwood, B. Gardner and P. L. Martin-Hirsch, Nat. Protoc., 2016, 11, 664–687 CrossRef CAS PubMed . H. Barr, O. Old, M. Almond, C. Kendall, G. Rhys-Lloyd, G. Shetty, J. Hutchings and N. Stone, Mater. Today, 2015, 2, 890–893 CrossRef . M. Smolina and E. Goormaghtigh, Analyst, 2018, 143, 2520–2530 RSC . A. Mignolet, V. Mathieu and E. Goormaghtigh, Analyst, 2017, 142, 1244–1257 RSC . K. L. A. Chan and S. G. Kazarian, Chem. Soc. Rev., 2016, 45, 1850–1864 RSC . L. Quaroni, T. Zlateva, B. Sarafimov, H. W. Kreuzer, K. Wehbe, E. L. Hegg and G. Cinque, Biophys. Chem., 2014, 189, 40–48 CrossRef CAS PubMed . I. I. Patel, D. A. Shearer, S. W. Fogarty, N. J. Fullwood, L. Quaroni, F. L. Martin and J. Weisz, Cancer Biol. Ther., 2014, 15, 225–235 CrossRef CAS PubMed . I. Zawlik, E. Kaznowska, J. Cebulski, M. Kolodziej, J. Depciuch, J. Vongsvivut and M. Cholewa, Sci. Rep., 2016, 6, 37333