Using two different thawing temperatures and their effect on the motility recovery of human cryopreserved sperms in cancer patients

Abstract

Human sperm cryopreservation is a widely used technique which helps the male partner for the fertility insurance by preserving their sperms for a long time. Cancer patients suffer from low semen quality especially who undergo chemotherapy or radiation treatments may face complete loss of their sperm production. This study aimed to investigate the effects of different thawing temperature (37 °C & 40 °C) on sperm motility recovery after cryopreservation-involving four types of cancer tumors at variable ages between 16 and 42 years- to help cancer patient for storage their sperms. A detailed semen analysis was collected under guidelines for 30 samples. The samples were then analysed and frozen in liquid nitrogen. The semen samples were subsequently thawed at 37 °C & 40°Cfor 3 min. This was then followed by statistical analysis of the comparative motilities. Results: thawing of cryopreserved human sperm at 40 °C results in a statistically significant increase in motility recovery compared with thawing at 37 °C.