Determination of bioactive markers in Cleome droserifolia using cell-based bioassays for antidiabetic activity and isolation of two novel active compounds
Date
2011
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Urban & Fischer
Series Info
Phytomedicine;19, 38–41
Doi
Scientific Journal Rankings
Abstract
The antidiabetic activities of the aqueous (AqEx) and ethanolic (AlEx) extracts of Cleome droserifolia
(Forssk.) Del., were tested in cultured C2C12 skeletal muscle cells and 3T3-L1 adipocytes. An 18-h treatment with the AqEx increased basal glucose uptake by 33% [insulin equivalent (IE) = 1.3 ± 0.04] in muscle
cells comparable to a 25.5% increase caused by 100 nM insulin (IE = 1 ± 0.03). Fractionation of the tested
AqEx yielded hexane (HxFr), chloroform (ClFr) and ethyl acetate (EtFr) fractions which exerted 38, 52 and
35% increase in the glucose uptake corresponding to an IE of 1.5 ± 0.06, 2.0 ± 0.04 and 1.4 ± 0.04, respectively. Only the ClFr and EtFr accelerated the triglyceride accumulation [rosiglitazone equivalent(RE) was
0.9 ± 0.13 and 0.63 ± 0.12, respectively] in pre-adipocytes undergoing differentiation comparably with
10 M rosiglitazone. Six terpenoids (C1–C6) and three flavonol glycosides (F1–F3) were isolated from the
active ClFr and EtFr, respectively, and identified. C5, C2 and C4 had an IE of 0.86 ± 0.05, 1.01 ± 0.04 and
0.9 ± 0.08, while F1, F2 and F3 gave an IE of 1.3 ± 0.05, 2.3 ± 0.05 and 2.0 ± 0.04, respectively. We could
conclude that the reported antihyperglycemic activity of Cleome droserifolia is attributed to significant
insulin-like effects in peripheral tissues, and that compounds F2 and F3, being highly active, could be
used as bioactive markers to standardize the C. droserifolia herbal extract.
Description
Keywords
droserifolia, Cleome, bioassays, antidiabetic
Citation
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