Molecular characterization of carbapenem-insensitive Acinetobacter baumannii in Egypt

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorAl-Agamy M.H.
dc.contributor.authorKhalaf N.G.
dc.contributor.authorTawfick M.M.
dc.contributor.authorShibl A.M.
dc.contributor.authorEl Kholy A.A.
dc.contributor.otherPharmaceutics and Microbiology Department
dc.contributor.otherCollege of Pharmacy
dc.contributor.otherKing Saud University
dc.contributor.otherPO Box 2457
dc.contributor.otherRiyadh 11451
dc.contributor.otherSaudi Arabia; Microbiology and Immunology Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAl-Azhar University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Microbiology and Immunology Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherModern Arts and Science University
dc.contributor.otherSixth of October City
dc.contributor.otherEgypt; Clinical Pathology Department
dc.contributor.otherFaculty of Medicine
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:42:20Z
dc.date.available2020-01-09T20:42:20Z
dc.date.issued2014
dc.descriptionScopus
dc.description.abstractObjectives: This study investigated the prevalence of diverse Ambler class ?-lactamase-encoding genes in 40 carbapenem-insensitive Acinetobacter baumannii isolates collected from two hospitals in Egypt during the period January-March 2012. Methods: The resistance levels to different groups of antimicrobial agents were determined. PCR was used to detect the different Ambler class ?-lactamases encoding the following genes: blaTEM, blaSHV, blaCTX-M, blaVEB, blaPER, blaGES, blaVIM, blaIMP, blaSIM, blaSPM, blaGIM, blaNDM, blaADC, blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58. ISAba1 and int1 were detected by PCR. Results: The isolates were 100% resistant to amoxicillin-clavulanate, aztreonam, cefepime, cefotaxime, and ceftazidime. Of the isolates, 5% were resistant to colistin, 45% to amikacin, 70% to imipenem, and 85% to ciprofloxacin. The blaADC- and blaOXA-51-like genes were detected in the entire collection. The prevalences of blaOXA-23, blaOXA-24, and blaOXA-58 were 50%, 7.5%, and 5%, respectively. However, the prevalences of blaTEM-, blaPER-, and blaGES-like genes were 87.5%, 55%, and 27.5%, respectively. SHV, CTX-M, VEB, KPC, and MBL encoding genes were not detected. The ISAba1 was found upstream to blaOXA-51, blaOXA-23, and blaADC in 85%, 80%, and 50%, respectively. Of note, 45% (18/40) of the isolates co-produced extended-spectrum ?-lactamases (PER and GES) and carbapenemases (OXA-23 and OXA-58). Conclusions: The blaADC-, blaTEM-, blaPER-, blaOXA-23-, and blaGES-like genes were found to be the most prevalent types of ?-lactamase-encoding gene in A. baumannii collected from Egypt. A high level of carbapenem resistance is mediated by blaOXA-23, blaOXA-24, and blaOXA-58 (minimum inhibitory concentration (MIC) 32 to >256?g/ml), and a low level of carbapenem resistance is mediated by blaGES (MIC 4-16?g/ml) and by up-regulation of ISAba1-OXA-51 (MIC 1-4?g/ml). Class B MBL was not identified to play a role in carbapenem resistance in A. baumannii isolates from Egypt. � 2014 The Authors.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=22380&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1016/j.ijid.2013.12.004
dc.identifier.doiPubMed ID 24607428
dc.identifier.issn12019712
dc.identifier.otherhttps://doi.org/10.1016/j.ijid.2013.12.004
dc.identifier.otherPubMed ID 24607428
dc.identifier.urihttps://t.ly/ge85m
dc.language.isoEnglishen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofseriesInternational Journal of Infectious Diseases
dc.relation.ispartofseries22
dc.subjectAntimicrobial resistanceen_US
dc.subjectCarbapenem-insensitive Acinetobacter baumanniien_US
dc.subjectESBLen_US
dc.subjectPCRen_US
dc.subjectamikacinen_US
dc.subjectamoxicillinen_US
dc.subjectaztreonamen_US
dc.subjectbeta lactamaseen_US
dc.subjectcarbapenemen_US
dc.subjectcarbapenemaseen_US
dc.subjectcefepimeen_US
dc.subjectcefotaximeen_US
dc.subjectceftazidimeen_US
dc.subjectciprofloxacinen_US
dc.subjectclavulanic aciden_US
dc.subjectcolistinen_US
dc.subjectimipenemen_US
dc.subjectantiinfective agenten_US
dc.subjectcarbapenem derivativeen_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectantibiotic resistanceen_US
dc.subjectarticleen_US
dc.subjectbacterial geneen_US
dc.subjectbacterial geneticsen_US
dc.subjectbacterium detectionen_US
dc.subjectbacterium isolateen_US
dc.subjectEgypten_US
dc.subjectgene identificationen_US
dc.subjectminimum inhibitory concentrationen_US
dc.subjectnonhumanen_US
dc.subjectphenotypeen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectupregulationen_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectAcinetobacter Infectionsen_US
dc.subjectantibiotic resistanceen_US
dc.subjectclassificationen_US
dc.subjectenzymologyen_US
dc.subjectepidemiologyen_US
dc.subjectfemaleen_US
dc.subjectgene expressionen_US
dc.subjectgeneticsen_US
dc.subjecthumanen_US
dc.subjectisolation and purificationen_US
dc.subjectmaleen_US
dc.subjectmetabolismen_US
dc.subjectmicrobial sensitivity testen_US
dc.subjectmicrobiologyen_US
dc.subjectmolecular epidemiologyen_US
dc.subjectplasmiden_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectAcinetobacter Infectionsen_US
dc.subjectAnti-Bacterial Agentsen_US
dc.subjectbeta-Lactam Resistanceen_US
dc.subjectbeta-Lactamasesen_US
dc.subjectCarbapenemsen_US
dc.subjectEgypten_US
dc.subjectFemaleen_US
dc.subjectGene Expressionen_US
dc.subjectHumansen_US
dc.subjectMaleen_US
dc.subjectMicrobial Sensitivity Testsen_US
dc.subjectMolecular Epidemiologyen_US
dc.subjectPlasmidsen_US
dc.titleMolecular characterization of carbapenem-insensitive Acinetobacter baumannii in Egypten_US
dc.typeArticleen_US
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