Using Some Micro-elements to Improve Shoot and Root Induction of Date Palm (Phoenix dactylifera L.)

Abstract

This study was developed to improve shoot and root induction in vitro propagation of date palm cv. Sukarri using various micronutrient elements salts (MnSO4,4H2O), (ZnSO4,4H2O) and (CuSO4,7H2O) of different concentrations which added to three media formulation (M1, M2 and M3). Shoots derived from somatic embryos germination were used as explants in the multiplication stage for a period of 30 days of culture. Shoots length, shoot number and leaves number were evaluated and measured. The shoots were separated and sub-cultured to the rooting medium and for 45 days. Same evaluations and measurements were repeated. At the multiplication stage the highest shoots number were formed with the lowest concentration of (MnSO4, 4H2O), (ZnSO4, 4H2O) and (CuSO4, 7H2O) supplemented in M1 medium. However, the rooting stages the highest concentrations of the microelements supplemented in M3 medium were found the best to develop the highest numbers of roots.