A new firewall in the fight against breast cancer: in-vitro and in-silico studies correlating chemistry to apoptotic activity of Otostegia fruticosa

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Date

2022-10

Authors

Al-Madhagy, Somaia. A
Gad, Sameh S
Mostafa, Eman S
Angelonie, Simone
Saad, Muhammed A
Sabry, Omar M
Caprioli, Giovanni
El-Hawary, Seham S

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Journal ISSN

Volume Title

Type

Article

Publisher

Taylor and Francis Ltd.

Series Info

Natural Product Research;2022

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Abstract

Breast cancer is the most devastating disease for women. There is a great demand for new sources to treat this disease. Medicinal plants are an indispensable source of bioactive compounds with wide range of pharmacological activities. In-vitro cytotoxic activity of Otostegia fruticosa methanolic extract against human breast cancer was studied using MCF-7 cell line. The extract showed mildly potent activity (IC50 ¼ 51 ± 9.836 mg/mL) in comparison to the standard anticancer doxorubicin (IC50 ¼ 7.467 ± 1.05 mg/mL). Potential compounds responsible for activity have been identified using Molecular Operating Environment (MOE) module on the major compounds detected by HPLC-MS/MS technique against estrogen alpha receptor (ERaþ: PDB ID 2JF9). 3,5-di-O-dicaffeoyl- quinic acid, hyperoside and rutin showed similar binding and antagonistic interaction with the estrogen alpha receptor as tam- oxifen in several poses. The retrieved results confirm that we can add this plant to a powerful arsenal that combats this insidious disease.

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Keywords

MCF-7, Otostegia fruticosa, HPLC-MS/MS, phenolics, estrogen alpha receptor

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