Phenotypic and molecular characterization of plasmid mediated AmpC ?-lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis isolated from urinary tract infections in Egyptian hospitals

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorHelmy M.M.
dc.contributor.authorWasfi R.
dc.contributor.otherDepartment of Microbiology and Immunology
dc.contributor.otherFaculty of Medicine
dc.contributor.otherZagazig University
dc.contributor.otherZagazig
dc.contributor.otherSharqia
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA)
dc.contributor.other26 July Road Intersection with El Wahat Road
dc.contributor.otherGiza
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:42:12Z
dc.date.available2020-01-09T20:42:12Z
dc.date.issued2014
dc.descriptionScopus
dc.description.abstractThe incidence of resistance by Enterobacteriaceae to ?-lactam/?- lactamase inhibitors combination is increasing in Egypt. Three phenotypic techniques, comprising AmpC disk diffusion and inhibition dependent methods using phenylboronic acid (PBA) and cloxacillin, were compared to PCR based method for detection of plasmid mediated AmpC ?-lactamase in common urinary tract isolates. A total of 143 isolates, including E. coli, Klebsiella pneumonia, and Proteus mirabilis, were collected from urinary tract infections cases in Egyptian hospitals. Plasmid encoded AmpC genes were detected by PCR in 88.46% of cefoxitin resistant isolates. The most prevalent AmpC gene family was CIT including CMY-2, CMY-4, and two CMY-2 variants. The second prevalent gene was DHA-1 which was detected in E. coli and Klebsiella pneumonia. The genes EBC, FOX, and MOX were also detected but in small percentage. Some isolates were identified as having more than one pAmpC gene. The overall sensitivity and specificity of phenotypic tests for detection of AmpC ?-lactamase showed that AmpC disk diffusion and inhibition dependent method by cloxacillin were the most sensitive and the most specific disk tests. PCR remains the gold standard for detection of AmpC ?-lactamases. This study represents the first report of CMY-2 variants of CMY-42 and CMY-102 ?-lactamase-producing E. coli, Klebsiella pneumonia, and Proteus mirabilis isolates in Egypt. � 2014 Mai M. Helmy and Reham Wasfi.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=21100230018&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1155/2014/171548
dc.identifier.doiPubMed ID 25003107
dc.identifier.issn23146133
dc.identifier.otherhttps://doi.org/10.1155/2014/171548
dc.identifier.otherPubMed ID 25003107
dc.identifier.urihttps://t.ly/mb8zB
dc.language.isoEnglishen_US
dc.publisherHindawi Publishing Corporationen_US
dc.relation.ispartofseriesBioMed Research International
dc.relation.ispartofseries2014
dc.subjectbacterial enzymeen_US
dc.subjectbenzeneboronic aciden_US
dc.subjectbeta lactamase AmpCen_US
dc.subjectcefoxitinen_US
dc.subjectcloxacillinen_US
dc.subjectplasmid DNAen_US
dc.subjectAmpC beta-lactamasesen_US
dc.subjectbacterial proteinen_US
dc.subjectbeta lactamaseen_US
dc.subjectantibiotic resistanceen_US
dc.subjectarticleen_US
dc.subjectbacterial geneen_US
dc.subjectbacterium detectionen_US
dc.subjectbacterium isolateen_US
dc.subjectcmy 2 geneen_US
dc.subjectcmy 4 geneen_US
dc.subjectcontrolled studyen_US
dc.subjectdisk diffusionen_US
dc.subjectDNA templateen_US
dc.subjectebc geneen_US
dc.subjectEgypten_US
dc.subjectEscherichia colien_US
dc.subjectEscherichia coli infectionen_US
dc.subjectfox geneen_US
dc.subjectgenetic variabilityen_US
dc.subjectgold standarden_US
dc.subjectKlebsiella oxytocaen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectKlebsiella pneumoniae infectionen_US
dc.subjectmox geneen_US
dc.subjectmultigene familyen_US
dc.subjectnonhumanen_US
dc.subjectphenotypeen_US
dc.subjectplasmiden_US
dc.subjectpolymerase chain reactionen_US
dc.subjectpredictive valueen_US
dc.subjectProteus infectionen_US
dc.subjectProteus mirabilisen_US
dc.subjectProteus vulgarisen_US
dc.subjectsensitivity and specificityen_US
dc.subjecturinary tract infectionen_US
dc.subjectagar gel electrophoresisen_US
dc.subjectenzymologyen_US
dc.subjectEscherichia colien_US
dc.subjectgeneticsen_US
dc.subjectgenotypeen_US
dc.subjecthospitalen_US
dc.subjecthumanen_US
dc.subjectisolation and purificationen_US
dc.subjectKlebsiellaen_US
dc.subjectmicrobial sensitivity testen_US
dc.subjectmicrobiologyen_US
dc.subjectphenotypeen_US
dc.subjectplasmiden_US
dc.subjectProteus mirabilisen_US
dc.subjecturinary tract infectionen_US
dc.subjectBacterial Proteinsen_US
dc.subjectbeta-Lactamasesen_US
dc.subjectEgypten_US
dc.subjectElectrophoresis, Agar Gelen_US
dc.subjectEscherichia colien_US
dc.subjectGenotypeen_US
dc.subjectHospitalsen_US
dc.subjectHumansen_US
dc.subjectKlebsiellaen_US
dc.subjectMicrobial Sensitivity Testsen_US
dc.subjectPhenotypeen_US
dc.subjectPlasmidsen_US
dc.subjectProteus mirabilisen_US
dc.subjectUrinary Tract Infectionsen_US
dc.titlePhenotypic and molecular characterization of plasmid mediated AmpC ?-lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis isolated from urinary tract infections in Egyptian hospitalsen_US
dc.typeArticleen_US
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