Radical scavenging activity and cytotoxicity of Euphorbia hirta L. growing in Egypt

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorBakr R.O.
dc.contributor.authorEl-Raey M.A.E.A.
dc.contributor.authorFarouk C.N.
dc.contributor.authorAhmed H.E.
dc.contributor.authorSaid O.M.E.
dc.contributor.otherPharmacognosy Department
dc.contributor.otherOctober University for Modern Sciences and Arts
dc.contributor.otherGiza
dc.contributor.otherEgypt; Phytochemistry and Plant Chemosystematics Department
dc.contributor.otherNationalResearch Center
dc.contributor.otherDokki
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-25T19:58:28Z
dc.date.available2020-01-25T19:58:28Z
dc.date.issued2012
dc.descriptionScopus
dc.description.abstractEuphorbia hirtaL. (asthma weed, pill bearing spurge),is one of the most important species of Euphorbiaceaeindigenousto Egypt and widely used in folk medicine. It was the subject for many phytochemical and biological studies but correlation between phytoconstituents and biological activities were not well studied.The aim of the present study wasto correlatethe antioxidant and cytotoxic activities against twoof the most important health problems in Egypt with the main constituents in E hirta (phenolic and flavonoid). The different fractions obtained from successive extraction of E hirta were screened for their radical scavenging ability using 1,1 -diphenyl-2-picryl hydrazine (DPPH) testin addition to cytotoxicity using Sulforhodamine B (SRB) assay against liver (Huh-7) and lung (A-549) cell carcinoma. Phenolic and flavonoid contents were estimated using colorimetric assays (Folin-Ciocalteuand aluminum chloride assays respectively).Ethyl acetate (EtOAc) fractionappeared as potent radical scavenger (IC50 5.4�0.65?g/ml and IC90 11.9�0.84?g/ml) and had the highest potency against Huh-7 (IC50 36.7�1.02 ?g/ml)and A-549 (IC50114.4 �0.78 ?g/ml) cancer cell lines. These results are attributed to the highest flavonoid concentration (23�1.06 mg/gquercetin equivalent)in EtOAcfraction in addition to high content of phenolics (88.9 �0.57mg/g gallic acid equivalent). Further studies are necessary to isolate and identify the ethyl acetate's bioactive compounds and evaluation of their biological activities.en_US
dc.identifier.doihttps://doi.org/
dc.identifier.issn9750185
dc.identifier.otherhttps://doi.org/
dc.identifier.urihttps://t.ly/LX9vX
dc.language.isoEnglishen_US
dc.publisherAdvanced Research Journalsen_US
dc.relation.ispartofseriesInternational Journal of Phytomedicine
dc.relation.ispartofseries4
dc.subjectCytotoxicityen_US
dc.subjectDPPHen_US
dc.subjectEuphorbia hirtaen_US
dc.subjectPhenolic contenten_US
dc.subjectSRB assayen_US
dc.subject1,1 diphenyl 2 picrylhydrazylen_US
dc.subjectacetic acid ethyl esteren_US
dc.subjectantineoplastic agenten_US
dc.subjectantioxidanten_US
dc.subjectascorbic aciden_US
dc.subjectbutanolen_US
dc.subjectchloroformen_US
dc.subjectEuphorbia hirta extracten_US
dc.subjectflavonoiden_US
dc.subjectphenol derivativeen_US
dc.subjectplant extracten_US
dc.subjectscavengeren_US
dc.subjectsulforhodamine Ben_US
dc.subjectunclassified drugen_US
dc.subjectwateren_US
dc.subjectantineoplastic activityen_US
dc.subjectantioxidant activityen_US
dc.subjectarticleen_US
dc.subjectcell viabilityen_US
dc.subjectcolorimetryen_US
dc.subjectcontrolled studyen_US
dc.subjectdrug cytotoxicityen_US
dc.subjectdrug effecten_US
dc.subjectdrug mechanismen_US
dc.subjectdrug potencyen_US
dc.subjectEgypten_US
dc.subjectEuphorbia hirtaen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectIC 50en_US
dc.subjectphytochemistryen_US
dc.titleRadical scavenging activity and cytotoxicity of Euphorbia hirta L. growing in Egypten_US
dc.typeArticleen_US
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