Antibacterial, antioxidant, and topical antiinflammatory activities of Bergia ammannioides: A wound-healing plant
Loading...
Date
2015
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Informa Healthcare
Series Info
Pharmaceutical biology;54:2, 215-224
Doi
Scientific Journal Rankings
Abstract
Context: Despite the traditional use of Bergia ammannioides Henye ex Roth. (Elatinaceae) for
the treatment of wounds in India, there is a scarcity of scientific data supporting this use.
Objective: The objective of this study is to assess wound-healing potentiality of the plant,
to study pharmacological activities that may contribute in eliminating wound complications,
and to investigate the biologically active fractions.
Material and methods: The ethanolic extract (EtOH) of the aerial parts was fractionated to
obtain n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuOH) fractions.
EtOH and its fractions were formulated in strength of 5 and 10% w/w ointment and tested
for wound-healing activity using the excision model. The topical anti-inflammatory, in vitro
antioxidant, and antibacterial activities were evaluated. HxFr and EtFr were chemically
investigated to isolate their constituents.
Results: Application of EtOH, HxFr, and EtFr (10% w/w ointments) leads to 71.77, 85.62,
and 81.29% healing of the wounds with an increase in the collagen content. HxFr had the
strongest anti-inflammatory (64.5% potency relative to Voltaren) and antibacterial activity
(MIC ¼ 104 mg/ml against Staphylococcus aureus), while EtFr showed the strongest antioxidant
activity against DPPH, ABTS+
, and super oxide radical with an IC50 value of 10.25 ± 0.01,
66.09 ± 0.76, and 167.33 ± 0.91 mg/ml, respectively. b-Sitosterol, lupeol, cyclolaudenol, and
cycloartenol were isolated from HxFr. Quercetin, ellagic acid, kaempferol-3-O-a-L-rhamnoside,
and quercetin-3-O-a-L-rhamnoside were isolated from EtFr.
Discussion and conclusion: Our study presents scientific evidence for the efficacy of
B. ammannioides in enhancing wound healing, and the first isolation of cyclolaudenol and
cycloartenol from Bergia
Description
MSA Google Scholar
Keywords
Cycloartenol, cyclolaudenol, wound complications
Citation
Aliyeva E, Umur S, Zafer E, Acigoz G. (2004). The effect of polylactide membranes on the levels of reactive oxygen species in periodontal flaps during wound healing. Biomaterials 25:4633–7. Anandjiwala S, Srinivasa H, Kalola J, Rajani M. (2007a). Free-radical scavenging activity of Bergia suffruticosa (Delile) Fenzl. Nat Med 6: 59–62. Anandjiwala S, Srinivasa H, Rajani M. (2007b). Isolation and TLC Densitometric quantification of gallicin, gallic acid, lupeol and b-sitosterol from Bergia suffruticosa, a hitherto unexpected plant. Chromatographia 66:725–34. Atta AH, Alkohafi A. (1998). Anti-nociceptive and anti-inflammatory effects of some Jordanian medicinal plants extracts. J Ethnopharmacol 60:117–24. Baboir BM. (1978). Oxygen dependent microbial killing by phagocytes (first of two parts). N Eng J Med 298:629–68. British Pharmacopoiea. (1996). Simple Ointment. London: HMSO, 1360. Buffoni F, Bancheli G, Cambi S, et al. (1993). Skin wound healing: Some biochemical parameters in Guinea pig. J Pharmaceutics Pharmacol 45:784–90. Clark RAF. (1996). Wound repair: Overview and general consideration. In: Clark RA, Henson PM, eds. Molecular and Cellular Biology of Wound Repair. New York: The Plenum Press, 112–13. Davis CC, Chase MW. (2004). Elatinaceae are sister to Malpighiaceae; Peridiscaceae belong to Saxifragales. Am J Bot 91:262–73. Delazar A, Byres M, Gibbons S, et al. (2004). Iridoid glycosides from Eremostachys glabra. J Nat Prod 67:1584–7. Elizabeth K, Rao MNA. (1990). Oxygen radical scavenging activity of curcumin. Int J Pharm 58:237–40. Fleischer AB, Feldman SR, White RE, et al. (1997). Procedures for skin diseases performed by physicians in 1993 and 1994: Analysis of data from the national ambulatory medical care survey. J Am Acad Dermatol 37:719–24. Glynn LE. (1981). The pathology of scar tissue formation. In: Glynn LE, ed. Handbook of Inflammation, vol. 3. Tissue Repair and Regeneration. Amsterdam: Elsevier/North Holland Biomedical Press, 34–5. Goad J, Akihisa, T. (1997). Analysis of Sterols, 1st edn. London: Blackie Academic and Professional Press, Champan and Hall. Gomathi K, Gopinath D, Rafiuddin AM, Jayakumar R. (2003). Quercetin incorporated collagen matrices for dermal wound healing processes in rats. Biomaterials 24:2767–72. Heinrich M, Gibbons S. (2001). Ethnopharmacology in drug discovery: An analysis of its role and potential contribution. J Pharmacy Pharmacol 53:425–32. Hoskeri YH, Krishna V, Jignesh S, et al. (2012). In-silico drug designing using b-sitosterol isolated from Flaveria trinervia against peptide deformylase protein to hypothesize bactericidal effect. Int J Pharmacy Pharm Sci 4:192–6. Jewo PI, Fadeyibi IO, Babalola OS, et al. (2009). A comparative study of the wound healing properties of moist exposed burn ointment (Mebo) and silver sulphadiazine. Ann Burns Fire Disasters XXII: 79–82. Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. (2007). Ethnopharmacological approaches to wound healing – Exploring medicinal plants of India. J Ethnopharmacol 114:103–13. Mabry TJ, Markham KR, Thomas MB. (1970). The Systematic Identication of Flavonoids. Berlin: Springer-Verlag. Manivannan R, Prabakaran K, Ilayaraja S. (2014). Isolation, identification and antibacterial and wound healing studies of quercetin-3-o-a-Lrhamnopyranoside-200-gallate. Int J Appl Sci Eng 12:99–106. Markham KR. (1982). Techniques of Flavonoid Identification. London: Academic Press. Majumdar MR, Kamath JV. (2005). Herbal concept on wound healing. J Pharmaceut Res 4:01–07. Narendhirakannan RT, Nirmala JG, Caroline A, et al. (2012). Evaluation of antibacterial, antioxidant and wound healing properties of seven traditional medicinal plants from India in experimental animals. Asian Pac J Trop Biomed 2:S1245–53. NCCLS (National Committee for Clinical Laboratory Standards). (2000). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically – 5th edn. Approved Standard M7-A5. Wayne (PA): NCCLS. Ozgen M, Reese RN, Tulio AZ, et al. (2006). Modified 2,2-azino-bis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,20 -diphenyl-1- picrylhydrazyl (DPPH) methods. J Agric Food Chem 54:1151–7. Okoli CO, Akah PA, Ezugworie U. (2006). Anti-inflammatory activity of extracts of root bark of Securidaca longipedunculata Fres (Polygalaceae). Afr J Trad Compl Alter Med 3:54–63. O¨ ksu¨z S, Ulubelen A, Barla A. (2002). Terpenoids and aromatic compounds from Euphorbia heteradena. Turk J Chem 26:457–63. Parekh J, Nair R, Chanda S. (2005). Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity. Ind J Pharmacol 37:408–9. Saleem M. (2009). Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett 285:109–15. Takao T, Watanabe N, Yagi I, Sakata K. (1994). A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci Biotech Biochem 58: 1780–3. Thiem B, Grosslinka O. (2003). Antimicrobial activity of Rubus chamaemorus leaves. Fitoter 75:93–5. Ur-Rehman A, Siddiqa A, Abbasi MA, et al. (2013). Bergia ammannioides: Phytochemical screening, antioxidant activity and radical scavenging effects of its various fractions. Asian J Chem 25: 7921–6. Yan XH, Guo YW. (2004). Two newellagic acid glycosides from leaves of Diplopanax stachyaanthus. J Asian Nat Prod Res 6:271–6. Woessner JF. (1961). The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys 93:440–7.