Adiponectin (+276 G/T), Tumor Necrosis Factor-alpha (-308 G/A) and Interleukin-6 (-174 C/G) Genes Polymorphisms in Egyptian Type 2 Diabetic Patients
Date
2016-05
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
OMICS INT PVT LTD
Series Info
JOURNAL OF DIABETES & METABOLISM;Volume: 7 Issue: 5
Scientific Journal Rankings
Abstract
Type 2 diabetes mellitus (T2DM) is a metabolic pro-inflammatory disorder characterized by chronic hyperglycemia and increased levels of circulating cytokines. Adiponectin, tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (Il-6), are important cytokines mediators in the pathogenesis of T2DM. The single nucleotide polymorphisms (SNPs) present in the regulatory regions of cytokine genes often have an impact on their expression levels.
Aim: Explore potential associations between SNP+276 G/T of adiponectin, SNP -308 G/A of TNF-alpha and SNP -174 C/G of IL-6 genes with T2DM and to assess its influence on their serum levels.
Subjects and Methods: From the Egyptian population, we enrolled 95 T2DM patients and 85 non-diabetic controls. Serum adiponectin, TNF-alpha and IL-6 were measured. Genotyping for three SNPs of the adiponectin, TNF-alpha and IL-6 genes was performed by polymerase chain reaction-restriction fragment length polymorphism.
Results: Subjects with the GT/TT genotype of SNP 276 were at increased risk for T2DM (OR=15.88, CI=7.56-33.31, P = 0.01) and associated with hypoadiponectinemia compared with the GG genotype. Furthermore, the allelic frequency of the A allele of SNP 308 was significantly different between T2DM patients compared to controls (X2(=)30.54, P = 0.0001). Moreover, Individuals with T2DM carrying the GA/AA genotypes had significantly higher serum TNF-alpha levels than those carrying GG genotype. In addition, Carriers of G allele of IL-6 were significantly more likely associated with T2DM.
Conclusion: Genetics variations in Adiponectin +276 G/T, TNF-alpha 308 G/A and IL-6 -174 C/G may contribute to the disposition for T2DM in Egyptian patients.
Description
Accession Number: WOS:000384466400007
Keywords
RISK, ASSOCIATION, APM1 GENE, JAPANESE PATIENTS, METABOLIC SYNDROME, ENERGY-EXPENDITURE, PROMOTER POLYMORPHISM, INSULIN-RESISTANCE, CORONARY-ARTERY-DISEASE, IMPAIRED GLUCOSE-TOLERANCE, Interleukin-6-174 C/G, Tumor necrosis factor-alpha 308 G/A, Adiponectin +276 G/T, Polymerase chain reaction-restriction fragment length polymorphism, Single nucleotide polymorphism
Citation
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