Effect of Coconut Milk, Casein Hydrolysate and Yeast Extract on the Proliferation of in vitro Barhi Date Palm (Phoenix dactylifera L.)

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorM. Hosny, Shereen
dc.contributor.authorHammad, Gehan
dc.contributor.authorEl Sharbasy, Sherif
dc.contributor.authorZayed, Zeinab
dc.date.accessioned2020-02-29T12:01:18Z
dc.date.available2020-02-29T12:01:18Z
dc.date.issued2016
dc.descriptionMSA Google Scholaren_US
dc.description.abstractThis research was aimed at the enhancement of shoot regeneration via the supplementation of natural compounds on Date Palm cv. Barhi. Small clusters consisting of (2-3 shoots) were used as explants and cultured on MS medium supplemented with three types of natural additives at three different concentrations: (10%, 20%, 30% for Coconut Milk and1.25g/l, 2.5g/l, 5.0g/l for Casein Hydrolysate and Yeast Extract), in addition to the control (0.05 BA mg/l, 0.1 NAA mg/l). Results showed the most efficient secondary somatic embryo formation in association with casein hydrolysate 5.00g/l and that for the growth vigor, coconut milk was the most effective component. The Yeast Extract produced the lowest readings in all assessed concentrations. Chemical analysis was also performed that tested Chlorophyll ‘A’ and ‘B’, total Carbohydrate, Protein, Amino Acid, Phenol and Indole. Coconut Milk 30% and Casein Hydrolysate 2.5g/l gave the best overall results, both in test responsiveness and regenerative abilities.en_US
dc.identifier.citationKhierallah, H.S.M. and N.H. Hussein, 2013. The Role in selected bulbous ornamentals: BAP as of coconut water and casein hydrolysate in somatic Inducer, In: Mujib, A. and Samaj, J. (eds.): Somatic embryogenesis of Date Palm and genetic stability Embryogenesis, Springer-Verlag, Berlin Heidelberg, detection using RAPD markers. Date Palm Research 15-24. Unit, College of Agriculture, University of 12. Lichtenthaler, H.K. and A.R. Wellburn, 1985. Baghdad, Iraq. Determination of total carotinoids and chlorophyll A 2. Jain, S.M., J.M. Al-Khayri and D.V. Johnson, 2011. and B of leaf in Different Solvents. Biol. Soc. Trans., Date Palm Biotechnology” Springer Dordrecht 11: 591-592. Heidelberg London New York, Department of 13. Bradford, M.M., 1976. A Dye Binding Assay for Agricultural Sciences, University of Helsinki, Protein. Anal. Biochem., 72: 248-254. Finland. 14. Dubois, M., F. Smith, K.A. Gilles, J.K. Hamilton 3. Zaid, A. and P.F. De Wet, 2002. Date Palm and P.A. Rebers, 1956. Colorimetric Method for Propagation, in: Zaid, A., ed., Date Palm Cultivation, Determination of Sugars and Related Substances. FAO Plant Production and Protection Paper no. 156, Annal. Chem., 28(3): 350-356. Food and Agriculture Organization of the United 15. Bates, L.S., R.P. Waldern and I.D. Tear, 1973. Nations, Rome, pp: 29-44. Rapid determination of free proline under water 4. Al Khayri, J.M., 2010. Somatic embryogenesis of stress studies. Plants and Soil, 39: 205-207. Date Palm Improved by coconut water. Department 16. A.O.A.C., 1980. Association of Official Agriculture of Agriculture Biotechnology, College of Agriculture Chemists Official Method of Analysis, Washington, Food Science, King Faisal University, Al Hassa, D.C., U.S.A. Saudi Arabia. 17. Larsen, P., A. Harbo, S. Klungroun and T. Ashein, 5. Beshir, I., S. El Sharbassy, G. Safwat and A. Diab, 1962. On the biogenesis of some Indole compounds, 2012. The effects of some natural materials in the in: Acetobacter xylinum. Physiol Plant., 15: 552-565. development of shoot and root of Banana (Musa 18. Smith, P.K., R.L. Krohn, G.T. Hermanson, A.K. Mallia, spp.) using tissue culture technology. New York F.H. Gartner, W. Snedekor and W. Cochran, 1980. Science Journal, 5(1): 132-138. Statistical Methods, 7 ed., Iowa State Univ. Press, 6. George, F.E., 1993. Plant tissue Culture Ames, Iowa, USA. Technique, The Components of Culture Media, 19. Tisserat, B., 1979. Propagation of Date Palm Printed in the UK by Butler and Tanner Ltd, Frome (Phoenix Dactylifera L.) in vitro. J.of Exp. Botany, Somerset, 273. 30:119: 1275-1283. 7. El-Khateeb, A.A., 2008. Comparison effects of 20. Bhaskaran, S. and R.H. Smith, 1992. Somatic sucrose and Date Palm syrup on somatic embryogenesis from shoot tip and immature embryogenesis of Date Palms Phoenix Dactylifera inflorescence of (Phoenix dactylifera L.) CV Barhee. L., Am. J. Biotech Biochem, 4(1): 9-23. Plant Cell Rep., 12: 22-25. 8. El Assar, A.M., W.M. El Messeih and M.R. El- 21. Phillips, R.L., S.M. Kaeppler and P. Olhoft, 1994. Shenawi, 2004. Applying some natural extracts and Genetic instability of plant tissue culture: growth regulators to culture media and their effect on Breakdown of Normal Control. Proceeding of the Sewi cv. Date Palm tissue growth in vitro. Assiut National Academy of Science USA. 91: 5222-5226. Journal of Agricultural Science, 34(4): 155-168. 22. Cullis, C.A., 1999. Environmental stress- A generator 9. Murashige, T. and F.A. Skoog, 1962. A Revised of adaptive variation, In: Lerner, H.R. (Ed). Plant medium for rapid growth and bioassays with Adaptation to Stress Environments’, Marcel Dekker, Tobacco tissue cultures. Physiology Planterum, New York, pp: 149-160. 15: 473-497. 23. Klekowski, E.J., 1985. Mutations, apical cells and 10. Pottino, B.G., 1981. Methods in Plant Tissue Culture, vegetative reproduction. Proceedings of the Royal Department of Horticulture, Agricultural College, Society of Edinburgh. Section B. Biological Sciences Maryland Univ. College Park, Maryland, USA, 8-29. pp: 67-73. 24. McCubbin, M.J., A. Zaid and J. Van Staden, 2004. 30. Neumann, K.H., A. Kumar and J. Imani, 2009. Plant A southern african survcy conducted for off- cell and tissue culture – A Tool in biotechnology, types on date palm produced using somatic basics and applications. Springer-Verlag, Berlin embryogenesis. Emir.J.Agric.Sci., 16(1): 8-14. Heidelberg. 25. Al-Kaabi, H.H., A. Zaid, H. Shephard and 31. Thorpe, T.A., C. Stasolla, E.C. Yeung, G.J. de Klerk, C. Ainsworth, 2007. AFLP variation in tissue A. Roberts and E.F. George, 2008. The Components culture-derived Date Palm (Phoenix Dactylifera L.) of Plant Tissue Culture Media II: Organic Additions, plants. Acta Hort., 736: 135-159. Osmotic and pH Effects and Support Systems, In: 26. Duhamet, L. and R.G. Gautheret, 1950. Structure George, E.F., Hall M.A., de Klerk G-J., eds., Plant Anatomique de Fragments de Tubercules de Propagation by Tissue Culture, 3 . ed., Vol. 1, The Topinambour Cultives en Presence de Lait de Coco. Background, Springer-Verlag, Dordrecht, pp: 115-173. Comp. Rendus de la Soc. de Biol., 144: 177-184. 32. Nasib, A., K. Ali and S. Khan, 2008. An Optimized 27. Baque, A., Y.K. Shin, T. Elshmari, E.J. Lee and and improved method for the in vitro propagation of K.Y. Pack, 2011. Effect of light quality, sucrose Kiwifruit (Actinidia deliciosa) using coconut water. and coconut water concentration on the Pak. J. Bot., 40: 2355-2360. micropropagation of canthe hybrids. Australian 33. Sudipta, K.M., M. Kumara Swamy and M. Anuradha, Journal of Crop Science, 5(10): 1247-1254. 2013. Influence of Various Carbon Sources and 28. Pelegrini, L.L., L.L. Ribas, E. Amano and M. Qoirin, Organic Additives on In vitro Growth 2013. Somatic embryogenesis and morphoanatomy of andMorphogenesis of Leptadenia reticulata Ocotea porosa somatic embryos. Ciencia Florestal, (Wight & Arn), A Valuable Medicinal Plant of India. Sanata Maria, 24(4): 595-605. Int. J. Pharm. Sci. Rev. Res., 21(2), Jul – Aug 2013; 29. Abraham, F., A. Bhatt, C.L. Keng, G. Indrayanto and n32, 174-179. S. Sulaiman, 2011. Effect of yeast extract and chitosan on shoot proliferation, morphology and antioxidant activity of curcuma mangga in vitro plantlets. African Journal of Biotechnology, 10(40): 7787-7795.en_US
dc.identifier.doihttps://doi.org/10.5829/idosi.jhsop.2016.8.1.1172
dc.identifier.issn2079-2158
dc.identifier.otherhttps://doi.org/10.5829/idosi.jhsop.2016.8.1.1172
dc.identifier.urihttps://t.ly/XA7Ze
dc.language.isoenen_US
dc.publisherJournal of Horticultural Science & Ornamental Plantsen_US
dc.relation.ispartofseriesJournal of Horticultural Science & Ornamental Plants;8 (1): 46-54, 2016
dc.subjectPhoenix dactylifera Len_US
dc.subjectCoconut Milken_US
dc.subjectCasein Hydrolysateen_US
dc.subjectYeast extracten_US
dc.subjectProliferationen_US
dc.subjectSomatic embryogenesisen_US
dc.titleEffect of Coconut Milk, Casein Hydrolysate and Yeast Extract on the Proliferation of in vitro Barhi Date Palm (Phoenix dactylifera L.)en_US
dc.typeArticleen_US

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