Phytochemical and pharmacological screening of seeds and fruits pulp of Cucurbita moschata duchesne cultivated in Egypt
Date
2014
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
International Journal of Pharmacognosy and Phytochemistry
Series Info
International Journal of Pharmacognosy and Phytochemistry;Volume: 29 Issue: 1 Pages: 1226-1236
Doi
Scientific Journal Rankings
Abstract
Family Cucurbitaceae is one of the most important
families known for its edible fruits. It was, therefore,
deemed of interest to carry out phytochemical and
pharmacological studies on one of its species which is
Cucurbita moschata Duchesne as one of widely used
edible plants. Phytochemical screening of its seeds and
fruits pulp revealed the presence of carbohydrates,
saponins and steroids in considerable amounts in both of
them. GLC analysis for lipoidal content of seeds revealed
that lauric acid was the major saturated fatty acid while
oleic and linoleic acids were the major unsaturated fatty
acids. Vitamin A and E were estimated using RP-HPLC
analysis. The highest vitamin E concentration was found
in the petroleum ether extract (PEE) of the seeds while the
highest vitamin A concentration was observed in 70%
aqueous methanolic extract (AME) of seeds.
Pharmacological investigation revealed that, AME of
Cucurbita moschata Duchesne seeds and fruits pulp have
favorable anti-inflammatory activities without notable
ulcerogenic effect; that usually associated the antiinflammatory drugs; based on their antioxidant properties.
Moreover, there are remarkable analgesic and
antidepressant activities recorded for AME of its seeds.
Description
MSA Google Scholar
Keywords
University of Cucurbita moschata Duchesne, lipid profile, vitamins, analgesic, anti-inflammatory, antidepressant.
Citation
[1] El-Zalabani, S. (1997): A pharmacognostical study of certain plants belonging to family cucurbitaceae, PHD Thesis, Cairo University, Faculty of Pharmacy, Pharmacognosy Department. [2] Rahman, M., Anisuzzaman, M., Ahmed, F., Rafiul, A. and Naderuzzaman, A. (2008): Study of nutritive value and medicinal uses of cultivated Cucurbits. Journal of Applied Science Research 4 555-558. [3] Dhiman, K., Gupta, A., Sharma, D.K.,Gill N.S. and Goyal, A. (2012): A Review on the Medicinally Important Plants of the Family Cucurbitaceae. Asian Journal of Clinical Nutrition, 4: 16-26. [4] Whitaker, T.W. and Davis, G.N. (1962): Cucurbits: botany, cultivation and utilization. New York: Interscience Publisher Inc., 19621103208, p27. [5] Whitaker, T.W. (1947): American origin of cultivated cucurbits. Annals of the Missouri Botanical Garden, 34: 101–111. [6] Whitaker, T.W. (1956): The origin of the cultivated cucurbita. The American Naturalist, 90 (852): 171– 176. [7] Balkaya, A., Özbakir, M. and Kurtar, E.S. (2010) :The phenotypic diversity and fruit characterization International Journal of Pharmacognosy and Phytochemistry, ISSN: 2051-7858, Vol.29, Issue.1 1234 © RECENT SCIENCE PUBLICATIONS ARCHIVES |March 2014|$25.00 | 27703371| *This article is authorized for use only by Recent Science Journal Authors, Subscribers and Partnering Institutions* of winter squash (Cucurbita maxima) populations from the Black Sea Region of Turkey, African Journal of Biotechnology,9(2):152-62. [8] Susan, D. and Arnum ,A .(1998) : Vitamin A in Kirk- Othmer Encyclopedia of Chemical Technology. New York: John Wiley. pp. 99–107. [9] Ajuru, M.G. and Okoli, B.E. (2013):The Morphological Characterization of the Melon Species in the Family Cucurbitaceae Juss. and their Utilization in Nigeria. International Journal of Modern Botany 3(2):15-19. [10] Yoshinari, O., Sato, H. and Igarashi, K. (2009):"Antidiabetic effects of pumpkin and its components, trigonelline and nicotinic acid, on Goto-Kakizaki rats". Biosci. Biotechnol. Biochem.73 (5): 1033-41. [11] Juknevičienė, E., Černiauskienė, J., Kulaitienė, J. and Juknevičienė, Ž. (2013): Oil pumpkins – Important source of antioxidants Journal of Food, Agriculture & Environment 11 (1): 156-158. [12] Tsai, Y.S., Tong, Y.C., Cheng, J.T., Lee, C.H., Yang, F.S. and Lee, H.Y.( 2006): Pumpkin seed oil and phytosterol F can block testosterone prazosin induced prostate growth in rats. Urologia internationalis 77(3):269-74. [13] Krimer-Malesevic, V., Madarev-Popovic, S., Vastag, Z., Radulovic, L. and Pericin, D. (2011) :Nuts and Seeds in Health and Disease Prevention. (Preedy VR, Watson RR and Patel VB, editors) Academic Press, ISBN: 978-0-12-375688-6: 235-238. [14] Anonymous (2000): Official Methods of Analysis, 7 th Ed Association of Official Analytical Chemists, AOAC, Arlington, V. A. [15] Sofowara, A. (1993): Medicinal plants and Traditional medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria. P289. [16] Trease, G.E., Evans, W.C. (1989): Pharmacognsy, 11thedn. Brailliar TiridelCan. Macmillian publishers. [17] Harborne, J.B. (1973): Phytochemical methods, London. Chapman and Hall, Ltd. pp. 49-188. [18] Stöggl ,W., Huck, C., Wongyai, S., Scherz, H. and Bonn, G. (2005): Simultaneous determination of carotenoids, tocopherols, and γ-oryzanol in crude rice bran oil by liquid chromatography coupled to diode array and mass spectrometric detection employing silica C30 stationary phases. Journal of Separation Science 28 (14): 1721-1718. [19] Finar, I.L. (1973): "Organic Chemistry", 6th Ed., Longman group Ltd., England, p 445. [20] Vogel, A. j. (1975):"A text book of practical organic chemistry". 3rd Ed., Longman Group Ltd. London. P 969-971. [21] Turner, R.A.(1965): Screening Methods In Pharmacology. Academic Press Inc, London, p. 104-157. [22] AL-Rahawi, K., AL-Kaf, A., Yassin, S., EL-Nabtity, S. and EL-Sayed, K. (2013): Synthesis and Biological Activities of 2- Carboxyphenyloxamoylamino Acids, their Salts with 2-ethoxy-6,9-Diaminoacridine and Dglucosamine. Adv Pharmacoepidem Drug Safety2, 2: 129-135. [23] Patil, S.D. and Fursule, R.A. (2008): Effect of Phaseolous trilobus seeds on the paracetamol induced liver damage in rats. J. of Pharmacology and toxicology 3 (7): 485-492. [24] Rakesh, D., Jagdish, S. and Gupta, R. (2010): Potentiative activity of Cucurbita moscata seed extract with beta vulgaris & smilax regelii root extract to reduce extra fats from the body. IJPSR Vol. 1, Issue 2 56-62 [25] Winter, E., Rilsley, G. and Nuss,W. (1962) Proc. Soc. Exp. Biol.Med.544 [26] Wilhelmi, R. and Menasse,G. (1972): Pharmacology 8: 321-328. [27] Malaya, G., Upal, K., Ramanathan,S. and Thangavel, S. (2003): Studies on Anti-inflammatory, Analgesic and Antipyretic Properties of Methanol Extract of C. bonducella leaves in Experimental Animal Models. Iranian Journal Of Pharmacology & Therapeutics, 2:30-34. [28] Pauls, A., Wick, N., Mackay, E.M. (1974): Gastroentrology 8; 774-782. [29] Porsolt, R. D., Anton, G., Blanet, N. andJalfre, M.( 1978) Eur. J. Pharmacol.47, 379. [30] Vincent, C., Paul ,M., Sylvain, R., and Roger ,D. ( 2010): Rodent Models of Depression: Forced Swim and Tail Suspension Behavioral Despair Tests in Rats and Mice. Current Protocols in Pharmacology 5.8.1-5.8.14 [31] Hania, I. A., Soliman, S., Rasha, I.A., Laila, A.E., Wael, B. G. and Sanjiv D. (2011): Erythropoietin protects against doxorubicininduced heart failure: Am J Physiol Heart Circ Physiol, 301:(6) H2413- H2421. [32] Satoh, K.(1987): Lipid peroxide (Malondialdehyde) coloremetric Methods. Clin. Chem. Acta, 90: 37 [33] Packer, L. and Glazer, A. N. (1990): method in enzymology .vol. 186 part B, Academic press Inc. new York, PP. 251 [34] Sinha, A. K. (1972): colorimetric assay of catalase. Analytical biochemistry, 47,389. [35] Green, G.A. (2001) : Understanding NSAIDs: from aspirin to COX-2. Clinical cornerstone. 3(5): 50– 60. International Journal of Pharmacognosy and Phytochemistry, ISSN: 2051-7858, Vol.29, Issue.1 1235 © RECENT SCIENCE PUBLICATIONS ARCHIVES |March 2014|$25.00 | 27703371| *This article is authorized for use only by Recent Science Journal Authors, Subscribers and Partnering Institutions* [36] Goodwin, J.S. and Brodwick, M.(1995) : Diet, aging and cancer.Clin.Geriatr.Med.,11:577-589. [37] Stahelin,H.B.,Gey,K.F.,Eichholzer,M.,Ludin,F.B.,T hurneys,J. and Brubacher,G. (1991): Plasma antioxidant vitamins and subsequent cancer mortality in the 12- year follow up of the prospective basal study.Am.J.Epidemiol.,133:766- 775. [38] Stemiz, A. and Sen, A. (2007): Antioxidant and chemoprotective properties of Momordi cacharantia L. (bitter melon) fruit extract.Afr.J.Biotechnol.,6: 273-277. [39] Kumar, S., Kumar, D.,Saroha, K., Singh, N. and Vashishta, B. (2008): Antioxid and free radical scavenging potential of Citrullus colocynthis (L) Schard.methanolic fruit extract. Acta Pharma., 58:215-220. [40] Marzouka,B.,Marzoukb,Z.,Halouib,E.,Feninab,N.,B ouraouic,A. and Aounia ,M. (2010): Screening of analgesic and anti-inflammatory activities of Citrullus colocynthis from Southern Tunisia. J.Ethnopharmacol., 128:15-19. [41] Hayouni ,E.A., Abedrabba, M. ,Bounix,M. and Hamdi,M. (2007) : The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem., 105: 1126-1134. [42] Eromosele, C.O. and Eromosele, I.C. (2002): Fatty acid compositions of seed oils of Haematostaphis barteri and Ximenia americana. Bioresource Techn 82: 303–304. [43] Al-Jassir, M.S., Mustafa, A.I., Nawawy, M.A. (1995): Studies on seeds (Mesembryanthemum forsskalei Houchst) growing in Saudi Arabia 2: Chemical composition and microflora of Samh seeds.Plants Food Hum Nutr 48: 185–192. [44] Kim, M.Y., Kim, E.J., Kim, Y.N., Choi, C. and Lee B.H. (2012): Comparison of the chemical compositions and nutritive values of various pumpkin (Cucurbitaceae) species and parts. Nutr Res Pract 6(1):21-27. [45] Craig, H., Susan, H. and Joan, M. (2007): Proteinsource tryptophan as an efficacious treatment for social anxiety disorder. Canadian Journal of Physiology and Pharmacology, 85(9): 928-932 [46] El-Shenawy, S.M., Abdel-Salam, O.M., Baiuomy, A.R., El-Batran, S. and Arbid, M.S. (2002): Studies on the Anti-inflammatory and anti-nociceptive effects of melatonin in the rat. Pharmacol Res 46: 235-243. [47] Gill; N., Dhiman, K., Bajwa, J., Sharma , P. and Sood, S. (2010): Evaluation of Free Radical Scavenging, Anti-inflammatory and Analgesic Potential of Benincasa hispida Seed Extract. International Journal of Pharmacology, 6: 652-657. [48] Zhang, X., Wang, H., Song, Y., Nie, L., Wang, L., Liu, B., Shen, P. and Liu Y. (2006): Isolation, structure elucidation, antioxidative properties of two novel dihydrocoumarins for Aloe vera. Bio org. Med.Chem. Lett. 16: 949-953. [49] Cuman, R.K.N., Bersani-Amadio C.A. and Fortes Z.B. (2001): Influence of type 2 diabetes on the inflammatory response in rat. Inflamm Res.,50: 460- 465. [50] Carrillo, C., Cavia-Mdel, M., Alonso-Torre, S. (2012): Role of oleic acid in immune system; mechanism of action; a review. Nutr Hosp., 27(4):978-90. [51] Franson, R., Raghupathi ,R., Fry, M., Saal, J., Vishwanath, B. and Ghosh, S.S. (1990): Inhibition of human phospholipases A2 by cis-unsaturated fatty acids and oligomers of prostaglandin B1. Adv Exp Med Biol, 279: 219-30. [52] Alaoui El Azher, M., Havet, N., Singer, M., Dumarey, C.andTouqui, L.( 2000) :Inhibition by unsaturated fatty acids of type II secretory phospholipase A2 synthesis in guinea-pig alveolar macrophages evidence for the eicosanoidindependent pathway. Eur J Biochem; 267 (12): 3633-9. [53] Koblyakov,V.A.(2001): Free radicals and inflammation (progress in inflammation research series ). Biochemistry, 66 : 937-938. [54] Marianna, N.,Xanthopoulou, T., Elizabeth, F., Smaragdi; A. (2009): Antioxidant and lipoxygenase inhibitory activities of pumpkin seed extracts .Food Research International ;1: 300-12 [55] Murkovic, M., Piironen, V., Lampi, V., Kraushofer, T. and Sontag, G. (2004): Changes in chemical composition of pumpkin seeds during the roasting process for production of pumpkin seed oil (Part 1: non-volatile compounds). Food Chemistry, 84: 359–365. [56] Bavec, F., Grobelnik Mlakar, S., Rozman, C., and Bavec, M. (2007):"Oil Pumpkins: Niche for Organic Producers". Issues in new crops and new uses. Purdue University Agriculture, Horticulture and Landscape Architecture: 185-189. [57] Woodward, W.R., Olanow, C.W.,Beckner,R.M. , Hauser, R.A. , Gauger, L.L. ,Cedarbaum, J.M. and Nutt, J.G. (1993): The effect of L-dopa infusions with and without phenylalanine challenges in parkinsonian patients: Plasma and ventricular CSF L-dopa levels and clinical responses. Neurol., 43:1704-1708. [58] Craig, H., Susan, H., Hecht, T. and Joan, M.(2005): Protein source tryptophan versus pharmaceutical International Journal of Pharmacognosy and Phytochemistry, ISSN: 2051-7858, Vol.29, Issue.1 1236 © RECENT SCIENCE PUBLICATIONS ARCHIVES |March 2014|$25.00 | 27703371| *This article is authorized for use only by Recent Science Journal Authors, Subscribers and Partnering Institutions* grade tryptophan as an efficacious treatment for chronic insomnia. Nutr Neurosci., 8(2):121-127. [59] Al-Zuhair, H., Abdel Fattah, A.A. and Ibrahim, M. (2000): Pumpkin-seed oil modulates the effect of felodipine and captopril in spontaneously