Antimicrobial resistance pattern and their beta-lactamase encoding genes among Pseudomonas aeruginosa strains isolated from cancer patients

Simple item page
dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorZafer M.M.
dc.contributor.authorAl-Agamy M.H.
dc.contributor.authorEl-Mahallawy H.A.
dc.contributor.authorAmin M.A.
dc.contributor.authorAshour M.S.E.-D.
dc.contributor.otherDepartment of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAhram Canadian University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Pharmaceutics and Microbiology
dc.contributor.otherCollege of Pharmacy
dc.contributor.otherKing Saud University
dc.contributor.otherP.O. Box 2457
dc.contributor.otherRiyadh 11451
dc.contributor.otherSaudi Arabia; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAl-Azhar University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Clinical Pathology
dc.contributor.otherNational Cancer Institute
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherModern Science and Arts University
dc.contributor.other6th October City
dc.contributor.otherGiza
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:42:08Z
dc.date.available2020-01-09T20:42:08Z
dc.date.issued2014
dc.descriptionScopus
dc.description.abstractThis study was designed to investigate the prevalence of metallo-?-lactamases (MBL) and extended-spectrum ?-lactamases (ESBL) in P. aeruginosa isolates collected from two different hospitals in Cairo, Egypt. Antibiotic susceptibility testing and phenotypic screening for ESBLs and MBLs were performed on 122 P. aeruginosa isolates collected in the period from January 2011 to March 2012. MICs were determined. ESBLs and MBLs genes were sought by PCR. The resistant rate to imipenem was 39.34%. The resistance rates for P. aeruginosa to cefuroxime, cefoperazone, ceftazidime, aztreonam, and piperacillin/tazobactam were 87.7%, 80.3%, 60.6%, 45.1%, and 25.4%, respectively. Out of 122 P. aeruginosa, 27% and 7.4% were MBL and ESBL, respectively. The prevalence of bla VIM2, bla OXA10-, bla VEB1, bla NDM-, and bla IMP1-like genes were found in 58.3%, 41.7%, 10.4%, 4.2%, and 2.1%, respectively. GIM-, SPM-, SIM-, and OXA-2-like genes were not detected in this study. OXA-10-like gene was concomitant with VIM-2 and/or VEB. Twelve isolates harbored both OXA-10 and VIM-2; two isolates carried both OXA-10 and VEB. Only one strain contained OXA-10, VIM-2, and VEB. In conclusion, bla VIM2- and bla OXA10-like genes were the most prevalent genes in P. aeruginosa in Egypt. To our knowledge, this is the first report of bla VIM2, bla IMP1, bla NDM, and bla OXA10 in P. aeruginosa in Egypt. � 2014 Mai M. Zafer et al.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=21100230018&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1155/2014/101635
dc.identifier.doiPubMed ID 24707471
dc.identifier.issn23146133
dc.identifier.otherhttps://doi.org/10.1155/2014/101635
dc.identifier.otherPubMed ID 24707471
dc.identifier.urihttps://t.ly/YZXev
dc.language.isoEnglishen_US
dc.relation.ispartofseriesBioMed Research International
dc.relation.ispartofseries2014
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectUniversity for Modern Sciences and Arts
dc.subjectMSA University
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectaztreonamen_US
dc.subjectcefoperazoneen_US
dc.subjectceftazidimeen_US
dc.subjectcefuroximeen_US
dc.subjectextended spectrum beta lactamaseen_US
dc.subjectimipenemen_US
dc.subjectmetallo beta lactamaseen_US
dc.subjectpiperacillin plus tazobactamen_US
dc.subjectbacterial DNAen_US
dc.subjectbeta lactamaseen_US
dc.subjectantibiotic resistanceen_US
dc.subjectarticleen_US
dc.subjectbacterial geneen_US
dc.subjectbacterial strainen_US
dc.subjectbacterium isolateen_US
dc.subjectbacterium isolationen_US
dc.subjectcancer patienten_US
dc.subjectcontrolled studyen_US
dc.subjectgenetic codeen_US
dc.subjecthumanen_US
dc.subjectminimum inhibitory concentrationen_US
dc.subjectmulticenter studyen_US
dc.subjectnonhumanen_US
dc.subjectphenotypeen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectprevalenceen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectbacterial geneen_US
dc.subjectEgypten_US
dc.subjectgeneticsen_US
dc.subjectmicrobiologyen_US
dc.subjectmultidrug resistanceen_US
dc.subjectneoplasmen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectbeta-Lactamasesen_US
dc.subjectDNA, Bacterialen_US
dc.subjectDrug Resistance, Multiple, Bacterialen_US
dc.subjectEgypten_US
dc.subjectGenes, Bacterialen_US
dc.subjectHumansen_US
dc.subjectNeoplasmsen_US
dc.subjectPseudomonas aeruginosaen_US
dc.titleAntimicrobial resistance pattern and their beta-lactamase encoding genes among Pseudomonas aeruginosa strains isolated from cancer patientsen_US
dc.typeArticleen_US
dcterms.isReferencedByWirth, F.W., Picoli, S.U., Cantarelli, V.V., Gon�alves, A.L., Brust, F.R., Santos, L.M., Barreto, M.F., Metallo- ? -lactamase-Producing pseudomonas aeruginosa in two hospitals from Southern Brazil (2009) Brazilian Journal of Infectious Diseases, 13 (3), pp. 170-172. , 2-s2.0-77749301581 10.1590/S1413-86702009000300003; Lambert, M.-L., Suetens, C., Savey, A., Palomar, M., Hiesmayr, M., Morales, I., Agodi, A., Wolkewitz, M., Clinical outcomes of health-care-associated infections and antimicrobial resistance in patients admitted to European intensive-care units: A cohort study (2011) The Lancet Infectious Diseases, 11 (1), pp. 30-38. , 2-s2.0-78650232703 10.1016/S1473-3099(10)70258-9; Keen III E., F., Robinson, B.J., Hospenthal, D.R., Aldous, W.K., Wolf, S.E., Chung, K.K., Murray, C.K., Prevalence of multidrug-resistant organisms recovered at a military burn center (2010) Burns, 36 (6), pp. 819-825. , 2-s2.0-77955578548 10.1016/j.burns.2009.10.013; Mendiratta, D.K., Deotale, V., Narang, P., Mettallo-?-lactamase producing Pseudomonas aeruginosa in a hospital from a rural area (2005) Indian Journal of Medical Research, 121 (5), pp. 701-703; Zhao, W.H., Hu, Z.Q., ? -Lactamases identified in clinical isolates of pseudomonas aeruginosa (2010) Critical Reviews in Microbiology, 36 (3), pp. 245-258. , 10.3109/1040841X.2010.481763; Wang, J., Zhou, J.-Y., Qu, T.-T., Shen, P., Wei, Z.-Q., Yu, Y.-S., Li, L.-J., Molecular epidemiology and mechanisms of carbapenem resistance in Pseudomonas aeruginosa isolates from Chinese hospitals (2010) International Journal of Antimicrobial Agents, 35 (5), pp. 486-491. , 2-s2.0-77949658199 10.1016/j.ijantimicag.2009.12.014; Mirsalehian, A., Feizabadi, M., Nakhjavani, F.A., Jabalameli, F., Goli, H., Kalantari, N., Detection of VEB-1, OXA-10 and PER-1 genotypes in extended-spectrum ? -lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients (2010) Burns, 36 (1), pp. 70-74. , 2-s2.0-72049123989 10.1016/j.burns.2009.01.015; Bauer, A.W., Kirby, W.M., Sherris, J.C., Turck, M., Antibiotic susceptibility testing by a standardized single disk method (1966) American Journal of Clinical Pathology, 45 (4), pp. 493-496. , 2-s2.0-0013902668; (2010) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standard M7-A7, , Clinical And Laboratory Standards Institute 7th Wayne, Pa, USA CLSI; Glupczynski, Y., Bogaerts, P., Deplano, A., Berhin, C., Huang, T.-D., Van Eldere, J., Rodriguez-Villalobos, H., Detection and characterization of class A extended-spectrum- ? -lactamase-producing Pseudomonas aeruginosa isolates in Belgian hospitals (2010) Journal of Antimicrobial Chemotherapy, 65 (5), pp. 866-871. , 2-s2.0-77953698632 10.1093/jac/dkq048; Bashir, D., Thokar, M.A., Fomda, B.A., Bashir, G., Zahoor, D., Ahmad, S., Toboli, A.S., Detection of metallo- ? -lactamase (MBL) producing Pseudomonas aeruginosa at a tertiary care hospital in Kashmir (2011) African Journal of Microbiology Research, 5 (2), pp. 164-172. , 2-s2.0-79953057442; Lee, S., Park, Y.-J., Kim, M., Lee, H.K., Han, K., Kang, C.S., Kang, M.W., Prevalence of Ambler class A and D ?-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea (2005) Journal of Antimicrobial Chemotherapy, 56 (1), pp. 122-127. , DOI 10.1093/jac/dki160; Alipour, T., Sadeghifard, N., Amirmozafari, N., Ghafurian, S., Abdulamir, A.S., Mohebi, R., Abu Bakar, F., Raftari, M., Incidence of extended spectrum ? -lactamase producing Pseudomonas aeruginosa and frequency of OXA-2 and OXA-10 genes (2010) Australian Journal of Basic and Applied Sciences, 4 (8), pp. 3202-3207. , 2-s2.0-78650258128; Yan, J.-J., Hsueh, P.-R., Ko, W.-C., Luh, K.-T., Tsai, S.-H., Wu, H.-M., Wu, J.-J., Metallo-?-lactamases in clinical Pseudomonas isolates in Taiwan and identification of VIM-3, a novel variant of the VIM-2 enzyme (2001) Antimicrobial Agents and Chemotherapy, 45 (8), pp. 2224-2228. , DOI 10.1128/AAC.45.8.2224-2228.2001; Ellington, M.J., Kistler, J., Livermore, D.M., Woodford, N., Multiplex PCR for rapid detection of genes encoding acquired metallo-?-lactamases [1] (2007) Journal of Antimicrobial Chemotherapy, 59 (2), pp. 321-322. , DOI 10.1093/jac/dkl481; Kaase, M., Nordmann, P., Wichelhaus, T.A., Gatermann, S.G., Bonnin, R.A., Poirel, L., NDM-2 carbapenemase in Acinetobacter baumannii from Egypt (2011) Journal of Antimicrobial Chemotherapy, 66 (6), pp. 1260-1262. , 2-s2.0-79955987012 10.1093/jac/dkr135; Mahmoud, B.A., Zahran, A.W., Hindawi, R.G., Labib, Z.A., Galal, R., Prevalence of multidrug-resistant Pseudomonas aeruginosa in patients with nosocomial infections at a university hospital in Egypt, with special reference to typing methods (2013) Journal of Virology & Microbiology, 2013, p. 13. , 290047 10.5171/2013.290047; Pic�o, R.C., Poirel, L., Gales, A.C., Nordmann, P., Diversity of ? -lactamases produced by ceftazidime-resistant Pseudomonas aeruginosa isolates causing bloodstream infections in Brazil (2009) Antimicrobial Agents and Chemotherapy, 53 (9), pp. 3908-3913. , 2-s2.0-70349148226 10.1128/AAC.00453-09; Ashour, H.M., El-Sharif, A., Species distribution and antimicrobial susceptibility of gram-negative aerobic bacteria in hospitalized cancer patients (2009) Journal of Translational Medicine, 714, pp. 1-13. , 2-s2.0-62849089702 10.1186/1479-5876-7-14; Mohamed, A.A., Shibl, A.M., Zaki, S.A., Tawfik, A.F., Antimicrobial resistance pattern and prevalence of metallo- ? -lactamases in Pseudomonas aeruginosa from Saudi Arabia (2011) African Journal of Microbiology Research, 5 (30), pp. 5528-5533. , 10.5897/AJMR11.1024; Souli, M., Galani, I., Giamarellou, H., Emergence of extensively drug-resistant and pandrug-resistant gram-negative bacilli in Europe (2008) Euro Surveillance, 13 (47), pp. 1-11. , 2-s2.0-58949091766; Bahar, M.A., Jamali, S., Samadikuchaksaraei, A., Imipenem-resistant Pseudomonas aeruginosa strains carry metallo- ? -lactamase gene bla VIM in a level i Iranian burn hospital (2010) Burns, 36 (6), pp. 826-830. , 2-s2.0-77955578394 10.1016/j.burns.2009.10.011; Mansour, S.A., Eldaly, O., Fatani, A.J., Mohamed, M.L., Ibrahim, E.M., Epidemiological characterization of P. Aeruginosa isolates of intensive care units in Egypt and Saudi Arabia (2013) Eastern Mediterranean Health Journal, 19 (1), pp. 71-80; De, A.S., Kumar, S.H., Baveja, S.M., Prevalence of metallo- ? -lactamase producing Pseudomonas aeruginosa and Acinetobacter species in intensive care areas in a tertiary care hospital (2010) Indian Journal of Critical Care Medicine, 14 (4), pp. 217-219. , 2-s2.0-79551640721 10.4103/0972-5229.76089; Walsh, T.R., Li, H., Toleman, M.A., Bennett, P.M., Jones, R.N., Complete sequence of p07-406, a 24,179-base-pair plasmid harboring the b l a VIM-7 metallo- ? -lactamase gene in a Pseudomonas aeruginosa isolate from the United States (2008) Antimicrobial Agents and Chemotherapy, 52 (9), pp. 3099-3105. , 2-s2.0-50949131890 10.1128/AAC.01093-07; Elias, J., Schoen, C., Heinze, G., Valenza, G., Gerharz, E., Riedmiller, H., Vogel, U., Nosocomial outbreak of VIM-2 metallo- ? -lactamase-producing Pseudomonas aeruginosa associated with retrograde urography (2010) Clinical Microbiology and Infection, 16 (9), pp. 1494-1500. , 2-s2.0-77956125376 10.1111/j.1469-0691.2010.03146.x; Hocquet, D., Pl�siat, P., Dehecq, B., Mariotte, P., Talon, D., Bertrand, X., Nationwide investigation of extended-spectrum ? -lactamases, metallo- ? -lactamases, and extended-spectrum oxacillinases produced by ceftazidime-resistant Pseudomonas aeruginosa strains in France (2010) Antimicrobial Agents and Chemotherapy, 54 (8), pp. 3512-3515. , 2-s2.0-77955365317 10.1128/AAC.01646-09; Poirel, L., Naas, T., Nordmann, P., Diversity, epidemiology, and genetics of class D ? -lactamases (2010) Antimicrobial Agents and Chemotherapy, 54 (1), pp. 24-38. , 2-s2.0-73849084148 10.1128/AAC.01512-08; Gharib, M.A., Ouda, N.H., Saeed, M., Hamed, L., Abdel Rahman, S.M., Prevalence of extended spectrum beta lactamase producers in an Egyptian Critical Care Center (2009) Egyptian Journal of Medical Microbiology, 18 (4), pp. 139-145; Jabalameli, F., Mirsalehian, A., Sotoudeh, N., Jabalameli, L., Aligholi, M., Khoramian, B., Taherikalani, M., Emaneini, M., Multiple-locus variable number of tandem repeats (VNTR) fingerprinting (MLVF) and antibacterial resistance profiles of extended spectrum beta lactamase (ESBL) producing Pseudomonas aeruginosa among burnt patients in Tehran (2011) Burns, 37 (7), pp. 1202-1207. , 2-s2.0-80052963168 10.1016/j.burns.2011.05.012; Shahcheraghi, F., Nikbin, V.-S., Feizabadi, M.M., Prevalence of ESBLs genes among multidrug-resistant isolates of Pseudomonas aeruginosa isolated from patients in Tehran (2009) Microbial Drug Resistance, 15 (1), pp. 37-39. , 2-s2.0-66749157997 10.1089/mdr.2009.0880; Wolter, D.J., Khalaf, N., Robledo, I.E., V�zquez, J.G., Sant�, I.M., Aquino, E.E., Goering, R.V., Hanson, N.D., Surveillance of carbapenem-resistant Pseudomonas aeruginosa isolates from Puerto Rican Medical Center Hospitals: Dissemination of KPC and IMP-18 ? -lactamases (2009) Antimicrobial Agents and Chemotherapy, 53 (4), pp. 1660-1664. , 2-s2.0-65649116984 10.1128/AAC.01172-08; Tawfik, A.F., Shibl, A.M., Aljohi, M.A., Altammami, M.A., Al-Agamy, M.H., Distribution of Ambler class A, B and D ? -lactamases among Pseudomonas aeruginosa isolates (2012) Burns, 38 (6), pp. 855-860. , 2-s2.0-84856904442 10.1016/j.burns.2012.01.005
dcterms.sourceScopus

Files

Original bundle

Now showing 1 - 2 of 2
Thumbnail Image
Name:
avatar_scholar_256.png
Size:
6.31 KB
Format:
Portable Network Graphics
Description:
Download
Thumbnail Image
Name:
101635.pdf
Size:
1.13 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Faculty Of Pharmacy Research Paper