C(-260)T polymorphism in CD14 receptor gene of Egyptians with acute myocardial infarction

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorHashad I.M.
dc.contributor.authorHossni N.M.
dc.contributor.authorAbdel Rahman M.F.
dc.contributor.authorShehata M.
dc.contributor.authorShaban G.
dc.contributor.authorGad M.Z.
dc.contributor.otherClinical Biochemistry Unit
dc.contributor.otherFaculty of Pharmacy and Biotechnology
dc.contributor.otherThe German University in Cairo
dc.contributor.otherCairo
dc.contributor.otherEgypt; Biochemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA)
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Cardiology
dc.contributor.otherFaculty of Medicine
dc.contributor.otherAin Shams University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Cardiology
dc.contributor.otherNational Heart Institute
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:09Z
dc.date.available2020-01-09T20:41:09Z
dc.date.issued2018
dc.descriptionScopus
dc.description.abstractBackground: Despite the significance of the traditional risk factors, recently published studies have suggested that inflammatory processes and variations in the genetics of the inflammatory system may participate in the initiation of atherosclerosis and its complications. Objective: To investigate the possible association between CD14 C(-260)T (rs2569190) gene polymorphism and the risk of acute myocardial infarction in the Egyptian population. Methods: We enrolled 100 acute myocardial infarction patients in addition to 107 healthy controls. Deoxyribonucleic acid was extracted, purified and used for the genotype assay of C(-260)T polymorphism in promoter region of CD14 gene. Genotyping was conducted using polymerase chain reaction-restriction fragment length polymorphism. Polymerase chain reaction product was digested using a restriction enzyme and the digestion products were specified. Serum CD14 levels were determined by Enzyme Linked Immunosorbent Assay. Results: CD14 genotypic distribution (CC: 15.9% vs. 16%, CT: 62.6% vs. 58%, TT: 21.5% vs. 26% in controls versus acute myocardial infarction patients, p > 0.05 for all variables) and allele frequencies (C allele: 47% vs., 45%, T allele: 52% vs. 55% in controls versus acute myocardial infarction patients, p > 0.05 for all variables) did not show a statistical significant difference. Serum CD14 levels were elevated in acute myocardial infarction patients (5.73�0.62 vs. 4.48�0.28 pg/ml, p < 0.05). However, there was no statistically significant difference in serum CD14 levels among different CD14 genotypes. Conclusion: CD14 C-(260)T polymorphism is not associated with incidence of acute myocardial infarction in Egyptians who showed elevated serum CD14 levels in comparison to healthy individuals. � 2018 Bentham Science Publishers.en_US
dc.identifier.doihttps://doi.org/10.2174/1389201019666180605125803
dc.identifier.doiPubMed ID 29874995
dc.identifier.issn13892010
dc.identifier.otherhttps://doi.org/10.2174/1389201019666180605125803
dc.identifier.otherPubMed ID 29874995
dc.identifier.urihttps://t.ly/KXX8p
dc.language.isoEnglishen_US
dc.publisherBentham Science Publishers B.V.en_US
dc.relation.ispartofseriesCurrent Pharmaceutical Biotechnology
dc.relation.ispartofseries19
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectUniversity for Modern Sciences and Arts
dc.subjectMSA University
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectAtherosclerosisen_US
dc.subjectCoronary artery diseaseen_US
dc.subjectGenotypesen_US
dc.subjectInflammationen_US
dc.subjectMyocardial infarctionen_US
dc.subjectPolymorphismen_US
dc.subjectCD14 antigenen_US
dc.subjectcholesterolen_US
dc.subjecthigh density lipoprotein cholesterolen_US
dc.subjectlow density lipoprotein cholesterolen_US
dc.subjectnitric oxideen_US
dc.subjectrestriction endonucleaseen_US
dc.subjecttriacylglycerolen_US
dc.subjectDNAen_US
dc.subjectlipopolysaccharide receptoren_US
dc.subjectacute heart infarctionen_US
dc.subjectadulten_US
dc.subjectArticleen_US
dc.subjectatherosclerosisen_US
dc.subjectcardiovascular risken_US
dc.subjectCD14 receptor geneen_US
dc.subjectcontrolled studyen_US
dc.subjectcoronary artery diseaseen_US
dc.subjectDNA extractionen_US
dc.subjectDNA polymorphismen_US
dc.subjectDNA purificationen_US
dc.subjectEgyptianen_US
dc.subjectenzyme linked immunosorbent assayen_US
dc.subjectfemaleen_US
dc.subjectgene expressionen_US
dc.subjectgene frequencyen_US
dc.subjectgene mutationen_US
dc.subjectgenetic associationen_US
dc.subjectgenetic variabilityen_US
dc.subjectgenotypeen_US
dc.subjecthumanen_US
dc.subjectincidenceen_US
dc.subjectinflammationen_US
dc.subjectmajor clinical studyen_US
dc.subjectmaleen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectpromoter regionen_US
dc.subjectprotein blood levelen_US
dc.subjectprotein expressionen_US
dc.subjectreceptor geneen_US
dc.subjectrestriction fragment length polymorphismen_US
dc.subjectacute diseaseen_US
dc.subjectatherosclerosisen_US
dc.subjectblooden_US
dc.subjectEgypten_US
dc.subjectgenetic polymorphismen_US
dc.subjectgenetic variationen_US
dc.subjectgeneticsen_US
dc.subjectheart infarctionen_US
dc.subjectmiddle ageden_US
dc.subjectsingle nucleotide polymorphismen_US
dc.subjectyoung adulten_US
dc.subjectAcute Diseaseen_US
dc.subjectAdulten_US
dc.subjectAtherosclerosisen_US
dc.subjectDNAen_US
dc.subjectEgypten_US
dc.subjectFemaleen_US
dc.subjectGene Frequencyen_US
dc.subjectGenetic Variationen_US
dc.subjectGenotypeen_US
dc.subjectHumansen_US
dc.subjectLipopolysaccharide Receptorsen_US
dc.subjectMaleen_US
dc.subjectMiddle Ageden_US
dc.subjectMyocardial Infarctionen_US
dc.subjectPolymorphism, Geneticen_US
dc.subjectPolymorphism, Single Nucleotideen_US
dc.subjectPromoter Regions, Geneticen_US
dc.subjectYoung Adulten_US
dc.titleC(-260)T polymorphism in CD14 receptor gene of Egyptians with acute myocardial infarctionen_US
dc.typeArticleen_US
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