The role of microRNA-31 and microRNA-21 as regulatory biomarkers in the activation of T lymphocytes of Egyptian lupus patients

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorAmr K.S.
dc.contributor.authorBayoumi F.S.
dc.contributor.authorElgengehy F.T.
dc.contributor.authorAbdallah S.O.
dc.contributor.authorAhmed H.H.
dc.contributor.authorEissa E.
dc.contributor.otherHead of Medical Molecular Genetics Department
dc.contributor.otherNational Research Centre
dc.contributor.otherCairo
dc.contributor.otherEgypt; Immunogenetics Department
dc.contributor.otherNational Research Centre
dc.contributor.otherCairo
dc.contributor.otherEgypt; Head of Microbiology and Immunology Department
dc.contributor.otherMSA University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Rheumatology and Rehabilitation
dc.contributor.otherFaculty of Medicine
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Faculty of Science
dc.contributor.otherCairo University
dc.contributor.otherGiza
dc.contributor.otherEgypt; Head of Medical Molecular Genetics Department
dc.contributor.otherNational Research Centre
dc.contributor.otherEl Buhouth St.
dc.contributor.otherCairo
dc.contributor.otherDokki 12622
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:33Z
dc.date.available2020-01-09T20:41:33Z
dc.date.issued2016
dc.descriptionScopus
dc.description.abstractSystemic lupus erythematosus (SLE) is an autoimmune disease characterized by familial aggregation and genetic predisposition. MicroRNAs (MiRNAs) serve as critical biomarkers in lupus patients because of their aberrant expression in different SLE stages. The study aimed to investigate the correlation of miR-31 and miR-21 with IL-2 in SLE patients as regulatory biomarkers in the activation of T lymphocytes of Egyptian lupus patients. Quantitative RT-PCR is carried out to estimate the expressions of miR-31 and miR-21, and IL-2 levels were determined using ELISA in plasma of 40 patients with SLE, 20 of their first-degree relatives and 20 healthy controls. The study also determined the systemic lupus erythematosus disease activity index (SLEDAI) score and proteinuria in SLE patients. The results revealed that miR-31 was lower expressed, while miR-21 was high expressed in SLE patients compared to their first-degree relatives and controls. MiR-31 was negatively correlated with SLEDAI and proteinuria in lupus patients, while miR-21 showed positive correlation with them. Also we found that there is a significant positive correlation between miR-31 and IL-2 in SLE patients, while miR-21 was negatively correlated with IL-2 level in patients. In conclusion, the study disclosed a significant association between miR-31 and miR-21 expression with IL-2 level in SLE patients. The regulatory biomarkers of miR-31 and miR-21 might have an impact on regulating IL-2 pathway expression and in turn on the activation of T lymphocytes in SLE. � 2016, Springer-Verlag Berlin Heidelberg.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=19773&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1007/s00296-016-3550-z
dc.identifier.doiPubMed ID 27510529
dc.identifier.issn1728172
dc.identifier.otherhttps://doi.org/10.1007/s00296-016-3550-z
dc.identifier.otherPubMed ID 27510529
dc.identifier.urihttps://t.ly/ZE6V9
dc.language.isoEnglishen_US
dc.publisherSpringer Verlagen_US
dc.relation.ispartofseriesRheumatology International
dc.relation.ispartofseries36
dc.subjectIL-2en_US
dc.subjectMiR-21en_US
dc.subjectMiR-31en_US
dc.subjectSLEen_US
dc.subjectbiological markeren_US
dc.subjectinterleukin 2en_US
dc.subjectmicroRNA 21en_US
dc.subjectmicroRNA 31en_US
dc.subjectbiological markeren_US
dc.subjectinterleukin 2en_US
dc.subjectmicroRNAen_US
dc.subjectMIRN21 microRNA, humanen_US
dc.subjectMIRN31 microRNA, humanen_US
dc.subjectadulten_US
dc.subjectArticleen_US
dc.subjectclinical articleen_US
dc.subjectcontrolled studyen_US
dc.subjectcorrelation analysisen_US
dc.subjectEgyptianen_US
dc.subjectenzyme linked immunosorbent assayen_US
dc.subjectfemaleen_US
dc.subjectfirst-degree relativeen_US
dc.subjectgene expressionen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectmaleen_US
dc.subjectpriority journalen_US
dc.subjectproteinuriaen_US
dc.subjectquantitative analysisen_US
dc.subjectreal time polymerase chain reactionen_US
dc.subjectSLEDAIen_US
dc.subjectsystemic lupus erythematosusen_US
dc.subjectT lymphocyte activationen_US
dc.subjectadolescenten_US
dc.subjectblooden_US
dc.subjectEgypten_US
dc.subjectgenetic association studyen_US
dc.subjectgeneticsen_US
dc.subjectimmunologyen_US
dc.subjectmetabolismen_US
dc.subjectmiddle ageden_US
dc.subjectseverity of illness indexen_US
dc.subjectsystemic lupus erythematosusen_US
dc.subjectT lymphocyteen_US
dc.subjectyoung adulten_US
dc.subjectAdolescenten_US
dc.subjectAdulten_US
dc.subjectBiomarkersen_US
dc.subjectEgypten_US
dc.subjectFemaleen_US
dc.subjectGenetic Association Studiesen_US
dc.subjectHumansen_US
dc.subjectInterleukin-2en_US
dc.subjectLupus Erythematosus, Systemicen_US
dc.subjectMaleen_US
dc.subjectMicroRNAsen_US
dc.subjectMiddle Ageden_US
dc.subjectSeverity of Illness Indexen_US
dc.subjectT-Lymphocytesen_US
dc.subjectYoung Adulten_US
dc.titleThe role of microRNA-31 and microRNA-21 as regulatory biomarkers in the activation of T lymphocytes of Egyptian lupus patientsen_US
dc.typeArticleen_US
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