IL-8 mRNA and serum levels in vitiligo patients: A case control study

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorFarag A.G.
dc.contributor.authorMarae A.H.
dc.contributor.authorHabib M.S.E.
dc.contributor.authorMahfouz R.Z.
dc.contributor.authorElnaidany N.F.
dc.contributor.authorYoussef S.I.
dc.contributor.authorEl-Feky S.M.
dc.contributor.otherDepartment of Dermatology
dc.contributor.otherAndrology and STDs
dc.contributor.otherFaculty of Medicine
dc.contributor.otherMenoufia University
dc.contributor.otherAl Minufiyah
dc.contributor.otherEgypt; Department of Medical Biochemistry
dc.contributor.otherFaculty of Medicine
dc.contributor.otherMenoufia University
dc.contributor.otherAl Minufiyah
dc.contributor.otherEgypt; Department of Clinical Pathology
dc.contributor.otherMenoufia University
dc.contributor.otherShebin Elkom
dc.contributor.otherEgypt; Cleveland Clinic
dc.contributor.otherLerner College of Medicine (CCLCM)
dc.contributor.otherCWR University
dc.contributor.otherCleveland
dc.contributor.otherOH
dc.contributor.otherUnited States; Department of Clinical Pharmacy
dc.contributor.otherFaculty of Pharmacy-MSA University
dc.contributor.otherOctober City
dc.contributor.otherEgypt; Department of Medical Microbiology and Immunology
dc.contributor.otherOctober 6 University
dc.contributor.otherEgypt; Department of Dermatology
dc.contributor.otherMinistry of Health
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:14Z
dc.date.available2020-01-09T20:41:14Z
dc.date.issued2017
dc.descriptionScopus
dc.descriptionMSA Google Scholar
dc.description.abstractIntroduction: Many theories are suggested to explain aetiology of vitiligo, such as autoimmune, genetic and biochemical. Interleukin-8 (IL-8) is a pro-inflammatory chemokine which is evaluated in the pathogenesis of some skin diseases, like psoriasis, however, in vitiligo, few studies were reported regarding this issue. Aim: The aim of the present study was to investigate serum level of IL-8 and IL-8 mRNA in patients with different types of vitiligo to validate its role in aetiopathogenesis of vitiligo. Materials and Methods: This case-control study was conducted on 39 vitiligo patients and 15 age and gender matched healthy controls. The participants were selected from Dermatology Outpatient Clinic, Menoufia University Hospitals from October, 2014 to October, 2015. They were subjected to clinical history, examination and full general examination. Detailed dermatological examination including VASI score was applied. IL-8 serum level was measured by ELISA and IL-8 mRNA was quantitated by Real Time -PCR. The statistical analysis was done using SPSS, version 20.0. Mann Whitney U test was used to compare two quantitative not normally distributed. Chi-square test (?2) was used to study association between two qualitative variables. Spearman correlation test was applied to assess correlation between two continuous quantitative variables. A p?0.05 was considered significant. Results: IL-8 serum level and mRNA concentration mean values were significantly elevated in vitiligo patients (26.25�43.28 pg/ mL and 8.48�11.92 ng/mL) than controls (0.57�0.50 pg/mL and 0.60�0.32 ng/mL) (p=0.002, for both), and they showed significant positive correlation with each other (r=0.622; p?0.001). Moreover, both of them revealed significant high values in localized vitiligo (62.20�74.39 pg/mL and 12.42�13.85 ng/mL) than generalized (18.39�29.49 pg/mL and 7.61�11.52 ng/mL) (p?0.000 and p=0.004), respectively. Conclusion: Serum IL-8 chemokine and its mRNA increased significantly in vitiligo patients indicating that it may have a dynamic role in vitiligo development and participate in its pathogenesis. � 2017, Journal of Clinical and Diagnostic Research. All rights reserved.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=16400154778&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.7860/JCDR/2017/30392.10857
dc.identifier.doiPubMed ID :
dc.identifier.issn2249782X
dc.identifier.otherhttps://doi.org/10.7860/JCDR/2017/30392.10857
dc.identifier.otherPubMed ID :
dc.identifier.urihttps://t.ly/m808A
dc.language.isoEnglishen_US
dc.publisherOxford University Press
dc.publisherJournal of Clinical and Diagnostic Researchen_US
dc.relation.ispartofseriesJournal of Clinical and Diagnostic Research
dc.relation.ispartofseries11
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectUniversity of Modern Sciences and Arts
dc.subjectMSA University
dc.subjectELISAen_US
dc.subjectIL-8 mRNAen_US
dc.subjectPathogenesisen_US
dc.subjectReal time PCRen_US
dc.subjectinterleukin 8en_US
dc.subjectmessenger RNAen_US
dc.subjectadulten_US
dc.subjectArticleen_US
dc.subjectcase control studyen_US
dc.subjectclinical articleen_US
dc.subjectcontrolled studyen_US
dc.subjectenzyme linked immunosorbent assayen_US
dc.subjectfemaleen_US
dc.subjecthumanen_US
dc.subjectmaleen_US
dc.subjectoxidative stressen_US
dc.subjectprotein expressionen_US
dc.subjectreal time polymerase chain reactionen_US
dc.subjectreverse transcription polymerase chain reactionen_US
dc.subjectvitiligoen_US
dc.titleIL-8 mRNA and serum levels in vitiligo patients: A case control studyen_US
dc.typeArticleen_US
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