Metabolic profile and hepatoprotective activity of the anthocyanin-rich extract of Hibiscus sabdariffa calyces

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorEzzat, Shahira M
dc.contributor.authorM Salama, Maha
dc.contributor.authorH Seif el-Din, Sayed
dc.contributor.authorSaleh, Samira
dc.contributor.authorM El-Lakkany, Naglaa
dc.contributor.authorA Hammam, Olfat
dc.contributor.authorB Salem, Maha
dc.contributor.authorS Botros, Sanaa
dc.date.accessioned2019-11-02T08:36:17Z
dc.date.available2019-11-02T08:36:17Z
dc.date.issued2016
dc.descriptionMSA Google Scholar
dc.description.abstractContext: Hibiscus sabdariffa L. (Malvaceae) is a common traditional tea that has many biological activities. Objectives: To evaluate the hepatoprotective effect and study the metabolic profile of the anthocyaninrich extract of H. sabdariffa calyces (HSARE). Materials and methods: The hepatoprotective activity of HSARE was assessed (100 mg/kg/d for 4 weeks) by examining the hepatic, inflammatory, oxidative stress markers and performing a histopathological examination in rats with thioacetamide (TAA)-induced hepatotoxicity. HSARE was analyzed using ultra-performance liquid chromatography-quadrupole-time-of-flight-photodiode array-mass spectrometry (UPLCqTOF-PDA-MS). Results: The UPLC-qTOF-PDA-MS analysis of HSARE enabled the identification of 25 compounds represented by delphinidin and its derivatives, cyanidin, kaempferol, quercetin, myricetin aglycones and glycosides, together with hibiscus lactone, hibiscus acid and caffeoylquinic acids. Compared to the TAAintoxicated group, HSARE significantly reduced the serum levels of alanine aminotransferase, aspartate aminotransferase and hepatic malondialdehyde by 37.96, 42.74 and 45.31%, respectively. It also decreased hepatic inflammatory markers, including tumour necrosis factor alpha, interleukin-6 and interferon gamma (INF-c), by 85.39, 14.96 and 70.87%, respectively. Moreover, it decreased the immunopositivity of nuclear factor kappa-B and CYP2E1 in liver tissue, with an increase in the effector apoptotic marker (caspase-3 positive cells), restoration of the altered hepatic architecture and increases in the activities of superoxide dismutase (SOD) and glutathione by 150.08 and 89.23%, respectively. Discussion and conclusion: HSARE revealed pronounced antioxidant and anti-inflammatory potential where SOD and INF-c were significantly improved. HSARE possesses the added value of being more water-soluble and of natural origin with fewer side effects expected compared to silymarin.en_US
dc.description.sponsorshipTaylor & Francisen_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=21082&tip=sid&clean=0
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dc.identifier.doihttps://doi.org/10.1080/13880209.2016.1214739
dc.identifier.issn1388-0209 (Print)
dc.identifier.issn1744-5116 (Online)
dc.identifier.otherhttps://doi.org/10.1080/13880209.2016.1214739
dc.identifier.urihttps://cutt.ly/MtwjqXm
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.relation.ispartofseriesPharmaceutical biology;54:12, 3172-3181
dc.subjectUltra-performance liquiden_US
dc.subjectchromatography-quadrupole-time-of-flight-PDAmass spectrometryen_US
dc.subjectdelphinidinen_US
dc.subjectHibiscus sabdariffa L.en_US
dc.subjectMalvaceaeen_US
dc.titleMetabolic profile and hepatoprotective activity of the anthocyanin-rich extract of Hibiscus sabdariffa calycesen_US
dc.typeArticleen_US

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