Comparative lyophilized platelet-rich plasma wafer and powder for wound-healing enhancement: formulation, in vitro and in vivo studies

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorYassin G.E.
dc.contributor.authorDawoud M.H.S.
dc.contributor.authorWasfi R.
dc.contributor.authorMaher A.
dc.contributor.authorFayez A.M.
dc.contributor.otherDepartment of Pharmaceutics and Industrial Pharmacy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAl Azhar University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Pharmaceutics
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA University)
dc.contributor.otherGiza
dc.contributor.otherEgypt; Department of Microbiology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA University)
dc.contributor.otherGiza
dc.contributor.otherEgypt; Department of Biochemistry
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA University)
dc.contributor.otherGiza
dc.contributor.otherEgypt; Department of Pharmacology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA University)
dc.contributor.otherGiza
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:35Z
dc.date.available2020-01-09T20:40:35Z
dc.date.issued2019
dc.descriptionScopus
dc.description.abstractPlatelet-rich plasma (PRP) accelerates wound healing, as it is an excellent source of growth factors. PRP was separated from whole human blood by centrifugation. PRP powder and wafers were prepared by lyophilization, with the wafers prepared using sodium carboxymethylcellulose (Na CMC). The PRP wafers showed porous structures, as indicated by scanning electron microscopy (SEM) images, and the ability of the wafer to absorb exudates and thus promote wound healing was tested with the hydration capacity test. The platelet count was tested and indicated that the presence of PRP in the wafers had no effect on the platelet count. An antimicrobial activity test was carried out, showing that PRP had antibacterial activity against Gram-negative bacteria. Compared with lyophilized PRP powder and PRP-free wafers, PRP wafers showed the highest percent of wound size reduction on induced wounds in rats. Histopathological examination of rat skin showed that the PRP wafers achieved the shortest healing time, followed by the lyophilized PRP powder and finally the PRP-free wafers. The present study revealed that PRP can be formulated as a wafer, which is a promising pharmaceutical delivery system that can be used for enhanced wound-healing activity and improved the ease of application compared to lyophilized PRP powder. 2019, 2019 Informa UK Limited, trading as Taylor & Francis Group.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=21190&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1080/03639045.2019.1620269
dc.identifier.doiPubMed ID 31099273
dc.identifier.issn3639045
dc.identifier.otherhttps://doi.org/10.1080/03639045.2019.1620269
dc.identifier.otherPubMed ID 31099273
dc.identifier.urihttps://t.ly/vj06Y
dc.language.isoEnglishen_US
dc.publisherTaylor and Francis Ltd.en_US
dc.relation.ispartofseriesDrug Development and Industrial Pharmacy
dc.relation.ispartofseries45
dc.subjecthistopathological studyen_US
dc.subjectlyophilization (freeze-drying)en_US
dc.subjectPlatelet-rich plasma (PRP)en_US
dc.subjectwaferen_US
dc.subjectwound healingen_US
dc.subjectcarboxymethylcelluloseen_US
dc.subjectwound healing promoting agenten_US
dc.subjectantiinfective agenten_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectanimal cellen_US
dc.subjectanimal experimenten_US
dc.subjectanimal tissueen_US
dc.subjectantibacterial activityen_US
dc.subjectArticleen_US
dc.subjectbacterial growthen_US
dc.subjectcontrolled studyen_US
dc.subjectdrug delivery systemen_US
dc.subjectepithelizationen_US
dc.subjectfreeze dryingen_US
dc.subjecthistopathologyen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjecthydrationen_US
dc.subjectin vitro studyen_US
dc.subjectin vivo studyen_US
dc.subjectmaleen_US
dc.subjectnonhumanen_US
dc.subjectphysical appearanceen_US
dc.subjectplatelet counten_US
dc.subjectpowderen_US
dc.subjectraten_US
dc.subjectscanning electron microscopyen_US
dc.subjectthrombocyte rich plasmaen_US
dc.subjectwaferen_US
dc.subjectwater lossen_US
dc.subjectwound closureen_US
dc.subjectwound healingen_US
dc.subjectzone of inhibitionen_US
dc.subjectadministration and dosageen_US
dc.subjectanimalen_US
dc.subjectchemistryen_US
dc.subjectdrug effecten_US
dc.subjectfreeze dryingen_US
dc.subjectGram negative bacteriumen_US
dc.subjectGram negative infectionen_US
dc.subjectmicrobiologyen_US
dc.subjectpowderen_US
dc.subjectproceduresen_US
dc.subjectskinen_US
dc.subjectthrombocyte rich plasmaen_US
dc.subjectWistar raten_US
dc.subjectwound healingen_US
dc.subjectAnimalsen_US
dc.subjectAnti-Bacterial Agentsen_US
dc.subjectCarboxymethylcellulose Sodiumen_US
dc.subjectFreeze Dryingen_US
dc.subjectGram-Negative Bacteriaen_US
dc.subjectGram-Negative Bacterial Infectionsen_US
dc.subjectMaleen_US
dc.subjectPlatelet-Rich Plasmaen_US
dc.subjectPowdersen_US
dc.subjectRatsen_US
dc.subjectRats, Wistaren_US
dc.subjectSkinen_US
dc.subjectWound Healingen_US
dc.titleComparative lyophilized platelet-rich plasma wafer and powder for wound-healing enhancement: formulation, in vitro and in vivo studiesen_US
dc.typeArticleen_US
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