BIOLOGICAL ACTIVITY AND STANDARDIZATION OF THE ETHANOLIC EXTRACT OF THE AERIAL PARTS OF MENTHA SUAVEOLENS EHRH
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Date
2014
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
World Journal of Pharmacy and Pharmaceutical Sciences
Series Info
World Journal of Pharmacy and Pharmaceutical Sciences;6
Doi
Scientific Journal Rankings
Abstract
A biologically guided fractionation of the ethanolic extract of the aerial
parts of Mentha suaveolens Ehrh. cultivated in Egypt revealed that the
ethyl acetate fraction had the highest hepatoprotective activity as it
prevented the increase caused by CCl4 in the levels of aspartate
aminotransferase (AST), alanine amino transferase (ALT) and alkaline
phosphatase (ALP) enzymes by 51.6%, 57.0% and 56.7%,
respectively. The same fraction also showed the highest antioxidant
activity; in vivo (as it restored the glutathione level in diabetic rats by
98.0 %) and in vitro as it had the highest free radical scavenging
activity (IC50=31g/ mL). It also had the highest cytotoxic activity against human liver
carcinoma cell line (HEPG2) (IC50 = 5.1 μg/ mL). Consequently, this fraction was purified to
yield caffeic acid and rosamarinic acid. Rosmarinic acid, the major component possessed
cytotoxic activity on HEPG2 (IC50 = 4.5 μg/ mL) comparable to doxorubicin (IC50 = 3.73
μg/mL). A rapid reversed-phase high-performance liquid chromatography (RP-HPLC)
method was developed for the standardization of the active ethyl acetate fraction and the
method was validated. Rosmarinic acid content in M. suaveolens was estimated by RPHPLC,
to be 1.16 and 50.17% w/w for the powdered aerial parts and ethyl acetate fraction,
respectively.
Description
Keywords
University of Cytotoxicity, Mentha Suaveolens
Citation
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