High performance liquid chromatography, TLC-densitometry, and first-derivative spectrophotometry for simultaneous determination of amlodipine and perindopril in bulk powder and its tablets
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Date
2013
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Series Info
Journal of Liquid Chromatography and Related Technologies
36
36
Scientific Journal Rankings
Abstract
Three simple, sensitive, and specific methods were developed for simultaneous determination of amlodipine besylate (AML) and Perindopril Erbumine (PER) without previous separation. The first method was dependent on the first derivative of the ratio spectra by measuring the amplitudes at 348 nm for amlodipine using 50 g mL-1 of perindopril as a divisor and at 227 nm for perindopril using 30 g mL-1 of amlodipine as a divisor. The second method was based on ion-pair RP-HPLC. Satisfactory resolution was achieved using RP-C18 chromatographic column Zorbax Extend column and a mobile phase consists of potassium dihydrogen phosphate buffer (0.05 M, pH 3.0 � 0.02 adjusted by orthophosphoric acid): acetonitrile 30:70 v/v at a flow rate 1 mL/min using 0.002 M sodium heptanesulfonate in the aqueous phase. UV detection was performed at 215 nm. The third method was based on TLC; the separation was carried out on Fluka TLC aluminum sheets silica gel 60 F254, using n-butanol:water:glacial acetic acid (4:5:1, v/v/v) as the mobile phase. The validation of the proposed methods was applied according to ICH guidelines and LOD and LOQ were calculated. The suggested methods were successfully applied for the determination of the cited drugs in bulk powder and commercial tablets. � 2013 Copyright Taylor and Francis Group, LLC.
Description
Scopus
Keywords
amlodipine besylate, COVERAM tablets, derivative ratio spectrophotometry, perindopril erbumine, RP-HPLC, TLS-Densitometry, Amlodipine besylate, COVERAM tablets, Derivative ratios, perindopril erbumine, RP-HPLC, TLS-Densitometry, High performance liquid chromatography, Separation, Silica gel, Spectrophotometry, Chromatography, acetic acid, acetonitrile, amlodipine besylate, butanol, perindopril tert butylamine, phosphoric acid, potassium dihydrogen phosphate, sodium heptanesulfonate, sulfonic acid derivative, unclassified drug, water, amplitude modulation, article, densitometry, drug determination, flow rate, high performance liquid chromatography, ion pair chromatography, powder, real time polymerase chain reaction, satisfaction, spectrophotometry, tablet formulation, ultraviolet spectrophotometry, validation process