Dual approach for the colorimetric determination of unamplified microRNAs by using citrate capped gold nanoparticles

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorNossier A.I.
dc.contributor.authorAbdelzaher H.
dc.contributor.authorMatboli M.
dc.contributor.authorEissa S.
dc.contributor.otherBiochemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherMisr University for Science and Technology (MUST)
dc.contributor.other6th October City
dc.contributor.otherGiza
dc.contributor.otherEgypt; Faculty of Biotechnology
dc.contributor.otherOctober University for Modern Sciences & Arts
dc.contributor.other6th October City
dc.contributor.otherCairo
dc.contributor.otherEgypt; Oncology Diagnostic Unit
dc.contributor.otherMedical Biochemistry & Molecular Biology Department
dc.contributor.otherFaculty of Medicine
dc.contributor.otherAin Shams University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Faculty of Medicine Ain Shams Research Institute (MASRI)
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:56Z
dc.date.available2020-01-09T20:40:56Z
dc.date.issued2018
dc.descriptionScopus
dc.description.abstractThe authors describe a method for the colorimetric determination of unamplified microRNA. It is based on the use of citrate-capped gold nanoparticles (AuNPs) and, alternatively, a microRNA-probe hybrid or a magnetically extracted microRNA that serve as stabilizers against the salt-induced aggregation of AuNPs. The absorbance ratios A525/A625 of the reacted AuNP solutions were used to quantify the amount of microRNA. The assay works in the range of 5�25�pmol microRNA. The lower limit of detection (LOD) is 10�pmol. The performance of the method was tested by detection of microRNA-210-3p in totally extracted urinary microRNA from normal, benign, and bladder cancer subjects. The sensitivity and specificity for qualitative detection of urinary microRNA-210-3p using the assay are 74% and 88% respectively, which is consistent with real time PCR based assays. The assay was applied to the determination of specific microRNA by using its specific oligo targeter or following magnetic isolation of the desired microRNA. The method is simple, cost-efficient, has a short turn-around time and requires minimal equipment and personnel. � 2018, Springer-Verlag GmbH Austria, part of Springer Nature.en_US
dc.identifier.doihttps://doi.org/10.1007/s00604-018-2767-9
dc.identifier.doiPubMedID29594755
dc.identifier.issn263672
dc.identifier.otherhttps://doi.org/10.1007/s00604-018-2767-9
dc.identifier.otherPubMedID29594755
dc.identifier.urihttps://t.ly/rxxeW
dc.language.isoEnglishen_US
dc.publisherSpringer-Verlag Wienen_US
dc.relation.ispartofseriesMicrochimica Acta
dc.relation.ispartofseries185
dc.subjectAuNPsen_US
dc.subjectBladder canceren_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectMicroRNA detectionen_US
dc.subjectMicroRNA-210-3pen_US
dc.subjectOligonucleotide adsorptionen_US
dc.subjectOligotargeteren_US
dc.subjectSalt-induced aggregationen_US
dc.subjectStreptavidinen_US
dc.subjectcitric aciden_US
dc.subjectgolden_US
dc.subjectmetal nanoparticleen_US
dc.subjectmicroRNAen_US
dc.subjectMIRN210 microRNA, humanen_US
dc.subjectchemistryen_US
dc.subjectcolorimetryen_US
dc.subjectcost benefit analysisen_US
dc.subjecteconomicsen_US
dc.subjecthumanen_US
dc.subjectisolation and purificationen_US
dc.subjectproceduresen_US
dc.subjecturineen_US
dc.subjectCitric Aciden_US
dc.subjectColorimetryen_US
dc.subjectCost-Benefit Analysisen_US
dc.subjectGolden_US
dc.subjectHumansen_US
dc.subjectMetal Nanoparticlesen_US
dc.subjectMicroRNAsen_US
dc.titleDual approach for the colorimetric determination of unamplified microRNAs by using citrate capped gold nanoparticlesen_US
dc.typeArticleen_US
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