Dapagliflozin modulates neuronal injury via instigation of LKB1/p-AMPK/GABAB R2 signaling pathway and suppression of the inflammatory cascade in an essential tremor rat model

Abstract

Background: Although, disturbances in cellular energy demarcate the neuronal hyperexcitability in essential tremor (ET), nevertheless, no available data relates energy sensors and GABAergic neurotransmission in ET. Noteworthy, reports have asserted dapagliflozin’s (DAPA) role in enhancing autophagic sensors in other disorders. Herein, this study aimed to investigate DAPA’s impact on the GABAB receptor subunit (GABAB R2), notwithstanding the GABA A involvement, in an ET model.

Methods: ET was induced by a single dose of harmaline (30 mg/kg; i.p.) while DAPA (1mg/kg/day; p.o.) was given for 5 days before ET induction. The autophagic sensors were examined by injecting a single dose of dorsomorphin (DORSO) AMPK inhibitor (0.2 mg/kg; i.p.) on the 5th day before ET induction.

Results: DAPA decreased HAR-induced tremor score and alleviated motor disabilities observed in the open field, rotarod, wire grip strength, and gait kinematics confirmed by reduced electrical activity in electroencephalogram. In the cerebella, DAPA curbed HAR-evoked inflammatory cytokines, apoptotic markers, and glutamate while restoring the disturbed GABA, BDNF, LKB1, p-AMPK, and GABAB R2 levels. DAPA’s effect was mostly obliterated by DORSO.

Conclusion: DAPA offers a potential neuroprotective effect in ET by augmenting the neuronal inhibitory machinery via suppressing the inflammatory and excitotoxicity systems thru LKB1/p-AMPK/GABAB R2 signaling.