Symphytum species: A comprehensive review on chemical composition, food applications and phytopharmacology

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorSalehi B.
dc.contributor.authorSharopov F.
dc.contributor.authorTumer T.B.
dc.contributor.authorOzleyen A.
dc.contributor.authorRodrguez-Prez C.
dc.contributor.authorEzzat S.M.
dc.contributor.authorAzzini E.
dc.contributor.authorHosseinabadi T.
dc.contributor.authorButnariu M.
dc.contributor.authorSarac I.
dc.contributor.authorBostan C.
dc.contributor.authorAcharya K.
dc.contributor.authorSen S.
dc.contributor.authorKasapoglu K.N.
dc.contributor.authorDa?kaya-Dikmen C.
dc.contributor.authorzelik B.
dc.contributor.authorBaghalpour N.
dc.contributor.authorSharifi-Rad J.
dc.contributor.authorFokou P.V.T.
dc.contributor.authorCho W.C.
dc.contributor.authorMartins N.
dc.contributor.otherSchool of Medicine
dc.contributor.otherBam University of Medical Sciences
dc.contributor.otherBam
dc.contributor.other44340847
dc.contributor.otherIran; Department of Pharmaceutical Technology
dc.contributor.otherAvicenna Tajik State Medical University
dc.contributor.otherRudaki 139
dc.contributor.otherDushanbe
dc.contributor.other734003
dc.contributor.otherTajikistan; Department of Molecular Biology and Genetics
dc.contributor.otherFaculty of Arts and Science
dc.contributor.otherCanakkale Onsekiz Mart University
dc.contributor.otherCanakkale
dc.contributor.other17020
dc.contributor.otherTurkey; Graduate Program of Biomolecular Sciences
dc.contributor.otherInstitute of Natural and Applied Sciences
dc.contributor.otherCanakkale Onsekiz Mart University
dc.contributor.otherCanakkale
dc.contributor.other17020
dc.contributor.otherTurkey; Department of Nutrition and Food Science
dc.contributor.otherUniversity of Granada
dc.contributor.otherCampus of Cartuja
dc.contributor.otherGranada
dc.contributor.otherE-18071
dc.contributor.otherSpain; Institute of Nutrition and Food Technology (INYTA) 'Jos Mataix'
dc.contributor.otherBiomedical Research Centre
dc.contributor.otherUniversity of Granada
dc.contributor.otherAvenida del Conocimiento s/n
dc.contributor.otherGranada
dc.contributor.otherE-18071
dc.contributor.otherSpain; Pharmacognosy Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherKasr El-Ainy Street
dc.contributor.otherCairo
dc.contributor.other11562
dc.contributor.otherEgypt; Department of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Science and Arts (MSA)
dc.contributor.other6th October
dc.contributor.other12566
dc.contributor.otherEgypt; Centre for Research on Food and Nutrition
dc.contributor.otherCouncil for Agricultural Research and Economics
dc.contributor.otherRome
dc.contributor.other546-00178
dc.contributor.otherItaly; Department of Pharmacognosy and Biotechnology
dc.contributor.otherSchool of Pharmacy
dc.contributor.otherShahid Beheshti University of Medical Sciences
dc.contributor.otherTehran
dc.contributor.other11369
dc.contributor.otherIran; Banat's University of Agricultural Sciences and Veterinary Medicine King Michael I of Romania from Timisoara
dc.contributor.otherCalea Aradului 119
dc.contributor.otherTimis
dc.contributor.other300645
dc.contributor.otherRomania; Molecular and Applied Mycology and Plant Pathology Laboratory
dc.contributor.otherDepartment of Botany
dc.contributor.otherUniversity of Calcutta
dc.contributor.otherKolkata
dc.contributor.other700019
dc.contributor.otherIndia; Department of Botany
dc.contributor.otherFakir Chand College
dc.contributor.otherDiamond Harbour
dc.contributor.otherWest Bengal 743331
dc.contributor.otherIndia; Istanbul Technical University
dc.contributor.otherChemical and Metallurgical Engineering Faculty
dc.contributor.otherFood Engineering Department
dc.contributor.otherAyaza ga Campus
dc.contributor.otherMaslak
dc.contributor.otherIstanbul
dc.contributor.other34469
dc.contributor.otherTurkey; Istanbul Gedik University
dc.contributor.otherDepartment of Gastronomy and Culinary Arts
dc.contributor.otherKartal
dc.contributor.otherIstanbul
dc.contributor.other34876
dc.contributor.otherTurkey; Bioactive Research and Innovation Food Manufac.
dc.contributor.otherIndust. Trade Ltd.
dc.contributor.otherKatar Street
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dc.contributor.otherB110
dc.contributor.otherSar?yer
dc.contributor.otherIstanbul
dc.contributor.other34467
dc.contributor.otherTurkey; Phytochemistry Research Center
dc.contributor.otherShahid Beheshti University of Medical Sciences
dc.contributor.otherTehran
dc.contributor.other11369
dc.contributor.otherIran; Zabol Medicinal Plants Research Center
dc.contributor.otherZabol University of Medical Sciences
dc.contributor.otherZabol
dc.contributor.other61615-585
dc.contributor.otherIran; Antimicrobial and Biocontrol Agents Unit
dc.contributor.otherDepartment of Biochemistry
dc.contributor.otherFaculty of Science
dc.contributor.otherUniversity of Yaounde 1
dc.contributor.otherNgoa Ekelle
dc.contributor.otherAnnex Fac. Sci
dc.contributor.otherYaounde
dc.contributor.other812
dc.contributor.otherCameroon; Department of Clinical Oncology
dc.contributor.otherQueen Elizabeth Hospital
dc.contributor.other30 Gascoigne Road
dc.contributor.otherHong Kong
dc.contributor.otherHong Kong; Faculty of Medicine
dc.contributor.otherUniversity of Porto
dc.contributor.otherAlameda Prof. Hernni Monteiro
dc.contributor.otherPorto
dc.contributor.other4200-319
dc.contributor.otherPortugal; Institute for Research and Innovation in Health (i3S)
dc.contributor.otherUniversity of Porto
dc.contributor.otherPorto
dc.contributor.other4200-135
dc.contributor.otherPortugal
dc.date.accessioned2020-01-09T20:40:47Z
dc.date.available2020-01-09T20:40:47Z
dc.date.issued2019
dc.descriptionScopus
dc.descriptionMSA Google Scholar
dc.description.abstractSymphytum species belongs to the Boraginaceae family and have been used for centuries for bone breakages, sprains and rheumatism, liver problems, gastritis, ulcers, skin problems, joint pain and contusions, wounds, gout, hematomas and thrombophlebitis. Considering the innumerable potentialities of the Symphytum species and their widespread use in the world, it is extremely important to provide data compiling the available literature to identify the areas of intense research and the main gaps in order to design future studies. The present review aims at summarizing the main data on the therapeutic indications of the Symphytum species based on the current evidence, also emphasizing data on both the efficacy and adverse effects. The present review was carried out by consulting PubMed (Medline), Web of Science, Embase, Scopus, Cochrane Database, Science Direct and Google Scholar (as a search engine) databases to retrieve the most updated articles on this topic. All articles were carefully analyzed by the authors to assess their strengths and weaknesses, and to select the most useful ones for the purpose of review, prioritizing articles published from 1956 to 2018. The pharmacological effects of the Symphytum species are attributed to several chemical compounds, among them allantoin, phenolic compounds, glycopeptides, polysaccharides and some toxic pyrrolizidine alkaloids. Not less important to highlight are the risks associated with its use. In fact, there is increasing consumption of over-the-counter drugs, which when associated with conventional drugs can cause serious and even fatal adverse events. Although clinical trials sustain the folk topical application of Symphytum species in musculoskeletal and blunt injuries, with minor adverse effects, its antimicrobial potency was still poorly investigated. Further studies are needed to assess the antimicrobial spectrum of Symphytum species and to characterize the active molecules both in vitro and in vivo. 2019 by the authors.en_US
dc.identifier.doihttps://doi.org/10.3390/molecules24122272
dc.identifier.doiPubMedID
dc.identifier.otherhttps://doi.org/10.3390/molecules24122272
dc.identifier.otherPubMedID
dc.identifier.urihttps://t.ly/Ggxyr
dc.relation.ispartofseriesMolecules
dc.relation.ispartofseries24
dc.subjectBiological activityen_US
dc.subjectClinical trialsen_US
dc.subjectPhytochemistryen_US
dc.subjectSymphytum speciesen_US
dc.subjectantiinfective agenten_US
dc.subjectantioxidanten_US
dc.subjectphytochemicalen_US
dc.subjectanimalen_US
dc.subjectBoraginaceaeen_US
dc.subjectchemistryen_US
dc.subjectdrug effecten_US
dc.subjectecosystemen_US
dc.subjectfood preservationen_US
dc.subjecthumanen_US
dc.subjectmusculoskeletal diseaseen_US
dc.subjectphysiologyen_US
dc.subjecttreatment outcomeen_US
dc.subjectwound healingen_US
dc.subjectAnimalsen_US
dc.subjectAnti-Infective Agentsen_US
dc.subjectAntioxidantsen_US
dc.subjectBoraginaceaeen_US
dc.subjectEcosystemen_US
dc.subjectFood Preservationen_US
dc.subjectHumansen_US
dc.subjectMusculoskeletal Diseasesen_US
dc.subjectPhytochemicalsen_US
dc.subjectTreatment Outcomeen_US
dc.subjectWound Healingen_US
dc.titleSymphytum species: A comprehensive review on chemical composition, food applications and phytopharmacologyen_US
dcterms.isReferencedBySchmelzer, G., Gurib-Fakim, A., (2008) Plant Resources of Tropical Africa: Medicinal Plants 1, , Backhuys Publishers CTA: Kerkwerve, The Netherlands; Horinouchi, C.D., Otuki, M.F., Botanical briefs: Comfrey (Symphytum officinale) (2013) Cutis, 91, pp. 225-228; Frost, R., MacPherson, H., O'Meara, S.M., A critical scoping review of external uses of comfrey (Symphytum spp.) (2013) Complementary Ther. Med., 21, pp. 724-745; Staiger, C., Comfrey: A clinical overview (2012) Phytother. Res., 26, pp. 1441-1448; Riet-Correa, F., Medeiros, R., Tokarnia, C., Dobereiner, J., Toxic plants for livestock in Brazil: Economic impact, toxic species, control measures and public health implications (2007) Poisonous Plants: Global Research and Solutions, pp. 2-14. , Panter, K.E., Wierenga, T.L., Pfister, J.A., Eds.; CABI Press: Wallingford, UK; Cameron, M., Chrubasik, S., Topical herbal therapies for treating osteoarthritis (2013) Cochrane Database Syst. Rev., p. Cd010538; MacKay, D., Miller, A.L., Nutritional support for wound healing (2003) Altern. Med. Rev., 8, pp. 359-377; Neagu, E., Paun, G., Radu, G., Antioxidant capacity of some Symphytum officinalis extracts processed by ultrafiltration (2010) Rom. Biotechnol. Lett., 15, pp. 5505-5511; Sowa, I., Paduch, R., Strzemski, M., Zielinska, S., Rydzik-Strzemska, E., Sawicki, J., Kocjan, R., Latalski, M., Proliferative and antioxidant activity of Symphytum officinale root extract (2018) Nat. Prod. Res., 32, pp. 605-609; Barbakadze, V., Mulkijanyan, K., Merlani, M.M., Gogilashvili, L., Amiranashvili, L., Shaburishvili, K., Extraction, composition and the antioxidant and anticomplement activities of high molecular weight fractions from the leaves of Symphytum asperum and S. Caucasicum (2011) Pharm. Chem. J., 44, pp. 604-607; Chen, S., Shang, H., Yang, J., Li, R., Wu, H., Effects of different extraction techniques on physicochemical properties and activities of polysaccharides from comfrey (Symphytum officinale L.) root (2018) Ind. Crop. Prod., 121, pp. 18-25; Brown, A.W., Stegelmeier, B.L., Colegate, S.M., Gardner, D.R., Panter, K.E., Knoppel, E.L., Hall, J.O., The comparative toxicity of a reduced, crude comfrey (Symphytum officinale) alkaloid extract and the pure, comfrey-derived pyrrolizidine alkaloids, lycopsamine and intermedine in chicks (Gallus gallus domesticus) (2016) J. Appl. Toxicol., 36, pp. 716-725; Kurucu, S., Kartal, M., Choudary, M.I., Topcu, G., Pyrrolizidine Alkaloids from Symphytum sylvaticum Boiss. SubSp. Sepulcrale. (Boiss. & Bal.) Greuter & Burdet var. Sepulcrale and Symphytum aintabicum Hub. - Mor. & Wickens (2002) Turk. J. Chem., 26, pp. 195-199; Mei, N., Guo, L., Fu, P.P., Fuscoe, J.C., Luan, Y., Chen, T., Metabolism, Genotoxicity, annd Carcinogenicity of Comfrey (2010) J. Toxicol. Environ. Health Part B, 13, pp. 509-526; Adeneye, A.A., 6 - Subchronic and chronic toxicities of African medicinal plants (2014) Toxicological Survey of African Medicinal Plants, , Kuete, Ed.; Elsevier: Amsterdam, The Netherlands, CrossRef; Kruse, L.H., Stegemann, T., Sievert, C., Ober, D., Identification of a second site of pyrrolizidine alkaloid biosynthesis in comfrey to boost plant defense in floral stage() (2017) Plant Physiol, 174, pp. 47-55; Mazzocchi, A., Montanaro, F., Observational study of the use of Symphytum 5CH in the management of pain and swelling after dental implant surgery (2012) Homeopathy, 101, pp. 211-216; Colegate, S.M., Stegelmeier, B.L., Edgar, J.A., 14 - Dietary exposure of livestock and humans to hepatotoxic natural products (2012) Animal Feed Contamination, , Fink-Gremmels, J., Ed.; Woodhead Publishing: Amsterdam, The Netherlands, CrossRef; Singh, H., Du, J., Singh, P., Yi, T.H., Role of green silver nanoparticles synthesized from Symphytum officinale leaf extract in protection against UVB-induced photoaging (2018) J. Nanostructure Chem., 8, pp. 359-368; Karavaev, V.A., Solntsev, M.K., Iurina, T.P., Iurina, E.V., Poliakova, I.B., Kuznetsov, A.M., Antifungal activity of aqueous extracts from the leaf of cowparsnip and comfrey (2001) Izv. Akad. Nauk. Seriia Biol., 4, pp. 435-441; Pileggi, M., Raiman, P.M., Micheli, A., Beatriz, S., Bobalto, V., Antimicrobial Action and Endophytic interaction in Symphytum officinale L (2002) Publ. Uepg, 8, pp. 47-55; Avancini, C., Wiest, J.M., Dall'Agnol, R., Haas, J.S., Poser, G.L., Antimicrobial activity of plants used in the prevention and control of bovine mastitis in Southern Brazil (2008) Lat. Am. J. Pharm., 27, pp. 894-899; Rodrigues Oliveira, P., Ramos Santos, F., Ferreira Duarte, E., Silva Guimar�es, G., Sartori Carvalho Mattos, N., Minafra, C., Symbiotics and Aloe vera and Symphytum officinale extracts in broiler feed (2016) Semin.: Cincias Agrrias, 37, p. 2677; Pawlowski, B., Symphytum L. And procopiania gusul (1972) Flora of Europea, 3, pp. 103-106. , Tutin, T., Heywood, Burges, H., Moore, N.A., Valentine, D.M., Walters, D.H., Webb, S.M., Eds.; Cambridge University Press: Cambridge, UK; Peruzzi, L., Garbari, F., Bottega, S., Symphytum tanaicense (Boraginaceae) new for the Italian flora (2001) Willdenowia, 31, pp. 33-41; Tar?kahya, B., (2010) The Revision of Turkish Symphytum L. (Boraginaceae) Genus, , Hacettepe University: Ankara, Turkey; Hac?oglu, B.T., Erik, S., Phylogeny of Symphytum L.(Boraginaceae) with special emphasis on Turkish species (2011) Afr. J. Biotechnol., 10, pp. 15483-15493; Teynor, T.M., Putnam, D.H., Doll, J.D., Kelling, K.A., Oelke, E.A., Undersander, D.J., Oplinger, E.S., (1992) Comfrey - Alternative Field Crops Manual, , Minnesota Extension Service, University of Minnesota: Minneapolis, MN, USA; Chittendon, F., (1956) RHS Dictionary of Plants plus Supplement, , Oxford University Press: Oxford, UK; Grieve, B., (1984) A Modern Herbal, , Penguin: London, UK; Huxley, A., (1992) The New RHS Dictionary of Gardening, , MacMillan Press: London, UK; Bogert, L.J., Briggs, G.M., Calloway, D.H., (1973) Nutrition and Physical Fitness, , W.B. Saunders Co.: Philadelphia, PA, USA; Harris, P.J.C., Grove, C.G., Havard, A.J., In vitro propagation of Symphytum species (1989) Sci. Hortic., 40, pp. 275-281; Robinson, R.G., (1983) Comfrey-A Controversial Crop, , University of Minnesota. Agricultural Experiment Station: St. Paul, MN, USA; Thoresen, E.M., (2013) Symphytum Officinale Common Comfrey, , Szent Istvan University: Budapest, Hungary; Bellardi, M.G., Benni, A., The occurrence of alfalfa mosaic virus in Symphytum tuberosum L (2005) J. Plant Pathol., 87, p. 75; Juhl, V.M., Liste over vaertplanter for blad nematoden Aphelenchoides ritzemabosi (1978) Ugeskr. Agron. HortonomerForstkandidater Og Licent, 123, pp. 183-186; Knight, K.W., Barber, C.J., Page, G.D., Plant-parasitic Nematodes of New Zealand Recorded by Host Association (1997) J. Nematol., 29, pp. 640-656; Neagu, E., P?un, G., Radu, L.G., Phytochemical study of some Symphytum officinalis extracts concentrated by membranous procedures (2011) Sci. Bull. -Univ. Politeh. Buchacharest, pp. 3-7; Stickel, F., Seitz, H.K., The efficacy and safety of comfrey (2000) Public Health Nutr, 3, pp. 501-508; Trifan, A., Opitz, S.E.W., Josuran, R., Grubelnik, A., Esslinger, N., Peter, S., Brm, S., Wolfram, E., Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots (2018) Food Chem. Toxicol., 112, pp. 178-187; Mulkijanyan, K., Barbakadze, V., Novikova, Z., Sulakvelidze, M., Gogilashvili, L., Amiranashvili, L., Merlani, M., Burn healing compositions from Caucasian species of comfrey (Symphytum L.) (2009) Bull. Georg. Natl. Acad. Sci., 3, pp. 114-117; Roeder, E., Bourauel, T., Neuberger, V., Symviridine, a new pyrrolizidine alkaloid from Symphytum species (1992) Phytochemistry, 31, pp. 4041-4042; Roeder, E., Medicinal plants in Europe containing pyrrolizidine alkaloids (1995) Pharmazie, 50, pp. 83-98; Onduso, S.O., (2014) Determination of Levels of Pyrrolizidine Alkaloids in Symphytum Asperum Lepech Growing in Selected Parts of Keniya, , Kenyatta University: Nairobi, Kenia; Barbakadze, V.V., Kemertelidze, E.P., Shashkov, A.S., Usov, A.I., Structure of a new anticomplementary dihydroxycinnamate-derived polymer from Symphytum asperum (Boraginaceae) (2000) Mendeleev Commun, 10, pp. 148-149; Barbakadze, V., Kemertelidze, E., Targamadze, I., Mulkijanyan, K., Shashkov, A.S., Usov, A.I., Poly[3-(3,4-dihydroxyphenyl)glyceric acid], a new biologically active polymer from Symphytum asperum Lepech. And S. Caucasicum Bieb. (Boraginaceae) (2005) Molecules, 10, pp. 1135-1144; Barbakadze, V.V., Kemertelidze, E.P., Mulkijanyan, K.G., Van Den Berg, A.J.J., Beukelman, C.J., Van Den Worm, E., Quarles Van Ufford, H.C., Usov, A.I., Antioxidant and anticomplement activity of poly[3-(3,4-dihydroxyphenyl) glyceric acid] from Symphytum asperum and Symphytum caucasicum plants (2007) Pharm. Chem. J., 41, pp. 14-16; Melkumova, Z.V., Telezhenetskaya, M.V., Yunusov, S.Y., Manko, I.V., Refinement of the structure of asperumine (1974) Chem. Nat. Compd., 10, pp. 483-485; Mroczek, T., Ndjoko-Ioset, K., G?owniak, K., Mietkiewicz-Capa?a, A., Hostettmann, K., Investigation of Symphytum cordatum alkaloids by liquid-liquid partitioning, thin-layer chromatography and liquid chromatography-ion-trap mass spectrometry (2006) Anal. Chim. Acta, 566, pp. 157-166; Avula, B., Sagi, S., Wang, Y.H., Zweigenbaum, J., Wang, M., Khan, I.A., Characterization and screening of pyrrolizidine alkaloids and N-oxides from botanicals and dietary supplements using UHPLC-high resolution mass spectrometry (2015) Food Chem, 178, pp. 136-148; Liu, F., Wan, S.Y., Jiang, Z., Li, S.F.Y., Ong, E.S., Osorio, J.C.C., Determination of pyrrolizidine alkaloids in comfrey by liquid chromatography-electrospray ionization mass spectrometry (2009) Talanta, 80, pp. 916-923; Kim, N.C., Oberlies, N.H., Brine, D.R., Handy, R.W., Wani, M.C., Wall, M.E., Isolation of symlandine from the roots of common comfrey (Symphytum officinale) using countercurrent chromatography (2001) J. Nat. Prod., 64, pp. 251-253; Jane, D., Kreft, S., TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound (2014) Acta. Pharm., 64, pp. 503-508; Janes, D., Kalamar, B., Kreft, S., Improved method for isolation of lycopsamine from roots of comfrey (Symphytum officinale) (2012) Nat. Prod. Commun., 7, pp. 861-862; Brauchli, J., Lthy, J., Zweifel, U., Schlatter, C., Pyrrolizidine alkaloids from Symphytum officinale L. And their percutaneous absorption in rats (1982) Experientia, 38, pp. 1085-1087; Altamirano, J.C., Gratz, S.R., Wolnik, K.A., Investigation of pyrrolizidine alkaloids and their N-oxides in commercial comfrey-containing products and botanical materials by liquid chromatography electrospray ionization mass spectrometry (2005) J. Aoac Int., 88, pp. 406-412; Tsutomu, F., Hikich, M., Alkaloids and triterpenoids of Symphytum officinale (1971) Phytochemistry, 10, pp. 2217-2220; Dresler, S., Szymczak, G., Wojcik, M., Comparison of some secondary metabolite content in the seventeen species of the Boraginaceae family (2017) Pharm. Biol., 55, pp. 691-695; Ahmad, V.U., Noorwala, M., Mohammad, F.V., Sener, B., Aftab, K., Symphytoxide A, a triterpenoid saponin from the roots of Symphytum officinale (1993) Phytochemistry, 32, pp. 1003-1006; Haa, D., Abou-Mandour, A.A., Blaschek, W., Franz, G., Czygan, F.C., He influence of phytohormones on growth, organ differentiation and fructan production in callus of Symphytum officinale L (1991) Plant Cell Rep, 10, pp. 421-424; Abou-Mandour, A.A., Czygan, F.C., Haa, D., Franz, G., Fructan synthesis in tissue cultures of Symphytum officinale L.: Initiation, differentiation, and metabolic activity (1987) Planta Med, 53, pp. 482-487; Mohammad, F.V., Noorwala, M., Ahmad, V.U., Sener, B., A bidesmosidic hederagenin hexasaccharide from the roots of Symphytum officinale (1995) Phytochemistry, 40, pp. 213-218; Yunusova, S.G., Lyashenko, S.S., Fedorov, N.I., Fedorov, N.I., Denisenko, O.N., Lipids and lipophilic constituents of comfrey (Symphytum officinale L.) seeds (2017) Pharm. Chem. J., 50, pp. 728-731; Grabias, B., Swiatek, L., Phenolic acids in Symphytum officinale L (1998) Pharmaceutical and Pharmacological Letters, 8, pp. 81-83; Ulubelen, A., �cal, F., Alkaloids and other compounds of Symphytum tuberosum (1977) Phytochemistry, 16, pp. 499-500; Culvenor, C.C.J., Edgar, J.A., Frahn, J.L., Smith, L.W., The alkaloids of Symphytum � uplandicum (Russian comfrey) (1980) Aust. J. Chem., 33, pp. 1105-1113; Tamariz, J., Burgueo-Tapia, E., Vzquez, M.A., Delgado, F., Pyrrolizidine alkaloids (2018) The Alkaloids. Chem. Biol., 80, pp. 1-314; Chou, M.W., Fu, P.P., Formation of DHP-derived DNA adducts in vivo from dietary supplements and Chinese herbal plant extracts containing carcinogenic pyrrolizidine alkaloids (2006) Toxicol Ind Health, 22, pp. 321-327; Gomes, M.F.P.L., Massoco, C.O., Xavier, J.G., Bonamin, L.V., Comfrey (Symphytum officinale L.) and experimental hepatic carcinogenesis: A short-term carcinogenesis model study (2010) eCAM, 7, pp. 197-202; Mutterlein, R., Arnorld, C.G., Investigations concerning the content and the pattern of pyrrolizidine alkaloids in Symphytum officinale L. (comfrey) (1993) Pharm Ztg Wiss, 138, pp. 119-125; Couet, C.E., Crews, C., Hanley, A.B., Analysis, separation, and bioassay of pyrrolizidine alkaloids from comfrey (Symphytum officinale) (1996) Nat Toxins, 4, pp. 163-167; Oberlies, N.H., Kim, N.C., Brine, D.R., Collins, B.J., Handy, R.W., Sparacino, C.M., Wani, M.C., Wall, M.E., Analysis of herbal teas made from the leaves of comfrey (Symphytum officinale): Reduction of N-oxides results in order of magnitude increases in the measurable concentration of pyrrolizidine alkaloids (2004) Public Health Nutr, 7, pp. 919-924; Savi?, V.L., Nikoli?, V.D., Arsi?, I.A., Stanojevi?, L.P., Najman, S.J., Stojanovi?, S., Mladenovi?-Ranisavljevi?, I., Comparative study of the biological activity of allantoin and aqueous extract of the comfrey root (2015) Phytother. Res., 29, pp. 1117-1122; Al-Nimer, M.S., Wahbee, Z., Ultraviolet light assisted extraction of flavonoids and allantoin from aqueous and alcoholic extracts of Symphytum officinale (2017) J. Intercult. Ethnopharmacol., 6, pp. 280-285; Becker, L.C., Bergfeld, W.F., Belsito, D.V., Klaassen, C.D., Marks, J.G., Shank, R.C., Slaga, T.J., Andersen, F.A., Final report of the safety assessment of allantoin and its related complexes (2010) Int. J. Toxicol., 29, pp. 84S-97S; Alkan, F.U., Anlas, C., Ustuner, O., Bak?rel, T., Sari, A.B., Antioxidant and proliferative effects of aqueous and ethanolic extracts of Symphytum officinale on 3T3 Swiss albino mouse fibroblast cell line (2014) Asian J. Plant Sci. Res., 4, pp. 62-68; Rode, D., Comfrey toxicity revisited (2002) Trends Pharmacol. Sci., 23, pp. 497-499; Merlani, M., Barbakadze, V., Amiranashvili, L., Gogilashvili, L., Yannakopoulou, E., Papadopoulos, K., Chankvetadze, B., Enantioselective synthesis and antioxidant activity of 3-(3,4-dihydroxyphenyl)-glyceric acid-basic monomeric moiety of a biologically active polyether from Symphytum asperum and S. Caucasicum (2010) Chirality, 22, pp. 717-725; Kucera, M., K�lal, J., Polesna, Z., Effects ofSymphytum ointment on muscular symptoms and functional locomotor disturbances (2000) Adv. Ther., 17, pp. 204-211; Schmid, K., Ivemeyer, S., Vogl, C., Klarer, F., Meier, B., Hamburger, M., Walkenhorst, M., Traditional use of herbal remedies in livestock by farmers in 3 Swiss cantons (Aargau, Zurich, Schaffhausen) (2006) Forschende Komplementarmedizin, 19, pp. 125-136. , 2012, CrossRef PubMed; Neagu, E., Moroeanu, V., Radu, G., Concentration of Symphytum officinale extracts with cytostatic activity by tangential flow ultrafiltration (2008) Roum. Biotechnol. Lett., 13, pp. 4008-4013; Staiger, C., Comfrey root: From tradition to modern clinical trials (2013) Wien Med Wochenschr, 163, pp. 58-64; De Albuquerque, U.P., Monteiro, J.M., Ramos, M.A., De Amorim, E.L., Medicinal and magic plants from a public market in northeastern Brazil (2007) J. Ethnopharmacol., 110, pp. 76-91; Aceves-Avila, F.J., Medina, F., Fraga, A., Herbal therapies in rheumatology: The persistence of ancient medical practices (2001) Clin. Exp. Rheumatol., 19, pp. 177-183; Smith, N., Shin, D.B., Brauer, J.A., Mao, J., Gelfand, J.M., Use of complementary and alternative medicine among adults with skin disease: Results from a national survey (2009) J. Am. Acad. Dermatol., 60, pp. 419-425; Petkeviciute, Z., Savickiene, N., Savickas, A., Bernatoniene, J., Simaitiene, Z., Kalveniene, Z., Lazauskas, R., Mekas, T.A., Urban ethnobotany study in Samogitia region, Lithuania (2010) J. Med. Plants Res., 4, pp. 064-071; Cavero, R.Y., Akerreta, S., Calvo, M.I., Pharmaceutical ethnobotany in Northern Navarra (Iberian Peninsula) (2011) J. Ethnopharmacol., 133, pp. 138-146; Cavero, R.Y., Calvo, M.I., Medicinal plants used for musculoskeletal disorders in Navarra and their pharmacological validation (2015) J. Ethnopharmacol., 168, pp. 255-259; Sharifi-Rad, M., Nazaruk, J., Polito, L., Morais-Braga, M., Rocha, J., Coutinho, H., Salehi, B., Del, M.M.C., Matricaria genus as a source of antimicrobial agents: From farm to pharmacy and food applications (2018) Microbiol. Res., 215, pp. 76-88; Kapil, A.J.I.J., The challenge of antibiotic resistance: Need to contemplate (2005) Ind. J. Med. Res., 121, pp. 83-91; Lewis, K., Ausubel, F.M.J.N., Prospects for plant-derived antibacterials (2006) Nature Biotechnol, 24, p. 1504; Leach, M., A critical review of natural therapies in wound management (2004) Ostomy/Wound Manag, 50, pp. 36-40; Sharifi-Rad, J., Sharifi-Rad, M., Salehi, B., Iriti, M., Roointan, A., Mnayer, D., Soltani-Nejad, A., Afshari, A., In vitro and in vivo assessment of free radical scavenging and antioxidant activities of Veronica persica Poir (2018) Cell. Mol. Biol. (Noisy-Le-GrandFr.), 64, pp. 57-64; Koll, R., Buhr, M., Dieter, R., Pabst, H., Predel, H.-G., Petrowicz, O., Giannetti, B., Staiger, C., Efficacy and tolerance of a comfrey root extract (Extr. Rad. Symphyti) in the treatment of ankle distorsions: Results of a multicenter, randomized, placebo-controlled, double-blind study (2004) Phytomedicine, 11, pp. 470-477; Salehi, B., Valussi, M., Jugran, A.K., Martorell, M., Ramrez-Alarcn, K., Stojanovi?-Radi?, Z.Z., Antolak, H., Sharifi-Rad, M., Nepeta species: From Farm to Food applications and Phytotherapy (2018) Trends Food Sci. Technol., 80, pp. 104-122; (2015) WHO Estimates of the Global Burden of Foodborne Diseases. 2007-2015, , WHO. World Health Organization: Geneva, Switzerland; Scientific Opinion on the re-evaluation of ascorbic acid (E 300), sodium ascorbate (E 301) and calcium ascorbate (E 302) as food additives (2015) Efsa J, 13, p. 4087. , EFSA; (2018) The Re-'E'Valuation of Europe's Food Additives, , https://www.efsa.europa.eu/en/press/news/120130b, EFSA, T.r.-E.o.E.s.f.a.A.i.S, Available online: accessed on 1 September 2018; Lee, N.K., Paik, H.D., Status, antimicrobial mechanism, and regulation of natural preservatives in livestock food systems (2016) Korean J. Food Sci. Anim. Resour., 36, pp. 547-557; Settanni, L., Corsetti, A., Application of bacteriocins in vegetable food biopreservation (2008) Int. J. Food Microbiol., 121, pp. 123-138; MacDonald, R., Reitmeier, C., (2017) Understanding Food Systems: Agriculture, Food Science, and Nutrition in the United States, , Academic Press: Cambridge, MA, USA; Carocho, M., Morales, P., Ferreira, I.C.F.R., Antioxidants: Reviewing the chemistry, food applications, legislation and role as preservatives (2018) Trends Food Sci. Technol., 71, pp. 107-120; Wedzicha, B.L., (2013) PRESERVATIVES | Classifications and Properties; Sharifi-Rad, J., Soufi, L., Ayatollahi, S., Iriti, M., Sharifi-Rad, M., Varoni, E.M., Shahri, F., Kuhestani, K., Anti-bacterial effect of essential oil from Xanthium strumarium against Shiga toxin-producing Escherichia coli (2016) Cell. Mol. Biol., 62, pp. 69-74; Erdemoglu, N., Ozkan, S., Tosun, F., Alkaloid profile and antimicrobial activity of Lupinus angustifolius L. Alkaloid extract (2007) Phytochem. Rev., 6, pp. 197-201; Morteza-Semnani, K., Amin, G., Shidfar, M., Hadizadeh, H., Shafiee, A., Antifungal activity of the methanolic extract and alkaloids of Glaucium oxylobum (2003) Fitoterapia, 74, pp. 493-496; Slobodnkov, L., KoSt'lov, D., Labudov, D., Kotulov, D., Kettmann, V., Antimicrobial activity of Mahonia aquifolium crude extract and its major isolated alkaloids (2004) Phytother. Res.: Int. J. Devoted Pharmacol. Toxicol. Eval. Nat. Prod. Deriv., 18, pp. 674-676; Wuilloud, J.C., Gratz, S.R., Gamble, B.M., Wolnik, K.A., Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry (2004) Analyst, 129, pp. 150-156; Ma, C., Liu, Y., Zhu, L., Ji, H., Song, X., Guo, H., Yi, T., Determination and regulation of hepatotoxic pyrrolizidine alkaloids in food: A critical review of recent research. Food and chemical toxicology (2018) Food Chem. Toxicol., 119, pp. 50-60; Neuman, M.G., Cohen, L., Opris, M., Nanau, R.M., Jeong, H., Hepatotoxicity of pyrrolizidine alkaloids (2015) J. Pharm. Pharm. Sci., 18, pp. 825-843; Kartal, M., Kurucu, S., Choudary, M.I., Antifungal activities of different extracts and echimidine-N-oxide from Symphytum sylvaticum Boiss subsp. Sepulcrale (Boiss. & Bal.) Greuter & Burdet var. Sepulcrale (2001) Turk. J. Med Sci., 31, pp. 487-492; Savi?, V.L., Savi?, S.R., Nikoli?, V.D., Nikoli?, L.B., Najman, S.J., Lazarevi?, J.S., ?or?evi?, A.S., The identification and quantification of bioactive compounds from the aqueous extract of comfrey root by UHPLC-DAD-HESI-MS method and its microbial activity (2015) Hem. Ind., 69, pp. 1-8; Sumathi, S., Kumar, S.S., Bai, A., Glory, L., Evaluation of Phytochemical Constituents and Antibacterial Activities of Symphytum officinale L (2011) J. Pure Appl. Microbiol., 5, pp. 323-328; Sumathi, S., Antibacterial Activity of the plant extract of Symphytum officinale L. Against selected pathogenic bacteria (2016) Int. J. Res. Pharm. Sci., 2, pp. 92-94; Woods-Panzaru, S., Nelson, D., McCollum, G., Ballard, L.M., Millar, B.C., Maeda, Y., Goldsmith, C.E., Rao, J.R., An examination of antibacterial and antifungal properties of constituents described in traditional Ulster cures and remedies (2009) Ulst. Med J., 78, pp. 13-15; Barbakadze, V., Van Den Berg, A., Beukelman, C., Kemmink, J., Van Ufford, H.Q., Poly [3-(3, 4-dihydroxyphenyl) glyceric acid] from Symphytum officinale roots and its biological activity (2009) Chem. Nat. Compd., 45, pp. 6-10; Sharifi-Rad, M., Fokou, P., Sharopov, F., Martorell, M., Ademiluyi, A., Rajkovic, J., Salehi, B., Sharifi-Rad, J.J.M., Antiulcer agents: From plant extracts to phytochemicals in healing promotion (2018) Molecules, 23, p. 1751; Prakash, A.M., Sharifi-Rad, M., Shariati, M., Mabkhot, Y., Al-Showiman, S., Rauf, A., Salehi, B., Sharifi-Rad, J., Bioactive compounds and health benefits of edible Rumex species-A review (2018) Cell. Mol. Biol. (Noisy-Le-GrandFr.), 64, pp. 27-34; Prakash Mishra, A., Saklani, S., Salehi, B., Parcha, V., Sharifi-Rad, M., Milella, L., Iriti, M., Srivastava, M., Satyrium nepalense, a high altitude medicinal orchid of Indian Himalayan region: Chemical profile and biological activities of tuber extracts (2018) Cell. Mol. Biol. (Noisy-Le-GrandFr.), 64, pp. 35-43; Abdolshahi, A., Naybandi-Atashi, S., Heydari-Majd, M., Salehi, B., Kobarfard, F., Ayatollahi, S., Ata, A., Montanari, C., Antibacterial activity of some Lamiaceae species against Staphylococcus aureus in yoghurt-based drink (Doogh) (2018) Cell. Mol. Biol. (Noisy-Le-GrandFr.), 64, pp. 71-77; (2014) WHO Traditional Medicine Strategy 2014-2023, , http:llapps.who.int7irislbitstream710665l92455l1l9789241506090eng.pdf?ua, World Health Organization: Available online: accessed on 18 June 2019; Knaak, N., Dias Da Silva, L., Finger Andreis, T., Mariana Fiuza, L., Chemical characterization and anti-fungal activity of plant extracts and essential oils on the Bipolaris oryzae and Gerlachia oryzae phytopathogens (2013) Australas. Plant Pathol., 42, pp. 469-475; Rocha, R., Eleutrio Da Luz, D., Engels, C., Pileggi, S., De Souza Jaccoud Filho, D., Matiello, R., Pileggi, M., Selection of endophytic fungi from comfrey (Symphytum officinale L.) for in vitro biological control of the phytopathogen Sclerotinia sclerotiorum (Lib.) (2009) Braz. J. Microbiol., 40, pp. 73-78; Cvetkovic, D., Stanojevi?, L., Kundakovi?, T., Zlatkovic, S., Nikoli?, G., Antioxidant and antimicrobial activity of a new generation phyto-gel (2015) Adv. Technol., 4, pp. 11-18; Bouzada, M.L.M., Fabri, R.L., Nogueira, M., Konno, T.U.P., Duarte, G.G., Scio, E., Antibacterial, cytotoxic and phytochemical screening of some traditional medicinal plants in Brazil (2009) Pharm. Biol., 47, pp. 44-52; Borchardt, J.R., Wyse, D.L., Sheaffer, C.C., Kauppi, K.L., Ehlke, R.G.F.N., Biesboer, D.D., Bey, R.F., Antimicrobial activity of native and naturalized plants of Minnesota and Wisconsin (2008) J. Med. Plants Res., 2, pp. 098-110; Onofre, N.A., Magalhes, L.P.M., Amaral, J.P.D., Yara, R., Lima, C.S.A., Sena, K.X.F.R., Antimicrobial activity of plants used in manufacture of traditional phytotherapics in community centres of Recife metropolitan region (2016) Exp. Pathol. Health Sci., 8, pp. 23-24; Savic, V., Nikolic, V., Stanojevic, L., Ilic, D., Stankovic, B., Extraction kinetics and antioxidant activity of an aqueous extract from comfrey root (Symphytum officinale L.) (2012) Adv. Technol., 1, pp. 41-47; Dolganiuc, A., Radu, L., Olinescu, A., The effect of products of plant and microbial origin on phagocytic function and on the release of oxygen free radicals by mouse peritoneal macrophages (1997) Bacteriol Virusol Parazitol Epidemiol, 42, pp. 65-69; Repetto, M., Llesuy, S., Antioxidant properties of natural compounds used in popular medicine for gastric ulcers (2002) Braz. J. Med Biol. Res., 35, pp. 523-534; Barthomeuf, C., Debiton, E., Barbakadze, V., Kemertelidze, E., Evaluation of the dietetic and therapeutic potential of a high molecular weight hydroxycinnamate-derived polymer from Symphytum asperum Lepech. Regarding its antioxidant, antilipoperoxidant, antiinflammatory, and cytotoxic properties (2001) J. Agric. Food Chem., 49, pp. 3942-3946; Oktay, M., Yildirim, A., Bilaloglu, V., Glin, I., Antioxidant activity of different parts of isgin (Rheum ribes L.) (2007) Asian J. Chem., 19, p. 3047; Paun, G., Neagu, E., Litescu, S.C., Rotinberg, P., Radu, G.L., Application of membrane processes for the concentration of Symphytum officinale and Geranium robertianum extracts to obtain compounds with high anti-oxidative activity (2012) J. Serb. Chem. Soc., 77; Thring, T.S., Hili, P., Naughton, D.P., Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants (2009) Bmc Complementary Altern. Med., 9, p. 27; Nossa Gonzlez, D.L., Prez, T., Vernica, Y., Nez, R., Elas, W., Determination of polyphenols and antioxidant activity of polar extracts of comfrey (Symphytum officinale L) (2016) Rev. Cuba. De Plantas Med., 21, pp. 125-132; Badridze, G., Kacharava, N., Chkhubianishvili, E., Rapava, L., Kikvidze, M., Chigladze, L., Chanishvili, S., Content of antioxidants in leaves of some plants of Tbilisi environs (2013) Bull. Georg. Natl. Acad. Sci, 7; Smith, D.B., Jacobson, B.H., Effect of a blend of comfrey root extract (Symphytum officinale L.) and tannic acid creams in the treatment of osteoarthritis of the knee: Randomized, placebo-controlled, double-blind, multiclinical trials (2011) J. Chiropr. Med., 10, pp. 147-156; Liu, C., Chang, J., Zhang, L., Zhang, J., Li, S., Purification and antioxidant activity of a polysaccharide from bulbs of Fritillaria ussuriensis Maxim (2012) Int. J. Biol. Macromol., 50, pp. 1075-1080; Pielesz, A., Vibrational spectroscopy and electrophoresis as a golden means in monitoring of polysaccharides in medical plant and gels (2012) Spectrochim. Acta-Part A: Mol. Biomol. Spectrosc., 93, pp. 63-69; Duan, M., Shang, H., Chen, S., Li, R., Wu, H., Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques (2018) Int. J. Biol. Macromol., 115, pp. 876-882; Barbakadze, V.V., Kemertelidze, E.P., Targamadze, I.L., Shashkov, A.S., Usov, A.I., Poly[3-(3,4-dihydroxyphenyl)glyceric acid]: A new biologically active polymer from two comfrey species Symphytum asperum and S. Caucasicum (Boraginaceae) (2002) Russ. J. Bioorganic Chem., 28, pp. 326-330; Matcovschi, C., Calistru, Z., Cojocaru, M., Valcu, S., Symphytum officinale (L) gaertn - A prospective hepatoprotective and hepatoregenerative plant (1995) Pharmacol. Res., 31, p. 91; Oberbaum, M., Yakovlev, E., Kaufman, D., Shoshan, S., Effect of Arnica Montana and Symphytum officinalis on bone healing in Guinea pigs (1994) Br. Homeopath. J., 83, p. 90; Araujo, L.U., Reis, P.G., Barbosa, L.C., Saude-Guimaraes, D.A., Grabe-Guimaraes, A., Mosqueira, V.C., Carneiro, C.M., Silva-Barcellos, N.M., In vivo wound healing effects of Symphytum officinale L. Leaves extract in different topical formulations (2012) Pharmazie, 67, pp. 355-360; Kommission, E., Monographie Symphyti radix (Beinwellwurzel) (1990) Bundesanzeiger, p. 318; Vostinaru, O., Conea, S., Mogosan, C., Toma, C., Borza, C., Vlase, L., Anti-inflammatory and antinociceptive effect of Symphytum officinale root (2017) Rom. Biotechnol. Lett.; Hiermann, A., Writzel, M., Antiphlogistic glycopeptide from the roots of Symphytum officinale (1998) Pharm. Pharmacol. Lett., 8, pp. 154-157; Grigore, A., Pirvu, L., Bubueanu, C., Minerva, P., Rasit, I., Influence of chemical composition on the antioxidant and anti-inflammatory activity of Rosmarinus officinalis extracts (2015) Rom. Biotechnol. Lett., 20, pp. 10047-10054; Schmidtke-Schrezenmeier, G., The efficacy of a phytotherapeutic agent in the treatment of non-activated osteoarthritis of the knee [Behandlung der nichtaktivierten Gonarthrose: Besserung durch ein Phytotherapeutikum] (1992) Therapiewoche, 42, pp. 1322-1325; Grube, B., Grnwald, J., Krug, L., Staiger, C., Efficacy of a comfrey root (Symphyti offic. Radix) extract ointment in the treatment of patients with painful osteoarthritis of the knee: Results of a double-blind, randomised, bicenter, placebo-controlled trial (2007) Phytomedicine, 14, pp. 2-10; Laslett, L., Quinn, S., Darian-Smith, E., Kwok, M., Fedorova, T., Krner, H., Steels, E., Jones, G., Treatment with 4Jointz reduces knee pain over 12 weeks of treatment in patients with clinical knee osteoarthritis: A randomised controlled trial (2012) Osteoarthr. Cartil., 20, pp. 1209-1216; Giannetti, B.M., Staiger, C., Bulitta, M., Predel, H.-G., Efficacy and safety of comfrey root extract ointment in the treatment of acute upper or lower back pain: Results of a double-blind, randomised, placebo controlled, multicentre trial (2009) Br. J. Sports Med., 44, pp. 637-641; Pabst, H., Schaefer, A., Staiger, C., Junker-Samek, M., Predel, H.G., Combination of comfrey root extract plus methyl nicotinate in patients with conditions of acute upper or low back pain: A multicentre randomised controlled trial (2013) Phytother. Res., 27, pp. 811-817; Petersen, G., Lorkowski, G., Kasper, F., Gottwald, R., Lcker, P., Anti-inflammatory activity of a pyrrolizidine alkaloid-free extract of roots of Symphytum officinale in humans (1993) Planta Med, 59, pp. A703-A704; Predel, H.-G., Giannetti, B., Koll, R., Bulitta, M., Staiger, C., Efficacy of a Comfrey root extract ointment in comparison to a Diclo-fenac gel in the treatment of ankle distortions: Results of an observer-blind, randomized, multicenter study (2005) Phytomedicine, 12, pp. 707-714; Ku?era, M., Barna, M., Horek, O., Kovrikov, J., Ku?era, A., Efficacy and safety of topically applied Symphytum herb extract cream in the treatment of ankle distortion: Results of a randomized controlled clinical double-blind study (2004) Wien. Med. Wochenschr., 154, pp. 498-507; Kucera, M., Barna, M., Horcek, O., Klal, J., Kucera, A., Hladkova, M., TopicalSymphytum herb concentrate cream against myalgia: A randomized controlled double-blind clinical study (2005) Adv. Ther., 22, pp. 681-692; Barna, M., Kucera, A., Hladcova, M., Kucera, M., Der wundheilende Effekt einer Symphytum-Herba-Extrakt-Creme (Symphytum uplandicum Nyman): Ergebnisse einer randomisierten, kontrollierten Doppelblindstudie (2007) Wien. Med. Wochenschr., 157, pp. 569-574; Grnwald, J., Bitterlich, N., Nauert, C., Schmidt, M., Anwendung und Vertrglichkeit von Beinwellcreme (Symphyti herba) bei Kindern mit akuten stumpfen Traumen (2010) Z.

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