Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis
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Date
2022-03
Authors
El A Helal, Thanaa
El Abdel Wahab, Hoda E
Saber, Sally M
Abdelaaty, Waleed H
Eltabbakh, Mohamed M
Aref, Ahmed M
Dawood, Mohamed H
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Makerere University, Medical School
Series Info
African Health Sciences,;Vol 22 Issue 1, March, 2022
;Volume 22, Issue 1, Pages 602 - 6102022
;Volume 22, Issue 1, Pages 602 - 6102022
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Abstract
Background/Aim: Ulcerative Colitis (UC) is an inflammatory bowel disease which is common in many areas of the world including Egypt. A lot of controversy regarding the pathogenesis of UC exist. The current study is an attempt to detect some pathogenic bacteria in UC patients.
Materials and methods: Endoscopic colonic biopsies obtained from 40 patients with ulcerative colitis and 20 controls were analyzed by means of real-time PCR technique for the presence of Clostridium difficile, Helicobacter Pylori (H. pylori) and pathogenic Escherichia Coli (E. coli) which are positive for KPC and/or OXA-48.
Results: All patients and control samples were negative for Clostridium difficile. Three of the 40 patient samples (7.5%) and none of the 20 controls were positive for H. pylori with no significant difference between the two groups. KPC-positive E. coli were detected in 11 of the 40 patients (27.5%) and in none of the controls with a significant difference between the two groups (P=0.01). All patients and control samples were negative for OXA-48 positive E. coli.
Conclusion: Although this study does not support the claim that Clostridium difficile and/or H. pylori have a role in UC, it greatly suggests that pathogenic E. coli may be involved in one way or another in the course of UC.
Description
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Keywords
Ulcerative colitis, Colonic biopsies, Clostridium difficile, H. pylori, E. coli