Browsing by Author "Yehia A.M."

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Chemometrics for resolving spectral data of cephalosporines and tracing their residue in waste water samples
(Elsevier B.V., 2019) Yehia A.M.; Elbalkiny H.T.; Riad S.M.; Elsaharty Y.S.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini 13 Street; Cairo; 11562; Egypt; Chemistry Department; School of Pharmacy and Pharmaceutical Industries; Badr University in Cairo; Badr City; Cairo 11829; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th of October City; 11787; Egypt
Chemometrics approaches have been used in this work to trace cephalosporins in aquatic system. Principal component regression (PCR), partial least squares (PLS), multivariate curve resolution-alternating least squares (MCR-ALS), and artificial neural networks (ANN) were compared to resolve the severally overlapped spectrum of three selected cephalosporins; cefprozil, cefradine and cefadroxil. The analytical performance of chemometric methods was compared in terms of errors. Artificial neural networks provide good recoveries with lowest error. Satisfactory results were obtained for the proposed chemometric methods whereas ANN showed better analytical performance. The qualitative meaning in MCR-ALS transformation provided very well correlations between the pure and estimated spectra of the three components. This multivariate processing of spectrophotometric data could successfully detect the studied antibiotics in waste water samples and compared favorably to alternative costly chromatographic methods. � 2019
Comparison of Two Stability-Indicating Chromatographic Methods for the Determination of Mirabegron in Presence of Its Degradation Product
(Friedr. Vieweg und Sohn Verlags GmbH, 2017) Yehia A.M.; Sami I.; Riad S.M.; El-Saharty Y.S.; Department of Analytical Chemistry; Faculty of Pharmacy; Cairo University; Kasr El-Aini Street; Cairo; 11562; Egypt; Pharmaceutical Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts; 6 October City; 11787; Egypt
Mirabegron is a novel ?3-adrenoceptor agonist containing an amide group. It was subjected to stress conditions of acidic and alkaline hydrolyses. The hydrolytic degradation product was isolated and its structure was confirmed using mass and IR spectrometry. Two stability-indicating chromatographic methods have been proposed for the determination of mirabegron. TLC method was applied using silica gel as stationary phase and chloroform methanol ammonia (9.0:1.0:0.1 by volume) as the mobile phase, and chromatograms were scanned at 250. nm. Accurate determination of the drug was achieved over the concentration range of 2. 1. g per band. In addition, an isocratic HPLC method was developed on Agilent C18 column (150. mm. נ4.5.mm I.D., particle size 5.m) using ethanol-phosphate buffer pH 2.5 (30:70, by volume) as a mobile phase with flow rate of 1.mL.min?1.The intact drug was detected at 255.nm with running time less than 5.min. Mirabegron was determined accurately in a concentration range of 1.25.g.mL?1. The proposed chromatographic methods were applied successfully for the assay of mirabegron in pharmaceutical dosage form and both methods were validated as per the International Conference on Harmonization guidelines and statistically compared with a reported gradient HPLC method. 2016, Springer-Verlag Berlin Heidelberg.
Derivative constant wavelength synchronous fluorescence spectrometry for the simultaneous detection of cefadrine and cefadroxil in water samples
(Elsevier B.V., 2020) Elbalkiny H.T.; Yehia A.M.; Riad S.M.; Elsaharty Y.S.; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th October City; 11787; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini 13 Street; Cairo; 11562; Egypt; Chemistry Department; School of Pharmacy and Pharmaceutical Industries; Badr University in Cairo; Badr City; Cairo 11829; Egypt
A sensitive accurate spectrofluorimetric technique was developed to detect cefadroxil and cefradine traces in water samples simultaneously, by applying a procedure based on the formation of hydrolysis products corresponding to these compounds by sodium hydroxide (1 N NaOH) treatment. The conventional and the synchronous fluorescence spectra of these hydrolyzed products were totally overlapped making resolving of this mixture impossible. The second-derivative constant-wavelength synchronous fluorescence spectra allowed their detection simultaneously in a single scan after experimental conditions optimization, which was measured at 390 nm and 379 nm for cefadroxil and cefradine, respectively at ?? = 30.0. The calibration curves between derivative synchronous fluorescence intensity and drugs concentration showed suitable linear correlation in the range of 0.1 to 5 ?g.mL? 1 for cefadroxil and 0.5�10 ?g.mL? 1 for cefradine. The proposed fluorimetric method is superior in being simple, environmental friendly and cost effective in comparison to the previously published reported methods. � 2019 Elsevier B.V.
Monitoring and optimization of diclofenac removal by adsorption technique using in-line potentiometric analyzer
(Elsevier Inc., 2019) Yehia A.M.; Elbalkiny H.T.; Riad S.M.; Elsaharty Y.S.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini 13 Street; Cairo; 11562; Egypt; Chemistry Department; School of Pharmacy and Pharmaceutical Industries; Badr University in Cairo; Badr City; Cairo 11829; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th October City; 11787; Egypt
Diclofenac (DICLO) is one of the most prescribed non-steroidal anti-inflammatory drugs worldwide. The presence of DICLO residues in aquatic environment either from industrial or hospital wastewater has harmful effects on many organisms. In this contribution, the adsorption of DICLO on granulated active carbon and mesoporous silica nanoparticles was optimized in order to remove those residues from water. The tracking of DICLO was done using in-line potentiometric ion-selective electrodes (ISE) through the whole experiments. The constructed electrode allows tracking of DICLO residues in real time. A central composite design was applied, inspecting the pH, initial concentration, adsorbent loading concentration and adsorbent type effects on the adsorption process, the results showed that the pH effect was most significant; pH 5 gave best results. The adsorption kinetics of these adsorbents have been investigated and the results indicated that the adsorption process followed the pseudo-second-order kinetic model. The adsorption isotherm data were analyzed by both Langmuir and Freundlich models and later provides better fit of the experimental data. The removal efficiency was about ~89% upon applying the optimum set of experimental conditions in time < 60 min. In-line monitoring is considered a green protocol, that should be carried out also at the pharmaceutical industry scale due to the high selectivity, lack of harmful waste generation and minimal solvent use. � 2019 Elsevier B.V.
Potentiometric diclofenac detection in wastewater using functionalized nanoparticles
(Elsevier Inc., 2019) Elbalkiny H.T.; Yehia A.M.; Riad S.M.; Elsaharty Y.S.; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th October City; 11787; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini 13 Street; Cairo; 11562; Egypt; Chemistry Department; School of Pharmacy and Pharmaceutical Industries; Badr University in Cairo; Badr City; Cairo; 11829; Egypt
Ion selective electrodes for diclofenac monitoring in both pharmaceutical wastewater and dosage form were described; that are considered environmental friendly analytical method. The sensors development depended on comparative performance evaluation of membranes that were based on functionalized magnetite nanoparticles with the classical sensors; this approach provided that nanoparticles in the inner solution of sensor membrane were highly dispersed and coated with ionophore to enhance a complete ion-pairing interaction between the ionophore and the analyte. The optimum membrane was that containing ??cyclodextrin coupled with magnetite ferric oxide as inner filling solution, dibutylphthalate as plasticizer and crystal violet as ion exchanger in poly (vinylchloride) matrix. This sensor (CV-Fe-?-CD) exhibited high sensitivity, Nernstian slope of the calibration curve, as well as fast, stable response and good selectivity. The sensor exhibits a Nernstian slope of ?58.7 � 1 mV/decade over the concentration range 1.0 � 10?7 to 1.0 � 10?2 M of Diclofenac with a minimal limit of detection of 1.1 � 10?7 M. The electrode showed a good potentiometric selectivity for diclofenac with respect to a number of interfering ions and organic species. The membrane sensor was successfully applied for the determination of diclofenac in wastewater samples and dosage form without sample pretreatment steps prior to its analysis. � 2018 Elsevier B.V.
Qualitative and quantitative chemometry as stability-indicating methods for determination of dantrolene sodium and paracetamol
(Bentham Science Publishers B.V., 2018) Yehia A.M.; Sami I.; Riad S.M.; El-Saharty Y.S.; Cairo University; Faculty of Pharmacy; Department of Analytical Chemistry; Kasr El-Aini Street; Cairo; ET 11562; Egypt; October University for Modern Sciences and Arts; Faculty of Pharmacy; Pharmaceutical Analytical Chemistry Department; 6 October City; 11787; Egypt
Background: Simultaneous determination of binary mixtures with simple and cost effective analysis is always of interest. Progressive advancement in chemometrics enables spectral resolution of drugs in the presence of their degradation products or impurities. Methods and Result: Three stability indicating chemometric methods are applied for the simultaneous determination of Dantrolene sodium (DNT) and Paracetamol (PAR). Partial Least Squares (PLS), Concentration Residuals Augmented Classical Least Squares (CRACLS) and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) were selected for that purpose. DNT and PAR were determined in the linearity range of (2 � 10 ?g mL-1) and (12 � 28 ?g mL-1), respectively, in the presence of their degradation products. The presented methods were compared for their qualitative and quantitative analyses and validated according to the ICH guidelines. Furthermore, statistical comparison between the results obtained by the proposed methods and the reported chromatographic method showed no significant differences. Conclusion: The proposed multivariate calibrations were accurate and specific for quantitative analysis of the studied components. Furthermore, CRACLS and MCR-ALS methods succeeded in both quantitative and qualitative of the studied components and their degradation products. � 2018 Bentham Science Publishers.
Removal and tracing of cephalosporins in industrial wastewater by SPE-HPLC: optimization of adsorption kinetics on mesoporous silica nanoparticles
(Springer, 2019) Elbalkiny H.T.; Yehia A.M.; Riad S.M.; Elsaharty Y.S.; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th of October City; 11787; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini 13 Street; Cairo; 11562; Egypt; Chemistry Department; School of Pharmacy and pharmaceutical industries; Badr University in Cairo; Badr City; Cairo 11829; Egypt
Cephalosporins are ?-lactam antibiotics which are considered the most widely used for humans and animals in many countries. Solid phase extraction was applied using Oasis HLB cartridges for tracing three cephalosporins (cefadroxil, cefprozil, and cefradine) in industrial wastewater samples. Sample extraction was followed by chromatographic analysis. The developed high-performance liquid chromatographic method was used for the determination of these selected cephalosporins. Separation was achieved on a Hypersil C18 column with an isocratic elution of acidified water (pH 2.5) to methanol to acetonitrile (82:12:6 per volume) at a flow rate of 1.5 mL min?1 and UV detection at 254 nm. Moreover, the proposed extraction and analysis procedures were applied to study the ability of mesoporous silica nanoparticles to remove cephalosporin residues from water by adsorption technique. A factorial design was applied to investigate the effect of pH, initial cephalosporins concentration, and adsorbent concentration on the adsorption process using removal percentage as a response. The prediction model fit well to experimental data based on statistical analysis. Adsorption kinetics and isotherm have been also studied, and the experimental results indicated that the adsorption process followed the pseudo-second-order kinetic model and Freundlich isotherm. � 2019, The Author(s).