Browsing by Author "Ramla, Dalila"

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Genetic diversity analysis of North Africa’s barley using SSR markers
(Elsevier, 2012) Ben Naceur, Amani; Chaabane, Ramzi; El-Faleh, Mouldi; Abdelly, Chedly; Ramla, Dalila; Nada, Ahmed; Sakr, Mahmoud
It was demonstrated that some North Africa barley accessions have diverse tolerance sources for abiotic stresses and a good nutritional quality, but the studies done were incomplete since they were realized separately in each country apart. To implement a more complete analysis, 31 barley accessions originated from North Africa (Algeria, Tunisia and Egypt) were analyzed using 11 SSR markers selected from the seven barley linkage groups for studying the genetic diversity among these chosen barley accessions. Over the 11 SSR markers, a total of 478 reproducible bands were scored with an average of 2.13 alleles/primer and the average polymorphism information content of 0.5. Genetic distance analysis of the 31 accessions showed a large genetic diversity and high number of different groups. The most accessions are clustered according to their eco-geographical origin, according to their pedigree and agronomic characters or according to the caryopsis character (hulled or naked caryopsis). This high number of obtained groups is an invaluable aid in crop improvement strategies and confirms the opinion suggesting that North Africa could be a secondary center of origin of barley. The various growing conditions and the multiple uses of barley in each country may be the cause of the large variability of the barley germplasm in each region.
Molecular Characterization of Tunisian Barley (Hordeum Vulgare L.) Genotypes using Microsatellites (SSRs) Markers
(European Journal of Scientific Research, 2009) Chaabane, Ramzi; El Felah, Mouldi; Ben Salah, Hammadi; Ben Naceur, M’Barek; Abdelly, Chedly; Ramla, Dalila; Nada, Ahmad; Saker, Mahmoud
High level of polymorphism make simple sequence repeats (SSRs) the molecular marker of choice for diversity analysis in plant species. In this study 18 simple sequence repeats (SSRs) markers were used to characterize six Tunisian barley varieties (Faïz, Manel, Martin, Rihane, Roho, and Tej) as well as six landraces from different growing regions in Tunisia (Djerba, Gabes, Jendouba, kairouan, Kebili, Kerkennah). Amplification of SSRs loci were obtained for 17 primer pairs and only 11 among them showed clear polymorphic patterns. These 11 primers produced a total of 31 alleles. The number of alleles per marker ranged from 1 to 5 with an average of 2.81 alleles per locus. The data generated by these 11 primers were sufficient to discriminate the analysed barley genotypes. The UPGMA cluster analysis of the similarity data grouped the 12 studied genotypes into two groups according to their spike type (two-row and six-row barley), except for Manel verity witch is a six row type but it clustered among the two row group. These results will be useful for barley germplasm management in terms of biodiversity protection and design of new crosses