Browsing by Author "Linscheid M.W."

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Acylated flavonol diglucosides from Ammania auriculata
(Verlag der Zeitschrift fur Naturforschung, 2015) Nawwar M.; Ayoub N.; El-Raey M.; Zaghloul S.; Hashem A.; Mostafa E.; Eldahshan O.; Lindequist U.; Linscheid M.W.; National Research Center; Department of Phytochemistry and Plant Systematics; El Bohooth Str.; P.O. 12622; Dokki; Cairo; Egypt; Faculty of Pharmacy; Department of Pharmacognosy; Ain-Shams University; Cairo; Egypt; October University for Modern Sciences and Arts; 6th October City; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Institute of Pharmacy; Pharmaceutical Biology; Ernst-Moritz-Arndt-Universit�t Greifswald; Greifswald; Germany; Laboratory of Applied Analytical and Environmental Chemistry; Department of Chemistry; Humboldt-Universit�t zu Berlin; Berlin; Germany
Chemical investigation of the extract of the whole Ammania auriculata plant resulted in the identification of 13 polyphenols, including the hitherto unknown flavonoids, kaempferol 3-O-?-(6?-galloylglucopyranoside)- 7-O-?-glucopyranoside, and its quercetin analogue. The structures of all isolates were elucidated by conventional methods, spectroscopic analysis, including 1D and 2D NMR, and by HRESI-MS as well.
Antitumor activity of Cuphea ignea extract against benzo(a)pyrene-induced lung tumorigenesis in Swiss Albino mice
(Taylor and Francis Ltd., 2019) Hassan S.K.; Mousa A.M.; El-Sammad N.M.; Abdel-Halim A.H.; Khalil W.K.B.; Elsayed E.A.; Anwar N.; Linscheid M.W.; Moustafa E.S.; Hashim A.N.; Nawwar M.; Department of Biochemistry; National Research Centre; Dokki; Cairo; Egypt; Department of Cell Biology; National Research Centre; Dokki; Cairo; Egypt; Bioproducts Research Chair; Zoology Department; College of Science; King Saud University; Riyadh; Saudi Arabia; Department of Chemistry of Natural and Microbial Products; National Research Centre; Dokki; Cairo; Egypt; Department of Pathology; National Cancer Institute; Cairo University; Cairo; Egypt; Laboratory of Applied Analytical and Environmental Chemistry; Humboldt-University; Berlin; Germany; October University of Modern Sciences and Arts; 6th October City; Egypt; Department of Phytochemistry and Plant Systematics; National Research Centre; Cairo; Egypt
Lung cancer has one of the highest mortality rates among various types of cancer and is the most frequent cancer in the world. The incidence of lung cancer is increasing rapidly, in parallel with an increased incidence of smoking. Effective chemoprevention may be an alternative strategy to control the incidence of lung cancer. Thus, the objective of current work was to ascertain the possible preventive and therapeutic efficacies of Cuphea ignea extract in a mouse model of lung tumorigenesis and its cytotoxicity toward the A549 human lung cancer cell line. Lung tumorigenesis was induced by the oral administration of benzo(a)pyrene (50 mg/kg b.w.) twice per week to Swiss albino mice for 4 weeks. Benzo(a)pyrene-treated mice were orally administered C. ignea (300 mg/kg body weight, 5 days/week) for 2 weeks before or 9 weeks after the first benzo(a)pyrene dose, for a total of 21 weeks. At the end of the administration period, various parameters were measured in the serum and lung tissues. The results revealed that the oral administration of benzo(a)pyrene resulted in increases in relative lung weight, serum levels of tumor markers (ADA, AHH, and LDH), and the inflammatory marker NF-?B, and a decreased total antioxidant capacity compared with the control. In addition, decreased levels of enzymatic and non-enzymatic antioxidants, with a concomitant increase in lipid peroxidation, metalloproteinases (MMP-2 and MMP-12), and the angiogenic marker VEGF were detected in lung tissues. Moreover, benzo(a)pyrene administration induced the upregulation of PKC?, COX-2, and Bcl-2 expression, with the downregulation of BAX and caspase-3 expression. C. ignea treatment alleviated all alterations in these parameters, which was further confirmed by the histopathological analysis of lung tissues. The findings of the current work provide the first verification of the preventive and therapeutic potentials of C. ignea extract against benzo(a)pyrene-induced lung tumorigenesis in mice. � 2019 The Authors
A coumarin with an unusual structure from Cuphea ignea, its cytotoxicity and antioxidant activities
(Govi-Verlag Pharmazeutischer Verlag GmbH, 2018) Moustafa E.S.; Swilam N.F.; Ghanem O.B.; Hashim A.N.; Nawwar M.A.; Lindequist U.; Linscheid M.W.; October University for Modern Sciences and Arts; 6th October City; Egypt; Pharmacognosy Department; Faculty of Pharmacy; British University; Egypt; Under Graduate Research Trainee; Faculty of Pharmacy; German University; Cairo; Egypt; Department of Phytochemistry and Plant Systematics; National Research Center; 14 El Tahrier Str.; Dokki; Cairo; 1361; Egypt; Pharmaceutical Biology; Institute of Pharmacy; Ernst-Moritz-Arndt-University; Greifswald; Germany; Laboratory of Applied Analytical and Environmental Chemistry; Humboldt-University; Berlin; Germany
Phenolic metabolite profiling using two dimensional paper chromatographic analysis (2 DPC) was used for assaying the complex mixture of phenolics of an aqueous ethanol aerial part extract of Cuphea ignea (Lytheraceae). A coumarin with a rare structure, namely, 7-hydroxy 3-methoxy coumarin 5-O-?-glucopyranoside was isolated from the investigated extract. The structure was elucidated by conventional methods and spectral analysis, including one and two dimensional NMR (1D and 2D NMR), as well as by interpretation of the spectra obtained by high resolution electrospray ionization mass technique (HRESIMS). The rare coumarin significantly inhibited reactive oxygen species production with an ED50 value of 6.31�1.64 ?g/ml and 5.78�0.66 ?g/ml as determined by the the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the oxygen radical absorption capacity (ORAC) assay respectively. The isolated coumarin presented a cytotoxic activity assessed by using the neutral red assay (NRU) against lung cancer cell line (H23) with IC50 of 40.38�2.75 ?g/ml. � 2018 Govi-Verlag Pharmazeutischer Verlag GmbH. All rights reserved.
Polyphenols in Ammania auriculata: Structures, antioxidative activity and cytotoxicity
(Govi-Verlag Pharmazeutischer Verlag GmbH, 2014) Nawwar M.A.; Youb N.A.; El-Raey M.A.; Zaghloul S.S.; Hashem A.M.; Mostafa E.S.; Eldahshan O.; Werner V.; Becker A.; Haertel B.; Lindequist U.; Linscheid M.W.; Department of Phytochemistry and Plant Systematics; National Research Center; Dokki Cairo; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Ain-Shams University; Cairo; Egypt; October University for Modern Sciences and Arts; 6th October City; Egypt; Institute of Pharmacy; Pharmaceutical Biology; Ernst-Moritz-Arndt-Universit�t; Greifswald; Mecklenburg-Vorpommern; Germany; Department of Chemistry; Laboratory of Applied Analytical and Environmental Chemistry; Humboldt -University; Berlin; Germany
Chemical and biological investigations of the extract of Ammania auriculata (Lytheraceae) resulted in the identification of eight polyphenols (1 - 8) for the first time from this plant, including the gallotannin, 2,3,6-tri-O-galloyl-(?,?)-4C1-glucopyranose (8), for which 1D and 2D-NMR spectra were recorded and assigned for the first time. The structures of all isolates (1 - 8) were elucidated by conventional methods, spectroscopic analysis, including 1D and 2D NMR, and by HR-ESIMS as well. All of the isolated compounds were evaluated for their antioxidant activities, determined by the DPPH and ORAC methods and for their cytotoxicity against the keratinocyte cell line HaCaT using the neutral red assay (NRU) and cell cycle analysis. Compounds 1, 3, 4, 5, and 6 significantly inhibited reactive oxygen species production with ED50 values between 3.22 and 9.79 ?g/ml. Compounds 1, 3, 4, and 5 showed cytotoxic activity against HaCaT cells with IC50 values between 30.7 and 84.1 ?g/ml. The new galloyl glucose (8) was found not cytotoxic. Ellagitannins, 2,3-hexahydroxy-(?/?)-glucopyranose (1) and 1-O-galloyl 2,3-hexahydroxy-(?)-glucopyranose (5) possess remarkable antioxidative and comparably weak cytotoxic activity.