Antitumor activity of Cuphea ignea extract against benzo(a)pyrene-induced lung tumorigenesis in Swiss Albino mice
Date
2019
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Taylor and Francis Ltd.
Elsevier Inc.
Elsevier Inc.
Series Info
Toxicology Reports
6
6
Scientific Journal Rankings
Abstract
Lung cancer has one of the highest mortality rates among various types of cancer and is the most frequent cancer in the world. The incidence of lung cancer is increasing rapidly, in parallel with an increased incidence of smoking. Effective chemoprevention may be an alternative strategy to control the incidence of lung cancer. Thus, the objective of current work was to ascertain the possible preventive and therapeutic efficacies of Cuphea ignea extract in a mouse model of lung tumorigenesis and its cytotoxicity toward the A549 human lung cancer cell line. Lung tumorigenesis was induced by the oral administration of benzo(a)pyrene (50 mg/kg b.w.) twice per week to Swiss albino mice for 4 weeks. Benzo(a)pyrene-treated mice were orally administered C. ignea (300 mg/kg body weight, 5 days/week) for 2 weeks before or 9 weeks after the first benzo(a)pyrene dose, for a total of 21 weeks. At the end of the administration period, various parameters were measured in the serum and lung tissues. The results revealed that the oral administration of benzo(a)pyrene resulted in increases in relative lung weight, serum levels of tumor markers (ADA, AHH, and LDH), and the inflammatory marker NF-?B, and a decreased total antioxidant capacity compared with the control. In addition, decreased levels of enzymatic and non-enzymatic antioxidants, with a concomitant increase in lipid peroxidation, metalloproteinases (MMP-2 and MMP-12), and the angiogenic marker VEGF were detected in lung tissues. Moreover, benzo(a)pyrene administration induced the upregulation of PKC?, COX-2, and Bcl-2 expression, with the downregulation of BAX and caspase-3 expression. C. ignea treatment alleviated all alterations in these parameters, which was further confirmed by the histopathological analysis of lung tissues. The findings of the current work provide the first verification of the preventive and therapeutic potentials of C. ignea extract against benzo(a)pyrene-induced lung tumorigenesis in mice. � 2019 The Authors
Description
Scopus
Keywords
October University for Modern Sciences and Arts, University for Modern Sciences and Arts, MSA University, جامعة أكتوبر للعلوم الحديثة والآداب, Benzo(a)pyrene, Cuphea ignea, Lung tumorigenesis, Plant phenolics, adenosine deaminase, antineoplastic agent, benzo[a]pyrene, beta actin, caspase 3, Cuphea ignea extract, cyclooxygenase 2, immunoglobulin enhancer binding protein, lactate dehydrogenase, macrophage elastase, plant extract, protein Bax, protein bcl 2, tumor marker, unclassified drug, unspecific monooxygenase, vasculotropin, A-549 cell line, aerial plant part, albino mouse, animal cell, animal experiment, animal model, animal tissue, antineoplastic activity, Article, body weight, cancer incidence, carbon nuclear magnetic resonance, carcinogenesis, cell culture, cell viability assay, chemoprophylaxis, column chromatography, controlled study, cytotoxicity, DNA fragmentation, drug induced disease, electrospray mass spectrometry, gene expression, heteronuclear multiple bond correlation, heteronuclear single quantum coherence, histopathology, IC50, in vitro study, in vivo study, lipid peroxidation, liquid chromatography-mass spectrometry, lung cancer, lung parenchyma, lung weight, male, mouse, MTT assay, nonhuman, oxidative stress, paper chromatography, priority journal, proton nuclear magnetic resonance, real time polymerase chain reaction, smoking, thin layer chromatography