Browsing by Author "Aborehab N.M."

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Chemometric discrimination of three Pistacia species via their metabolic profiling and their possible in vitro effects on memory functions
(CIC Edizioni Internazionali s.r.l., 2020) El Bishbishy M.H.; Gad H.A.; Aborehab N.M.; Pharmacognosy Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Pharmacognosy Department; Faculty of Pharmacy; Ain Shams University; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt
Alzheimer's disease (AD) is the most widespread neurodegenerative disease; there are around ten million new cases of Alzheimer yearly worldwide especially in middle or low-income countries. Pistacia is a genus of flowering plants including the well-known, economically important P. chinensis Bunge, P. lentiscus L. and P. khinjuk. In this study, the metabolic profiling of Pistacia leaves extracts was achieved via UHPLC-ESI-MS analysis and GC�MS analysis employing chemometric analysis for their discrimination. In addition, the methanolic extracts of different Pistacia species were assessed for their anti-cholinesterase and anti-inflammatory activities by various in vitro assays. 37 and 30 metabolites belonging to different classes were identified by UHPLC-ESI-MS and GC�MS analyses respectively. Chemometric analysis revealed that P. lentiscus and P. khinjuk were more closely related chemically to each other. All studied Pistacia leaves extracts showed apparent anti-cholinesterase and anti-inflammatory activities, which promotes their use in the prevention and management of AD. � 2019 Elsevier B.V.
Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells
(BioMed Central Ltd., 2019) Aborehab N.M.; Osama N.; Department of Biochemistry; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; 12611; Egypt; Department of Biochemistry; Faculty of Pharmacy; Menoufia University; Menoufia; 32511; Egypt
Background: Cervical cancer is the fourth most common cancer affecting women worldwide. Paclitaxel/Carboplatin is one of the most commonly prescribed regimens in cervical cancer treatment. Although chemotherapeutic drugs are very effective, severe side effects and development of drug resistance limits the use of these drugs. The use of natural products with anticancer activity may help to partially overcome these issues. Methods: In the present study, we investigated the ability of Gallic acid, to potentiate the anti-cancer effects of Paclitaxel, Carboplatin and Paclitaxel/Carboplatin combination in human HeLa cells by performing MTT assay, cell cycle analysis and RT-PCR assay and Western blotting for some apoptotic markers. Results: Our results revealed that the highest cytotoxic effect, the highest induction of apoptosis and significant elevation in P53 and Caspase 3 levels was seen in Paclitaxel/Gallic acid combination. Conclusion: These results indicate that Gallic acid potentiates Paclitaxel effect and that Paclitaxel/Gallic acid combination could represent a promising alternative with lower side effects-for Paclitaxel/Carboplatin combination in treatment of cervical cancer treatment. � 2019 The Author(s).
IL-10 and TGF-?: Roles in chondroprotective effects of Glucosamine in experimental Osteoarthritis?
(Elsevier B.V., 2017) Waly N.E.; Refaiy A.; Aborehab N.M.; Department of Physiology; Faculty of Medicine; Helwan University; Cairo; 11795; Egypt; Department of Pathology Faculty of Medicine; Assiut University; Assiut; 71515; Egypt; Department of Biochemistry; Faculty of Pharmacy; MSA University; Giza; 11787; Egypt
Objective Osteoarthritis (OA) is a complex disease of the whole joint. Glucosamine (GlcN) treatment may have a chondroprotective effect on OA. We investigated the mechanism of action of glucosamine treatment through interleukin-10 (Il-10) and transforming growth factor ?-1 (TGF ?-1). Methods Thirty male albino rats were used. A single intraarticular (i.a.) injection of 2�mg of Monosodium Iodoacetate (MIA) was injected into the knee joint of anesthetized rats. GlcN (50 or 100�mg/kg/day, p.o. for 2 month) was administered orally. Serum levels of Il-10 and TGF-?1 were determined by ELISA. Histopathological changes in treated and control joints were examined using hematoxylin-eosin (H & E) staining. Results The mean serum level of IL-10 significantly decreased in the OA group compared to control group (P value�<�0.0001). On the other hand, mean serum level of IL-10 significantly increased in GlcN treated groups when compared to the OA group (P value�<�0.0001). Serum level of TGF ?-1 was significantly elevated in OA group compared to control group (P value�<�0.0001). On the other hand, the mean serum level of TGF ?-1 was significantly decreased in the GlcN treated groups when compared to the OA group (P value�<�0.0001). Histopathological evaluation of GlcN treated groups showed different grades of healing, according to Osteoarthritis Research Society International (OARSI) grading system. Conclusion Our results showed that IL-10 and TGF-?1 possibly mediate GlcN chondroprotective effects in OA. Both serum biomarkers can be useful in the follow-up of articular cartilage damage in clinical settings. � 2017 Elsevier B.V.
IL-6 and NFE2L2: A putative role for the hepatoprotective effect of N. Sativa, P. Ginseng and C. Sempervirens in AFB-1 induced hepatocellular carcinoma in rats
(Elsevier Inc., 2019) Aborehab N.M.; Waly N.E.; Department of Biochemistry; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University)6th October Giza 12611; Egypt; Department of Physiology; Faculty of Medicine; Helwan University; Cairo; 11795; Egypt; Department of Medical Education; Creighton School of Medicine; Omaha; NE zip 68175; United States
In this study, we investigated possible hepato-protective effects of N. Sativa, P. Ginseng, and C. Sempervirens in Aflatoxin B1 (AFB-1) induced hepatocellular carcinoma rat model. Fifty-four male albino rats were randomly assigned to experimental groups. Alcoholic extracts of aforementioned herbs were administered orally for 28 days at different doses. IL-6, hs-CRP, MDA, SOD and NFE2L2 were determined using ELISA. Histopathological changes in treated groups were examined. Herbal treatment significantly reduced IL-6, hs-CRP, and MDA (P < 0.001) whereas it significantly increased SOD (p < 0.001). C. Sempervirens 600 and N. Sativa 1000 increased NFE2L2 level compared to P. Ginseng 500 group (P value<0.01). Histopathological evaluation of treated groups showed different grades of healing of the liver. This study confirms a beneficial hepatoprotective effect for aforementioned herbal extracts orally administered in rat model of AFB1 induced HCC. This effect is putatively mediated via modulation of inflammatory cytokines as well as amelioration of oxidative stress. � 2019
A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis
(BioMed Central Ltd., 2017) Aborehab N.M.; El Bishbishy M.H.; Refaiy A.; Waly N.E.; MSA University; Department of Biochemistry; Faculty of Pharmacy; Giza; 11787; Egypt; MSA University; Department of Pharmacognosy; Faculty of Pharmacy; Giza; 11787; Egypt; Assiut University; Department of Pathology Faculty of Medicine; Assiut; 71515; Egypt; Department of Physiology; Faculty of Medicine; Helwan University; Cairo; 11795; Egypt
Background: Herbal treatment may have a chondroprotective and therapeutic effect on Osteoarthritis (OA). We investigated the mechanism of action of ginger and curcumin rhizomes cultivated in Egypt in treatment of OA in rat model. Methods: Thirty-five albino rats were intra-articularly injected with Monosodium Iodoacetate in the knee joint. Ginger and curcumin was orally administered at doses of 200 and 400mg/kg (F200 and F400). Serum levels of cartilage oligomeric matrix protein (COMP), hyaluronic acid (HA), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin-1 beta (IL-1?) and superoxide dismutase activity (SOD) were measured using ELISA. The composition of the herbal formula hydro-ethanolic extract was characterized using UPLC-ESI-MS. Histopathological changes in injected joints was examined using routine histopathology. Statistical analysis was performed using one-way ANOVA. Results: Serum levels of COMP, HA, MPO, MDA, and IL-1? were significantly decreased in F 200, F 400 and V groups when compared to OA group (P value <0.0001). On the other hand SOD levels were significantly elevated in treated groups compared to OA groups (P value <0.0001). Conclusions: The ginger/curcumin at 1:1 had chondroprotective effect via anti-inflammatory and antioxidant effect in rat OA model. Further pharmacological and clinical studies are needed to evaluate this effect. � 2017 The Author(s).
Resistin mediates tomato and broccoli extract effects on glucose homeostasis in high fat diet-induced obesity in rats
(BioMed Central Ltd., 2016) Aborehab N.M.; El Bishbishy M.H.; Waly N.E.; Faculty of Pharmacy; MSA University; Department of Biochemistry; Giza; 11787; Egypt; Faculty of Pharmacy; MSA University; Department of Pharmacognosy; Giza; 11787; Egypt; Faculty of Medicine; Helwan University; Department of Physiology; Helwan; 11787; Egypt
Background: Resistin is an adipocyte hormone that regulates glucose metabolism. Elevated levels of resistin may cause insulin resistance. This may link obesity, and increased fat mass to type II diabetes and insulin resistance. We hypothesized that treatment with tomato and broccoli extracts regulates glucose homeostasis via modulation of resistin levels in high fat diet-induced obesity rats (HFD). Methods: Forty-eight male albino rats were divided into 8 groups as follows: control, HFD, stop fat diet (SD), Tomato 200 mg/kg (T200), Tomato 400 mg/kg (T400), Broccoli 200 mg/kg (B200), Broccoli 400 mg/kg (B400), and Chromax (CX). Treatment continued for 1 month. Serum levels of resistin, leptin, adiponectin, glucose and insulin were measured using ELISA and spectrophotometry. Results: Serum levels of resistin were significantly reduced in the T 200, T 400, B 200, B 400 and CX groups to: 4.13 � 0.22 ng/ml, 1.51 � 0.04 ng/ml, 4.13 � 0.22 ng/ml, 2.32 � 0.15 ng/ml and 1.37 � 0.03 ng/ml, respectively, compared to HFD group and SD group (P value < 0.0001). Non-significant differences were found between T 400, B 400 and CX groups. Serum levels of leptin were significantly reduced in the T 400 (22.7 � 0.84 pg/ml) group compared to the B 400 (41 � 2.45 Pg/ml) and CX groups (45.7 � 2.91 Pg/ml), P value < 0.001. Serum levels of adiponectin were significantly increased in the T 400 group (131 � 3.84 pg/ml) compared to the CX group (112 � 4.77 pg/ml), P value < 0.01. Conclusions: Our results demonstrate that tomato and broccoli extract treatment regulates glucose homeostasis via reduction of serum resistin and may be a useful non-pharmacological therapy for obesity. � 2016 The Author(s).
Rho-Kinase II Inhibitory Potential of Eurycoma longifolia New Isolate for the Management of Erectile Dysfunction
(Hindawi Limited, 2019) Ezzat, Shahira M; Okba M.M.; Ezzat M.I.; Aborehab N.M.; Mohamed S.O.; Pharmacognosy Department; Faculty of Pharmacy; Cairo University; Kasr El-Ainy Street; Cairo; 11562; Egypt; Pharmacognosy Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); October; 12566; Egypt; Biochemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th October12566; Egypt; School of Pharmaceutical Sciences; Universiti Sains Malaysia; Malaysia
Background. Eurycoma longifolia Jack (Fam.: Simaroubaceae), known as Tongkat Ali (TA), has been known as a symbol of virility and sexual power. The aim of the study was to screen E. longifolia aqueous extract (AE) and isolates for ROCK-II inhibition. Results. The AE (1-10 ?g/ml) showed a significant inhibition for ROCK-II activity (62.8-81%) at P < 0.001 with an IC50 (651.1 � 32.9 ng/ml) compared to Y-27632 ([(+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride]) (68.15-89.9 %) at same concentrations with an IC50 (192 � 8.37 ng/ml). Chromatographic purification of the aqueous extract (AE) allowed the isolation of eight compounds; stigmasterol T1, trans-coniferyl aldehyde T2, scopoletin T3, eurycomalactone T4, 6?- hydroxyeurycomalactone T5, eurycomanone T6, eurycomanol T7, and eurycomanol-2-O-?-D-glucopyranoside T8. This is the first report for the isolation of T1 and T3 from E. longifolia and for the isolation of T2 from genus Eurycoma. The isolates (at 10 ?g/ml) exhibited maximum inhibition % of ROCK-II 82.1 � 0.63 (T2), 78.3 � 0.38 (T6), 77.1 � 0.11 (T3), 76.2 � 3.53 (T4), 74.5 � 1.27 (T5), 74.1 � 2.97 (T7), 71.4 � 2.54 (T8), and 60.3 � 0.14 (T1), where the newly isolated compound trans-coniferyl aldehyde T2 showed the highest inhibitory activity among the tested isolated compounds and even higher than the total extract AE. The standard Y-27632 (10 ?g/ml) showed 89.9 � 0.42 % inhibition for ROCK-II activity when compared to control at P < 0.0001. Conclusion. The traditional use of E. longifolia as aphrodisiac and for male sexual disorders might be in part due to the ROCK-II inhibitory potential. � 2019 Shahira M. Ezzat et al.