Browsing by Author "Abdelmohsen U.R."

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Anti-Alzheimer potential, metabolomic profiling and molecular docking of green synthesized silver nanoparticles of Lampranthus coccineus and Malephora lutea aqueous extracts
(Public Library of Science, 2019) Youssif K.A.; Haggag E.G.; Elshamy A.M.; Rabeh M.A.; Gabr N.M.; Seleem A.; Alaraby Salem M.; Hussein A.S.; Krischke M.; Mueller M.J.; Abdelmohsen U.R.; Department of Pharmacognosy; Faculty of Pharmacy; Modern University for Technology and Information; Cairo; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Helwan University; Cairo; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Department of Pharmacology; National Research Centre; Cairo; Egypt; Department of Pharmaceutical Chemistry; October University for Modern Sciences and Arts (MSA); Cairo; Egypt; Julius-von-Sachs-Institute of Biosciences; Biocenter; Pharmaceutical Biology; University of W�rzburg; W�rzburg; Germany; Department of Pharmacognosy; Faculty of Pharmacy; Minia University; Minia; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Deraya University; Universities Zone; New Minia City; Minia; Egypt
The green synthesis of silver nanoparticles (SNPs) using plant extracts is an eco-friendly method. It is a single step and offers several advantages such as time reducing, cost-effective and environmental non-toxic. Silver nanoparticles are a type of Noble metal nanoparticles and it has tremendous applications in the field of diagnostics, therapeutics, antimicrobial activity, anticancer and neurodegenerative diseases. In the present work, the aqueous extracts of aerial parts of Lampranthus coccineus and Malephora lutea F. Aizoaceae were successfully used for the synthesis of silver nanoparticles. The formation of silver nanoparticles was early detected by a color change from pale yellow to reddish-brown color and was further confirmed by transmission electron microscope (TEM), UV�visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), and energy-dispersive X-ray diffraction (EDX). The TEM analysis of showed spherical nanoparticles with a mean size between 12.86 nm and 28.19 nm and the UV- visible spectroscopy showed ?max of 417 nm, which confirms the presence of nanoparticles. The neuroprotective potential of SNPs was evaluated by assessing the antioxidant and cholinesterase inhibitory activity. Metabolomic profiling was performed on methanolic extracts of L. coccineus and M. lutea and resulted in the identification of 12 compounds, then docking was performed to investigate the possible interaction between the identified compounds and human acetylcholinesterase, butyrylcholinesterase, and glutathione transferase receptor, which are associated with the progress of Alzheimer�s disease. Overall our SNPs highlighted its promising potential in terms of anticholinesterase and antioxidant activity as plant-based anti-Alzheimer drug and against oxidative stress. � 2019 Youssif et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Antiviral potential of green synthesized silver nanoparticles of lampranthus coccineus and malephora lutea
(Dove Medical Press Ltd., 2019) Haggag E.G.; Elshamy A.M.; Rabeh M.A.; Gabr N.M.; Salem M.; Youssif K.A.; Samir A.; Bin Muhsinah A.; Alsayari A.; Abdelmohsen U.R.; Department of Pharmacognosy; Faculty of Pharmacy; Helwan University; Cairo; 11795; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Modern University for Technology and Information; Cairo; Egypt; Department of Pharmaceutical Chemistry; October University for Modern Sciences and Arts (Msa); Cairo; Egypt; Department of Pharmacognosy; College of Pharmacy; King Khalid University; Abha; 61441; Saudi Arabia; Department of Pharmacognosy; Faculty of Pharmacy; Minia University; Minia; Egypt
Background: Viral and microbial infections constitute one of the most important life-threatening problems. The emergence of new viral and bacterial infectious diseases increases the demand for new therapeutic drugs. Purpose: The objective of this study was to use the aqueous and hexane extracts of Lampranthus coccineus and Malephora lutea F. Aizoaceae for the synthesis of silver nanoparticles, and to investigate its possible antiviral activity. In addition to the investigation of the phytochemical composition of the crude methanolic extracts of the two plants through UPLC-MS metabolomic profiling, and it was followed by molecular docking in order to explore the chemical compounds that might contribute to the antiviral potential. Methods: The formation of SNPs was further confirmed using a transmission electron microscope (TEM), UV-Visible spectroscopy and Fourier transform infrared spectroscopy. The antiviral activity of the synthesized nanoparticles was evaluated using MTT assay against HSV-1, HAV-10 virus and Coxsackie B4 virus. Metabolomics profiling was performed using UPLC-MS and molecular docking was performed via Autodock4 and visualization was done using the Discovery studio. Results: The early signs of SNPs synthesis were detected by a color change from yellow to reddish brown color. The TEM analysis of SNPs showed spherical nanoparticles with mean size ranges between 10.12 nm to 27.89 nm, and 8.91 nm 14.48 nm for Lampranthus coccineus and Malephora lutea aqueous and hexane extracts respectively. The UV-Visible spectrophotometric analysis showed an absorption peak at ?max of 417 nm.The green synthesized SNPs of L. coccineus and M. lutea showed remarkable antiviral activity against HSV-1, HAV-10, and CoxB4 virus. Metabolomics profiling of the methanolic extract of L. coccineus and M. lutea resulted in identifying 12 compounds. The docking study predicted the patterns of interactions between the compounds of L. coccineus and M. lutea with herpes simplex thymidine kinase, hepatitis A 3c proteinase, and Coxsackievirus B4 3c protease, which was similar to those of the co-crystal inhibitors and this can provide a supposed explanation for the antiviral activity of the aqueous and nano extracts of L. coccineus and M. lutea. Conclusion: These results highlight that SNPs of L. coccineus and M. lutea could have antiviral activity against HSV-1, HAV-10, and CoxB4 virus. � 2019 Haggag et al. This work is published and licensed by Dove Medical Press Limited.
Metabolomic profiling and biological investigation of Tabebuia Aurea (Silva Manso) leaves, family Bignoniaceae
(Taylor and Francis Ltd., 2019) Mahmoud B.K.; Hamed A.N.E.; Samy M.N.; Abdelmohsen U.R.; Attia E.Z.; Fawzy M.A.; Refaey R.H.; Salem M.A.; Pimentel-Elardo S.M.; Nodwell J.R.; Desoukey S.Y.; Kamel M.S.; Department of Pharmacognosy; Faculty of Pharmacy; Minia University; Minia; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Deraya University; New Minia City; Egypt; Departmentof Biochemistry; Faculty of Pharmacy; Minia University; Minia; Egypt; Department of Pharmaceutical Chemistry; Faculty of Pharmacy; October University of Modern Sciences and Arts (MSA); Giza; Egypt; Department of Biochemistry; University of Toronto; Toronto; ON; Canada
Both ethyl acetate and aqueous fractions of Tabebuia aurea leaves exhibited noteworthy antioxidant and nephroprotective activities against carbon tetrachloride (CCl4)-induced nephrotoxicity in rats, as evidenced by the remarkable improvements of renal serum biomarkers and histopathological features. Additionally, the ethyl acetate fraction displayed a prominent in vitro antitrypanosomal activity against Trypanosoma brucei; consequently, the leaves were subjected to LC-HR-ESI-MS metabolomic profiling to discover the constituents that possibly underlie their bioactivities. Therefore, ten metabolites were characterized, mostly dominated by flavonoids. Interestingly, two identified constituents viz., 3,9,12,15-octadecatetraenoic acid (9) and 9,11,13-octadecatrienoic acid (10) are reported firstly herein from the genus Tabebuia. Furthermore, among the dereplicated constituents, rutin (5) and kaempferol 3-O-rutinoside (6) exhibited the highest docking scores as effective antitrypanosomal compounds. � 2019, � 2019 Informa UK Limited, trading as Taylor & Francis Group.