Browsing by Author "Abdel Motaal, Amira"

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Antihyperglycemic Activity and Standardization of the Bioactive Extract of Cleome droserifoliaGrowing in Egypt
(Pharmacognosy Journal, 2014) Abdel Motaal, Amira; Ezzat, Shahira M; El-Askary, Hesham
Cleome droserifolia herb is well known in the Egyptian folk medicine for the treatment of diabetes. However, a standardized active extract of the herb was never prepared for incorporation into a pharmaceutical dosage form.
In vitro and in vivo antidiabetic potential of extracts and a furostanol saponin from Balanites aegyptiaca
(Taylor & Francis, 2017) Ezzat, Shahira M; Abdel Motaal, Amira; Abdel Wanees El Awdan, Sally
Context:Balanites aegyptiacaDel. (Zygophyllaceae) fruits are well-known antidiabetic drug in Egyptian folk medicine. Nevertheless, its mechanism of action is still unclear. Objectives:Searching for the possible mechanisms of action of the plant and identification of its bio- active compounds. Materials and methods:A bio-guided protocol based on the evaluation ofa-glucosidase (AG) and aldose reductase (AR) inhibitory activities was adopted to isolate the biologically active compounds from the methanol extract (MeEx). Anin vivoantidiabetic study was conducted for the active extract, fraction and compound using streptozotocin-induced diabetic male albino Wistar rats at two dose levels (100 and 200 mg/kg.b/wt) for 2 weeks. Results:Three compounds were isolated and identified: a sterol, (1) stigmasterol-3-O-b- D-glucopyranoside; a pregnane glucoside, (2) pregn-5-ene-3b,16b,20(R)-trio1-3-O-b- D-glucopyranoside; a furostanol saponin, (3)26-(O-b- D-glucopyranosyl)-22-O-methylfurost-5-ene-3b,26-diol-3-O-b-D-glucopyranosyl-(1!4)-[a-L-rham- nopyranosyl-(1!2)]-b- D-glucopyranoside. Only compound3possessed significant AG and AR inhibitory activities (IC 50 ¼3.12 ± 0.17 and 1.04 ± 0.02lg/mL, respectively), while compounds1and2were inactive. Thein vivoantidiabetic study revealed that MeEx and furostanol saponin3possessed significant activities at a dose of 200mg/kg through reducing the fasting plasma glucose level by 46.14% and 51.39%, respect- ively, as well as reducing the total cholesterol by 24.44% and 31.90%, respectively. Compound3also caused increment in insulin and C-peptide levels by 63.56% and 65%, respectively. Discussion and conclusions:We presented a scientific base for usingBalanites aegyptiaca, and shed the light on one of its saponins, as an antidiabetic agent in fasting and postprandial hyperglycaemia along with the improvement of diabetic complications.
In vivo anti-inflammatory activity of caffeoylquinic acid derivatives from solimano virgaurea in rats
(Taylor & Francis, 2016) Abdel Motaal, Amira; Ezzat, Shahira M; G Tadros, Mariane; El-Askary, Hesham I
Solidago virgaurea L. (Asteraceae) is traditionally used as an anti-inflammatory for the treatment of various symptoms including cystitis. However, little is known concerning the constituents responsible for this activity and the mechanism of their action.
Isolation of New Cytotoxic Metabolites from Cleome droserifolia Growing in Egypt
(Zeitschrift für Naturforschung, 2012) Ezzat, Shahira M; Abdel Motaal, Amira
The sulforhodamine B (SRB) assay was used to assess the cytotoxicity of the aqueous (AqEx) and ethanolic (AlEx) extracts, respectively, of the aerial parts of Cleome droserifolia (Forssk.) Del. against two human cancer cell lines, breast (MCF7) and colon (HCT116) adenocarcinoma. AqEx exhibited higher cytotoxic activity, thus its four subfractions, namely n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuFr) fractions, were also tested. Purifi cation of the more active ClFr and EtFr yielded nine compounds. Six terpenoids, guai-7(11),8-diene (C1), 1-hydroxy-guai-3,10(14)-diene (C2), 18-hydroxydollabela- 8(17)-ene (C3), (24E)-stigmasta-5,8-dien-3β-ol (C4), teucladiol [1α,5β-guai-10(14)- ene-4β,6β-diol] (C5), and buchariol (4,10-epoxy-6α-hydroxyguaiane) (C6), were isolated from ClFr and three fl avonol glycosides, isorhamnetin-3-O-β-D-glucoside (F1), quercetin- 3`-methoxy-3-O-(4``-acetylrhamnoside)-7-O-α-rhamnoside (F2), and kaempferol-4`-methoxy- 3,7-O-dirhamnoside (F3), were isolated from EtFr. Compounds C3 and F2 are new in nature. The isolated compounds were identifi ed using various spectroscopic methods (UV, IR, 1H NMR,13C NMR, HMQC, HMBC, and COSY). Compounds C1, C3, F2, and F3 showed signifi cant cytotoxic activities against the two tested cell lines comparable to those of the anticancer drug doxorubicin®. The new compound C3 was the most active as it had the lowest IC50 values, (1.9  0.08) and (1.6  0.09) μg/ml corresponding to 6.5 and 5.4 μM, against MCF7 and HCT116 cells, respectively.
Protective effects of beetroot extract against phenyl hydrazine induced anemia in rats
(Phcog J, 2014) Jaiswal, Anupam; Ganeshpurkar, Aditya; Awasthi, Ankita; Bansal, Divya; Dubey, Nazneen; Kumar Singh, Amrit; Deep, Prakash; Dubey, Suchita; Paul Attrey, Dharam; Naved, Tanveer; Kamil, Noor; Syed Imran-ul-Haque, Hafiz; Abdel Motaal, Amira; Ezzat, Shahira M; El-Askary, Hesham; Vladimirovna Daironas, Janna; Kazbekovna Serebryanaya, Fatima; Nazimovich Zilfikarov, Ifrat; Silvia, Netala; Rajeswari, CH; Mounica, D; Manasa, R; Prasanth, DSNBK; Bhandarkar, Anant V; Shashidhara, S; Deepak, M; Thube, SA; Patil, MJ
Introduction: Brassica juncea is an economically important plant that has been well-known in India for centuries for its medicinal and nutritive values. The broad spectrum of benefi cial effects of the seeds perceived with this plant warrants further exploration of B. juncea seeds as a potential source for obtaining pharmacologically standardized phytotherapeutics, which could be potentially useful. The objective of the present study was to perform the pharmacognosy of mustards seeds inclusive of qualitative and quantitative phytochemical analysis, fi ngerprinting by infrared spectroscopy and high performance thin layer chromatography analysis and toxicity assessment in vitro. Methods: Different sections of seeds were taken and stained with 0.1% phloroglucinol for microscopic examination. The seeds were extracted by 80% alcohol on a rotary shaker to perform phytochemical analysis and fi ngerprinting. The toxicity assessment of this extract was performed on human dermal fi broblast cells. Results: Microscopic examination of seeds showed characteristic features of mustard seeds. The extraction of these seeds by 80% alcohol resulted in IC50 value of 103 ± 3 μg/mL for 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl radical scavenging assay. The fi ngerprinting analysis of this extract indicated probable presence of sinigrin, quercetin, vanillin, catechin, vitamin E and sulfur-containing compounds. This extract exhibited 50% toxicity (IC50) at 1.79 mg/mL. Conclusion: The result achieved will be used to assess the therapeutic effi cacy of seed extracts for future pharmacological evaluations.
Randomized double-blinded pilot clinical study of the antidiabetic activity of Balanites aegyptiaca and UPLC-ESI-MS/MS identification of its metabolites
(Taylor & Francis, 2017) Rashad, Hend; M Metwally, Fateheya; Ezzat, Shahira M; M Salama, Maha; Hasheesh, Adel; Abdel Motaal, Amira
Balanites aegyptiaca Del. (Zygophyllaceae) fruits are traditionally known for the treatment of hyperglycaemia. Several in vitro and in vivo studies proposed some mechanisms of action. However, clinical trials in human beings were never reported to date.
Upregulation of MC4R and PPAR-α expression mediates the anti-obesity activity of Moringa oleifera Lam. in high-fat diet-induced obesity in rats
(Elsevier Ireland Ltd, 01/01/2020) Ezzat, Shahira M; El Bishbishy, Mahitab H.; Aborehab, Nora M; Salama, Maha M.; Hasheesh, Adel; Abdel Motaal, Amira; Rashad, Hend; Metwally, Fateheya M
Ethnopharmacological relevance: various extracts of Moringa oleifera Lam. leaves, were reported to possess antiobesity effect in experimental animals models, yet its active doses and mechanism of action are still unclear. Materials and methods: The metabolic profiling of 70% ethanol extract of M. oleifera (MO) leaves was performed using HPLC-MS/MS analysis. The antiobesity activity of MO was tested in high fat diet induced obesity in rats at 200 and 400 mg/kg body weight orally for 1 month. Total cholesterol (TC), high density lipoproteins (HDL-C), low density lipoprotein-cholesterol (LDL-C), triglycerides (TGs), insulin resistance, insulin sensitivity, and adipose tissue index were monitored. In addition, fatty acid synthase (FAS) and HMG-CoA reductase mRNA from liver tissue, Peroxisome Proliferator-Activated Receptor alpha (PPARα) and Melanocortin-4 receptor (MC4R) RNA from adipose tissue were quantified using qRT-PCR. MO hard gelatin capsules (400 mg/capsule) were formulated and standardized using HPLC-RP analysis and tested on fifteen female participants, aged 45–55 with a BMI of 29–34 kg/m2. Results: Thirteen metabolites were tentatively identified using HPLC-MS/MS analysis including flavonols, flavones and a phenolic acid. MO 400 showed a prominent effect on reducing the rats’ final weights, % weight increase and adiposity index (P < 0.05). Glucose, insulin and HOMA-IR were significantly reduced and R-QUICKI was significantly increased by MO 400 (P < 0.001). Mean tissue level of leptin and vaspin were significantly reduced, adiponectin, omentin and GLUT-4 expression were increased significantly by MO 400 (P < 0.01). MO 400 significantly suppressed FAS and HMG-CoA reductase and increased mRNA expression of MC4R and PPAR-α (P < 0.01). Eight weeks administration of MO hard gelatin capsules to obese patients showed significant reduction of the average BMI, TC and LDL compared to the baseline values (p < 0.05). Conclusion: Our results presented a scientific evidence for the traditional use of M. oleifera leaves as antiobesity herbal medicine