Browsing by Author "A. Kandil, Zeinab"

Now showing 1 - 3 of 3

Antibacterial, antioxidant, and topical antiinflammatory activities of Bergia ammannioides: A wound-healing plant
(Informa Healthcare, 2015) Ezzat, Shahira M; A. Choucry, Mouchira; A. Kandil, Zeinab
Context: Despite the traditional use of Bergia ammannioides Henye ex Roth. (Elatinaceae) for the treatment of wounds in India, there is a scarcity of scientific data supporting this use. Objective: The objective of this study is to assess wound-healing potentiality of the plant, to study pharmacological activities that may contribute in eliminating wound complications, and to investigate the biologically active fractions. Material and methods: The ethanolic extract (EtOH) of the aerial parts was fractionated to obtain n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuOH) fractions. EtOH and its fractions were formulated in strength of 5 and 10% w/w ointment and tested for wound-healing activity using the excision model. The topical anti-inflammatory, in vitro antioxidant, and antibacterial activities were evaluated. HxFr and EtFr were chemically investigated to isolate their constituents. Results: Application of EtOH, HxFr, and EtFr (10% w/w ointments) leads to 71.77, 85.62, and 81.29% healing of the wounds with an increase in the collagen content. HxFr had the strongest anti-inflammatory (64.5% potency relative to Voltaren) and antibacterial activity (MIC ¼ 104 mg/ml against Staphylococcus aureus), while EtFr showed the strongest antioxidant activity against DPPH, ABTS+ , and super oxide radical with an IC50 value of 10.25 ± 0.01, 66.09 ± 0.76, and 167.33 ± 0.91 mg/ml, respectively. b-Sitosterol, lupeol, cyclolaudenol, and cycloartenol were isolated from HxFr. Quercetin, ellagic acid, kaempferol-3-O-a-L-rhamnoside, and quercetin-3-O-a-L-rhamnoside were isolated from EtFr. Discussion and conclusion: Our study presents scientific evidence for the efficacy of B. ammannioides in enhancing wound healing, and the first isolation of cyclolaudenol and cycloartenol from Bergia
BIOLOGICAL ACTIVITY AND STANDARDIZATION OF THE ETHANOLIC EXTRACT OF THE AERIAL PARTS OF MENTHA SUAVEOLENS EHRH
(World Journal of Pharmacy and Pharmaceutical Sciences, 2014) Ezzat, Shahira M; I. El-Askary, Hesham; A. El-Kashoury, El-Sayeda; A. Kandil, Zeinab; A. Salem, Mohamed
A biologically guided fractionation of the ethanolic extract of the aerial parts of Mentha suaveolens Ehrh. cultivated in Egypt revealed that the ethyl acetate fraction had the highest hepatoprotective activity as it prevented the increase caused by CCl4 in the levels of aspartate aminotransferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) enzymes by 51.6%, 57.0% and 56.7%, respectively. The same fraction also showed the highest antioxidant activity; in vivo (as it restored the glutathione level in diabetic rats by 98.0 %) and in vitro as it had the highest free radical scavenging activity (IC50=31g/ mL). It also had the highest cytotoxic activity against human liver carcinoma cell line (HEPG2) (IC50 = 5.1 μg/ mL). Consequently, this fraction was purified to yield caffeic acid and rosamarinic acid. Rosmarinic acid, the major component possessed cytotoxic activity on HEPG2 (IC50 = 4.5 μg/ mL) comparable to doxorubicin (IC50 = 3.73 μg/mL). A rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the standardization of the active ethyl acetate fraction and the method was validated. Rosmarinic acid content in M. suaveolens was estimated by RPHPLC, to be 1.16 and 50.17% w/w for the powdered aerial parts and ethyl acetate fraction, respectively.
Chemical and biological study of Mentha suaveolens Ehrh. cultivated in Egypt
(Journal of Medicinal Plant Research, 2014) Ezzat, Shahira M; A. El-Kashoury, El-Sayeda; A. Kandil, Zeinab; A. Salem, Mohamed; A. Sleem, Amany
Investigation of the different fractions of the ethanolic extract of the aerial parts of M. suaveolens growing in Egypt yielded nine compounds; two new triterpenes [3β-acetyl -22α-hydroxy ursa-12,20- diene (compound 1) and 2α, 3β-dihydroxy-olean-18-en-29-oic acid (compound 7)] and nine known compounds: a sterol and its glucoside; β-sitosterol (compound 2) and β-sitosterol-3-O- β-D-glucoside (compound 4), a triterpene; oleanolic acid (compound 3), two monoterepenes; dihydrolimonene (compound 5) and 7-hydroxy-p-cymene (compound 6), two flavonoids; isoquercitrin (compound 8) and rutin (compound 9) which were isolated from M. suaveolens Ehrh. for the first time. The structures of the isolated compounds were identified by spectral data (UV, MS, 1D and 2D-NMR) and comparison with authentic samples. Moreover, the ethanolic extract showed potent analgesic activity as compared to indomethacin. The ethyl acetate fraction was the most potent as anti-inflammatory (88%), followed by the ethanolic extract (82.9%) as compared with indomethacin. The ethanolic extract and its four subfractions showed a moderate inhibitory activity against the tested human pathogenic bacteria.