Browsing by Author "A. Al-Gendy, Amal"

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Production of Flavonoids and Phenolic Compounds by Elicitation of Iphiona mucronata (Forssk.) Asch. & Schweinf (Asteraceae) Callus and Suspension Cultures
(International Journal of Pharmacognosy and Phytochemistry, 2015) A. Al-Gendy, Amal; O. Bakr, Riham; D. El-Gindi, Omayma
Callus and suspension cultures of Iphiona mucronata grown on Murashige and Skoog medium, supplemented with 0.1 mg/l kinetin (Kn), 0.1 mg/l naphthalene acetic acid (NAA) and 5 mg/l ascorbic acid were elicited with different elicitors. Total flavonoids and phenolics were determined by aluminum chloride - potassium acetate and Folin-Ciocalteu colorimetric methods respectively. When callus and suspension cultures elicited with 10 mg/l yeast extract (Y), it showed an increase of 2.8 and 3.7 folds for flavonoids production respectively. When callus cultures treated with 0.001 mM phenyl alanine (Ph), a slight increase in flavonoids content reached 1.17 folds, while treated suspension reached maximum level after 60 hours. Methyl jasmonate (MJ; 150 µM) increased flavonoids concentration 4 times for callus and 2.3 times for suspension. Determination of phenolics for elicited suspension showed an increase of 2.3 (Y), 2.5 (Ph) and 1.3 (MJ) times. The obtained results favored the use of yeast extract for high productivity and viability of cell lines.
Production of flavonoids in callus cultures of Iphiona mucronata, Astraceae
(Journal of Biomedical Science, 2008) A. Al-Gendy, Amal; D. El-gindi, Omayma; O. Bakr, Reham
Different callus cultures of Iphiona mucronata were established using seedling explants and Murashige & Skoog media (MS) with different phytohormones combinations. The time course for growth of callus culture was followed for the most stable and healthy growing cell lines. Addition of Spirulina platensis extract improved the callus characters but did not increase the growth. The concentration of total flavonoids was calculated by using standard curve for rutin. Total flavonoids produced by callus grown on MS media supplied with 0.1 mg/l kinetin and 0.1 mg/l αnaphthalene acetic acid represents 40% of the plant itself. Addition of 5% Spirolina. platensis extract decreased the productivity of total flavonoids to 21%. Callus grown on MS media supplied with 0.1 mg/l zeatin and 0.1 mg/l α-naphthalene acetic acid produced 24% of total flavonoids compared to the plant itself.
Somatic embryogenesis and plant regeneration from callus and suspension cultures of Iphiona mucronata (Forssk)
(European Scientific Journal, 2013) A. Al-Gendy, Amal; O. Bakr, Riham; D. El-gindi, Omayma
A protocol was designed for plant regeneration of Iphiona mucronata from embryogenic callus via somatic embryogenesis to enable micro propagation of this endangered plant. The embryogenic callus was induced using seedling cultured for nine months on Murashig and Skoog medium (MS) supplemented with 0.1 mg l-1 naphthalene acetic acid (NAA), 0.1 mg l1 kinetin (Kn) and 5 mg l-1 ascorbic acid and incubated in the dark followed by growing on hormone free medium. Transfer of developed embryos to MS medium supplemented with 0.5 mg l-1 kinetin induced shoot formation. Four treatments were further tried for plant development by using indole acetic acid (IAA) or indole butyric acid (IBA) alone or in combination with kinetin. The results showed that 2 mg l-1 IAA was the best for in vitro plantlet regeneration. Embryogenic suspension was induced by transfer of embryogenic callus to liquid medium having the same composition followed by hormone free medium where different stages of embryos were monitored. Shoots were developed upon transfer to liquid medium supplemented with 0.5 mg l-1 Kn. However, no further development appeared upon transfer to semi solid medium containing different phytohormones. Embryogenic callus showed the highest phenolic contents when compared with embryogenic suspension, regenerated plantlets and the parent plant while flavonoids were detected only in embryogenic callus.