Epidermal Growth Factor Restores Cytokeratin Expression in Rats with Diabetes
Loading...
Date
2018-02
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
AMBER PUBLICATION
Series Info
JOURNAL OF RESEARCH IN MEDICAL AND DENTAL SCIENCE;Volume: 6 Issue: 1 Pages: 196-203
Scientific Journal Rankings
Abstract
Diabetes Mellitus (DM) has been associated with salivary glands dysfunction and disorders. Epidermal Growth Factor (EGF) is highly important in the process of wound healing in different parts of the body. The aim of the present study is to investigate the capacity of EGF to reverse the side effects of Streptozotocin (STZ) induced diabetes in the submandibular salivary glands of adult Albino male rats, through immunohistochemical localization of cytokeratin in the parenchyma of the glands. Sixty rats were used in this study and were divided into three equal groups, a control group, an STZ induced group and EGF group where STZ induced rats are treated with a daily EGF injection of a dose of 10 mu g/Kg body weight for two months. The overall expression of cytokeratin in the submandibular salivary glands of STZ induced rats decreased significantly when compared to the control group. However, in EGF group, cytokeratin expression levels were restored to values that were nearly equal to normal levels. Daily intraperitoneal injection of epidermal growth factor in a dose of 10 mu g/Kg body weight for two months, helped reverse the negative side effects of DM in STZ induced rats. However, a small amount of permanent damage was still evident. Future research will be conducted to confirm the above findings through immunohistochemical expression of myosin and E-cadherin as well as ultrastructural examination.
Description
Accession Number: WOS:000428114000032
Keywords
University for University for EGF, TUMOR, CELLS, HEALTH, KERATIN, NEO-EPITOPE, HUMAN-TISSUES, PLEOMORPHIC ADENOMAS, IMMUNOHISTOCHEMICAL DETECTION, SALIVARY-GLANDS, Cytokeratin, Streptozotocin, Diabetes, Epidermal Growth Factor, Submandibular Salivary Gland
Citation
Salivary Defensins and their importance in oral health and disease By: Abiko, Yoshihiro; Saitoh, Masato CURRENT PHARMACEUTICAL DESIGN Volume: 13 Issue: 30 Pages: 3065-3072 Published: 2007 Times Cited: 36 2. Epidermal growth factor and diabetes mellitus; friends or faux? By: Al-Ankily, MM; Shamel, M; Bakr, MM. International Journal of Medical and Dental Sciences. Volume: 5 Issue: 2 Pages: 1290-96 Published: 2016 Times Cited: 1 3. Comparative Cytokeratin Expression in the Different Cell Types of Salivary Gland Mucoepidermoid Carcinoma By: Azevedo, Rebeca Souza; de Almeida, Oslei Paes; Kowalski, Luiz Paulo; et al. HEAD & NECK PATHOLOGY Volume: 2 Issue: 4 Pages: 257-264 Published: DEC 2008 Times Cited: 22 4. Myoepithelial Cells (MEC) of the Salivary Glands in Health and Tumours By: Chitturi, Ravi Teja; Veeravarmal, V.; Nirmal, R. Madhavan; et al. JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH Volume: 9 Issue: 3 Pages: ZE14-ZE18 Published: MAR 2015 Times Cited: 15 5. Keratin expression in human tissues and neoplasms By: Chu, PG; Weiss, LM HISTOPATHOLOGY Volume: 40 Issue: 5 Pages: 403-439 Published: MAY 2002 Times Cited: 531 6. Immunohistochemical expression of CK7, CK5/6, CK19, and p63 in Warthin tumor By: Daguci, Luminita; Stepan, A.; Mercut, Veronica; et al. ROMANIAN JOURNAL OF MORPHOLOGY AND EMBRYOLOGY Volume: 53 Issue: 3 Pages: 603-607 Published: 2012 Times Cited: 7 7. Health benefits of saliva: a review By: Dodds, MWJ; Johnson, DA; Yeh, CK JOURNAL OF DENTISTRY Volume: 33 Issue: 3 Pages: 223-233 Published: MAR 2005 Times Cited: 305 8. IMMUNOHISTOCHEMICAL IDENTIFICATION OF CYTOKERATINS IN THE RAT SUBMANDIBULAR SALIVARY GLANDS DURING ONTOGENESIS By: Dozic, I; Tatjana, Todorovic; Dozic, B.; et al. ACTA VETERINARIA-BEOGRAD Volume: 59 Issue: 1 Pages: 69-80 Published: 2009 Times Cited: 1 9. Submandibular glands as an evident of the effects of antioxidant on alloxan-induced diabetic rats. By: El-Gusbi, G. A. M.; Shredah, M. T. H.; Soliman, A. E. E. World Journal of Medical Sciences Volume: 11 Issue: 2 Pages: 210-216 Published: 2014 Times Cited: 2 10. Cadmium modulates diabetes-induced alterations in murine neuromuscular junction By: Fahim, MA; Hasan, MY; Alshuaib, WB ENDOCRINE RESEARCH Volume: 26 Issue: 2 Pages: 205-217 Published: 2000 Times Cited: 4 11. Title: [not available] By: Fouad, BG; Ghali, LS; Ali, MM. Ultrastructural and immunohitochemical investigation on the effect of certain commonly used diuretucs on the submandibular salivary glands of the rats, with special reference to the incidence of dental caries Published: 2005 PhD Thesis Publisher: Suez Canal University Times Cited: 1 12. Immunohistochemical detection of cytokeratin 18 and its neo-epitope in Warthin's tumor (adenolymphoma) of the parotid glands By: Fukuda, M; Tanaka, A; Kitada, M; et al. ANTICANCER RESEARCH Volume: 21 Issue: 1A Pages: 109-112 Published: JAN-FEB 2001 Times Cited: 3 13. Type 2 diabetes impairs implant osseointegration capacity in rats By: Hasegawa, Hideki; Ozawa, Shogo; Hashimoto, Kazuyoshi; et al. INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS Volume: 23 Issue: 2 Pages: 237-246 Published: MAR-APR 2008 Times Cited: 48 14. IMMUNOREACTION OF KERATIN, ACTIN, S-100 PROTEIN AND RAT-EGF IN DUCTLIGATED RAT SALIVARY-GLANDS By: HASHIMOTO, J; YAMADA, K; OGATA, K; et al. JOURNAL OF ORAL PATHOLOGY & MEDICINE Volume: 21 Issue: 5 Pages: 214-220 Published: MAY 1992 Times Cited: 14 15. Melatonin preserves the morphology of parotid gland damaged by streptozotocin induced diabetes By: Hidayat, M; Tahir, M; Shoro, AA. Journal of Postgraduate Medical Institute (Peshawar-Pakistan). Volume: 28 Issue: 2 Pages: 128-32 Published: 2014 Times Cited: 1 16. Keratins: a structural scaffold with emerging functions By: Kirfel, J; Magin, TM; Reichelt, J CELLULAR AND MOLECULAR LIFE SCIENCES Volume: 60 Issue: 1 Pages: 56-71 Published: JAN 2003 Times Cited: 119 17. Immunohistochemical detection of cytokeratin 18 and its neo-epitope in human salivary glands and pleomorphic adenomas By: Kusama, K; Jiang, Y; Ohno, J; et al. ANTICANCER RESEARCH Volume: 20 Issue: 4 Pages: 2485-2487 Published: JUL-AUG 2000 Times Cited: 12 18. Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rat By: Kwon, Young-Bae; Kim, Hvun-Woo; Roh, Dae-Hyun; et al. JOURNAL OF VETERINARY SCIENCE Volume: 7 Issue: 2 Pages: 105-109 Published: JUN 2006 Times Cited: 43 19. Title: [not available] By: Leutholtz, BC; Ripoll, I. Exercise and disease management Pages: 25 Published: 2011 Publisher: CRC Press, Boca Raton Times Cited: 1 20. Plasmacytoid cells in salivary-gland pleomorphic adenomas: evidence of luminal cell differentiation By: Ogawa, Y; Kishino, M; Atsumi, Y; et al. VIRCHOWS ARCHIV Volume: 443 Issue: 5 Pages: 625-634 Published: NOV 2003 Times Cited: 21 21. Salivary EGF levels reduced in diabetic patients By: Oxford, GE; Tayari, L; Barfoot, MD; et al. JOURNAL OF DIABETES AND ITS COMPLICATIONS Volume: 14 Issue: 3 Pages: 140-145 Published: MAY-JUN 2000 Times Cited: 22 22. MYOEPITHELIUM OF SALIVARY-GLANDS By: REDMAN, RS MICROSCOPY RESEARCH AND TECHNIQUE Volume: 27 Issue: 1 Pages: 25-45 Published: JAN 1 1994 Times Cited: 99 23. IMMUNOHISTOCHEMICAL LOCALIZATION OF KERATIN IN NORMAL HUMAN-TISSUES By: SCHLEGEL, R; BANKSSCHLEGEL, S; PINKUS, GS LABORATORY INVESTIGATION Volume: 42 Issue: 1 Pages: 91-96 Published: 1980 Times Cited: 369 24. The many faces of acinic cell carcinomas of the salivary glands: a study of 40 cases relating histological and immunohistological subtypes to clinical parameters and prognosis By: Schwarz, Stephan; Zenk, Johannes; Mueller, Maximilian; et al. HISTOPATHOLOGY Volume: 61 Issue: 3 Pages: 395-408 Published: SEP 2012 Times Cited: 19 25. CYTOKERATIN EXPRESSION DURING REGENERATION OF THE INTRALOBULAR DUCT IN RAT SUBMANDIBULAR GLANDS AFTER YAG LASER IRRADIATION By: TAKAHASHI, S; WAKITA, M ARCHIVES OF HISTOLOGY AND CYTOLOGY Volume: 57 Issue: 2 Pages: 167-173 Published: MAY 1994 Times Cited: 9 26. Epidermal growth factor in the treatment of diabetic foot ulcers: an update. (View record in MEDLINE) By: Tiaka, Elisavet K; Papanas, Nikolaos; Manolakis, Anastassios C; et al. Perspectives in vascular surgery and endovascular therapy Volume: 24 Issue: 1 Pages: 37-44 Published: 2012-Mar (Epub 2012 Apr 11) Times Cited: 42 27. 'Substance' By: Williams, J PARIS REVIEW Issue: 148 Pages: 14-27 Published: FAL 1998