In-vivo study of Chrysin against Scopolamine induced dementia in rats

Abstract

Alzheimer's disease is a dementia type that lead to memory, behavior and thinking problems. It starts by developing symptoms slowly then get worsen. It is world wide spread disease about 1 of 9 persons over the age of 65 are suffering from Alzheimer's disease (AD). The aim of our study is to prove the therapeutic effect of chrysin in treating scopolamine induced dementia. Scopolamine is a muscarinic receptor blocker which antagonizes effect of acetylcholine in cortex and hippocampus. Scopolamine also increases acetylcholine esterase, tau protein and b-amyloid protein levels which cause impaired cognition and cholinergic dysfunction. Chrysin is a hydroxylated flavone derivative found in many planets such as honey and propolis. Chrysin has a protective effect in brain through dampening the free radical generation which cause destruction of neurons, inhibits the release of pro-inflammatory cytokines such as Tumor necrosis factor-a (TNF-a) and interleukin- 1b (IL-1b) in lipopolysaccharide (LPS)-stimulated microglia. The experimental design of the study consist of thirty male albino rats will be divided into 5 groups (6 rats for each group).Group one was the normal group, Group two was injected by scopolamine in 1 mg/kg dose for seven days in order to induction of Alzheimer’s disease, Group three received donepezil in 2.5 mg/kg once daily for seven days as a standard group, Group 4: was pre-treated with Chrysin and Group 5 received Chrysin with donepezil at the same time. Parameters will be measured are ACHE, NFr2, HO-1, amyloid B and GSK3 expression. Finally, after administration of Chrysin, donepezil and combination between the two drugs for the drugs for 14 days, the results show a significally decreased in acetyl choline esterase levels and amyloid beta levels for the treated group (Chrysin, donepezil, and combination) while show a significally increased in scopolamine group. On the other hand, the heme oxgynase-1 and Nrf2 was significally increased in acetyl choline esterase levels and amyloid beta levels for the treated group (Chrysin, donepezil, and combination) while show a significally decreased in scopolamine group. Also, Western blot results shows that the expression of GSK3 in the treated group was significantly higher than the scopolamine group. However, Chrysin was shown to increased Gsk3 expression. o Abstract