The Biological activity of Conocarpus erectus extracts and their application as cytotoxic agents
No Thumbnail Available
Date
2018
Authors
Talaat Helmy Othman Ali, Aseel
Journal Title
Journal ISSN
Volume Title
Type
Other
Publisher
October University for Modern Sciences and Arts
Series Info
Doi
Scientific Journal Rankings
Abstract
Essential oils are found to have multiple active components which can show in vitro
cytotoxic action against various cancerous cell lines. This study reports the in vitro cytotoxic
effects of the essential oil from Conocarpus erectus (Combretaceae) growing wild in Egypt.
Water-distilled essential oil of C. erectus was examined for its cytotoxic effects using a modified
brine shrimp and MTT assays. Fresh leaves aerial part of C. erectus was subjected to hydro
distillation using a Clevenger-type apparatusvolatile to obtain its volatile oil. Cytotoxicity of the
essential oil was measured against HepG2 cancer cells and brine shrimps larva. The essential oil
50% cytotoxic concentrations were found to be 33μg/ml and 8.7μg/ml against brine shrimp and
human liver carcinoma HepG2 cell line, respectively; thus the volatile oil displayed good cytotoxic
action against the human tumor cell line. Moreover, C. erectus methanol extract was very effective;
it exhibited cytotoxic activity against brine shrimp larva within IC50 value of 15μg/ml. The
investigation from GC Mass, led to the identification of 12 constituents, representing 97.53% of
the total oil, of which the major chemical constituents were identified by gas chromatography
mass spectrometry as being rich in 3-(2,2 dimethylpropylid ene)bicyclo[3.3.1]nonane-2,4-dione
(3) (67.12%), (decanoic acid derevatives (11) (7.77%), 22-tritetracontanone (12) (6.03%), 1-
octanol, 2-butyl- (2) (5.51%) and oleic acid (6) (4.33%). This is the first report on anticancer
potential and separation of essential oils from C. erectus. The findings of this study necessitate
the need for further consideration of this essential oil in anti-neoplastic chemotherapy.
Description
Keywords
جامعة أكتوبرللعلوم الحديثة و الأداب, October University for Modern Sciences and Arts, University for Modern Sciences and Arts, MSA University, Conocarpus erectus, cytotoxicity, MTT assay, HepG2 cells, volatile oil, phytochemistry
Citation
Copyright © 2019 MSA University. All Rights Reserved.