ZnO nano-solvent foe efficacy enhancenemnt of some antibiotics in poultry
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Date
2020
Authors
Emadaldin Salah Kassem, Emadaldin
Journal Title
Journal ISSN
Volume Title
Type
Other
Publisher
October University for Modern Sciences and Arts
Series Info
Faculty Of Biotechnology Graduation Project 2020;
Doi
Scientific Journal Rankings
Abstract
Nowadays, bacterial resistance to antibiotics has become a global issue. Nano materials, such as metal oxide nanoparticles, have appeared to be promising contenders during the last few years. The aim of this study was to investigate zinc oxide (ZnO) nanoparticles in vitro antibacterial properties against avian pathogenic E. coli alone and in combination with different antibiotics. In this study, ZnO nanoparticle was synthesized using direct precipitation method. Physical characteristics of ZnO NPs were confirmed using X-Ray diffraction (XRD) and Transmission Electron Microscopy (TEM). Antibacterial resistance pattern of ten antibiotics: (amoxicillin(Aml), ciprofloxacin (Cip), enrofloxacin (Ex), gentamicin (Cn), doxycycline (Do), levofloxacin (LEV), Trimethoprim/Sulfamethoxazole (SXT), tetracyclin (TE), Spiramycin(SR) and Streptomycin(S)), in addition to different concentration of ZnO NPs, was determined by disc diffusion assay on 10 avian pathogenic E. coli (APEC). The results showed that 50% (5\10) of the strains are resistant to all antibiotic, while the rest were found to be sensitive to one or two antibiotics. The best concentration of ZnO NPs was 1gm\ ml which give wider zone than other concentrations (0.5, 0.25, 0.125, 0.0625 and 0.03125 gm \ml). The combination between antibiotic and ZnO NPs showed a synergistic effect with spiramycin, gentamycin and streptomycin while an antagonistic effect was observed between ZnO NPs and Ciprofloxacin and Enrofloxacin. There was no effect with other antibiotic. However, our findings, the in vivo evaluation should be evaluated to confirm the results
Description
Faculty Of Biotechnology Graduation Project 2019 - 2020
Keywords
MSA, October University for Modern Sciences and Arts, University for Modern Sciences and Arts, جامعة أكتوبر للعلوم الحديثة والآداب, Biotechnology, antibiotics
Citation
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