Influence of exogenous application of proline and glycinebetaine on antioxidative system of salt- stressed basil plants

Abstract

Basil plant has many uses ,it is used for cooking ,enters in the cosmetics industry and used to make medicine .Salinity stress affect crop productivity worldwide ,it affects plant growth by reducing .Salinity stress causes physiological and metabolic stress .It affects the chemical composition of the plant’s leaves by reducing carbohydrates ,proteins and minerals .Proline and glycine betaine increase in various plant as a natural defense mechanism against different abiotic stresses .The exogenous application of proline and glycine betaine shows positive impact in many plant ,it increases the growth rate of the plant and the chemicals in the leaf such as carbohydrates ,proteins and minerals .This projects aims to test the effect of the exogenous application of proline and glycine betaine in the improvement of basil plant growth under salinity stress .The height ,the weight and branches number will be compared .The chemical composition of the leaves carbohydrate ,proteins will be tested .Different concentration of proline and glycine betaine will be tested ( 50 mM ,100 mM ,200 mM ) .Under the concentration of 100 NaCL :the best height was 53.85 cm under 200 mM of glycine betaine compared to 40.61 of the control .The highest number of branches was observed is 6.21 under the 200 mM of glycine betaine in comparison with 4.31 of the control .The highest weight was 149.76 g under the 200 mM of glycine betaine in comparison to 123.76 g of the control. The highest conc. of carbohydrates was 3.21 mM under 200 mM of proline in comparison to 3.21 mM of the control. The highest conc. was 3.62 mM under 200 mM of proline in comparison to 3.22 mM of the control. The highest conc. of nitrogen was 0.588 mM under 50 mM of glycine 6 betaine in comparison to 0.51 mM of the control. The highest conc. of calcium was 155.9 mM under the 50 mM of glycine betaine in comparison to 136.43 mM of the control. The highest number of iron was 3.59 mM under 100 mM of proline in comparison to 2.94 mM of the control