Detection of the adulteration and commercial fraud with Pig meat and fat in the processed meat and chocolate stuff using Real Time PCR and chemical analysis

Abstract

Human health is an important matter so we should protect our health from contaminants that found in the environment. Eating untreated Pork and lard leading to disease that affect human health such as heart diseases, diabetes type 2, nausea, diarrhea, vomiting, abdominal pain, muscle pain, fever and swelling of the face. Pig related substances that are untreated or not cooked adequately could enter many food markets by fraud through several ways it can cause health problems upon consumption. It can lead to the infection by Trichinellosis which is a parasitic infection caused by Trichinella nematodes. The aim of this study is identification of fraud in different food samples. In this study, fraud contamination level was determined in 32 raw food samples (meat and chocolate) collected from farms, markets, butchers, and retail markets in different localities of the Greater Giza using Real-Time PCR (RT-PCR). Assay of Real-Time PCR was developed, for the highly specific detection of pork DNA. For the assay, the mtDNA of 12S RNA-tRNA Val gene was amplified at 63 ̊ C was amplified at 63 ̊ C using SYBR Green I for 45 min with a Real-Time PCR System that measured the fluorescent signal at one-minute intervals. Moreover, the DNA sequences of the isolated samples were characterized. The Fraud in food samples were detected in ten samples which represent about 31.25% of the tested samples.