Screening of the antimicrobial effect of Lactobacillus isolates from dairy products on Staphylococcus aureus isolates.

Abstract

Probiotics are live beneficial microorganism which can maintain human health when applied . Because of its inhibitory and potential antimicrobial effect against various kinds of bacteria and some human pathogenic strains, Lactobacillus is found to be the best type of probiotics to be studied . It can be found in milk and cheese ( C. Dunne, L. O'Mahony, 2001 ). Lactobacillus is the most commonly used probiotic in foods including yogurt and its use is extensive in preserving human health because it may help to treat menstrual diseases and skin eczema. It also produces antimicrobial peptides (bacteriocins) active against several pathogens (U. Schillinger, 1999) .Lactobacillus is found to has an antimicrobial effect against Staphylococcus aureus. Staphylococcus aureus is gram-positive bacteria which are cocci-like and are typically arranged in grape-like clusters. Its a considerable bacterial disease causing a wide range of clinical manifestations. These organisms may develop at 18 C to 40 C temperatures and can grow aerobically or anaerobically. Therapy is still challenging because of multi-drug resistant strains such as MRSA (Staphylococcus aureus methicillin-resistant) is a multi-drug resistant line. S. Aureus is also found in normal human flora, located on the skin and mucous membranes. Consequently, our aim of this research is to investigate antimicrobial effect of Lactobacillus isolates from dairy products Staphylococcus aureus isolates. Achieving research aim can be carried out through the following steps: Isolation of Lactobacillus from different milk products, Identification of the isolates by Gram staining and biochemical reactions, Investigating the antimicrobial effect of Lactobacillus isolates on the isolates of Staphylococcus aureus (Walker SJ, Archer P,1990) .